Single-laser shot fluorescence lifetime imaging on the nanosecond timescale using a Dual Image and Modeling Evaluation algorithm
(2012) In Optics Express 20(3). p.3043-3056- Abstract
- A novel technique, designated dual imaging and modeling evaluation (DIME), for evaluating single-laser shot fluorescence lifetimes is presented. The technique is experimentally verified in a generic gas mixing experiment to provide a clear demonstration of the rapidness and sensitivity of the detector scheme. Single-laser shot fluorescence lifetimes of roughly 800 ps with a standard deviation of similar to 120 ps were determined. These results were compared to streak camera measurements. Furthermore, a general fluorescence lifetime determination algorithm is proposed. The evaluation algorithm has an analytic, linear relationship between the fluorescence lifetime and detector signal ratio. In combination with the DIME detector scheme, it is... (More)
- A novel technique, designated dual imaging and modeling evaluation (DIME), for evaluating single-laser shot fluorescence lifetimes is presented. The technique is experimentally verified in a generic gas mixing experiment to provide a clear demonstration of the rapidness and sensitivity of the detector scheme. Single-laser shot fluorescence lifetimes of roughly 800 ps with a standard deviation of similar to 120 ps were determined. These results were compared to streak camera measurements. Furthermore, a general fluorescence lifetime determination algorithm is proposed. The evaluation algorithm has an analytic, linear relationship between the fluorescence lifetime and detector signal ratio. In combination with the DIME detector scheme, it is a faster, more accurate and more sensitive approach for rapid fluorescence lifetime imaging than previously proposed techniques. Monte Carlo simulations were conducted to analyze the sensitivity of the detector scheme as well as to compare the proposed evaluation algorithm to previously presented rapid lifetime determination algorithms. (C) 2012 Optical Society of America (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2517441
- author
- Ehn, Andreas LU ; Johansson, Olof LU ; Arvidsson, Andreas LU ; Aldén, Marcus LU and Bood, Joakim LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Optics Express
- volume
- 20
- issue
- 3
- pages
- 3043 - 3056
- publisher
- Optical Society of America
- external identifiers
-
- wos:000300499500110
- scopus:84856489312
- ISSN
- 1094-4087
- DOI
- 10.1364/OE.20.003043
- language
- English
- LU publication?
- yes
- id
- 82c95543-a380-4ce6-8826-00896e6551d2 (old id 2517441)
- date added to LUP
- 2016-04-01 14:59:47
- date last changed
- 2022-04-22 06:14:59
@article{82c95543-a380-4ce6-8826-00896e6551d2, abstract = {{A novel technique, designated dual imaging and modeling evaluation (DIME), for evaluating single-laser shot fluorescence lifetimes is presented. The technique is experimentally verified in a generic gas mixing experiment to provide a clear demonstration of the rapidness and sensitivity of the detector scheme. Single-laser shot fluorescence lifetimes of roughly 800 ps with a standard deviation of similar to 120 ps were determined. These results were compared to streak camera measurements. Furthermore, a general fluorescence lifetime determination algorithm is proposed. The evaluation algorithm has an analytic, linear relationship between the fluorescence lifetime and detector signal ratio. In combination with the DIME detector scheme, it is a faster, more accurate and more sensitive approach for rapid fluorescence lifetime imaging than previously proposed techniques. Monte Carlo simulations were conducted to analyze the sensitivity of the detector scheme as well as to compare the proposed evaluation algorithm to previously presented rapid lifetime determination algorithms. (C) 2012 Optical Society of America}}, author = {{Ehn, Andreas and Johansson, Olof and Arvidsson, Andreas and Aldén, Marcus and Bood, Joakim}}, issn = {{1094-4087}}, language = {{eng}}, number = {{3}}, pages = {{3043--3056}}, publisher = {{Optical Society of America}}, series = {{Optics Express}}, title = {{Single-laser shot fluorescence lifetime imaging on the nanosecond timescale using a Dual Image and Modeling Evaluation algorithm}}, url = {{https://lup.lub.lu.se/search/files/4291188/2969042.pdf}}, doi = {{10.1364/OE.20.003043}}, volume = {{20}}, year = {{2012}}, }