Effect of reserpine on the generation of the chromogranin A-derived neuropeptide WE-14 in rat oxyntic mucosa
(2005) In Regulatory Peptides 124(1-3). p.1-6- Abstract
- WE-14, a post-translational product of the neuroendocrine protein chromogranin A (CgA), is generated in distinct subpopulations of endocrine cells. The objective of this study was to investigate the generation of WE-14 in the endocrine cell types of the oxyntic mucosa of the stomach, after treatment with reserpine, an irreversible inhibitor of vesicular monoamine uptake 2 (VMAT2). Reserpine (10 mg/kg) was administered subcutaneously and tissue analysed 1, 3, 5 and 18 h following treatment. The oxymic mucosa was analysed immunohistochemically employing a site-specific WE-14 antiserum, a region-specific CgA antiserum and an antiserum against histidine decarboxylase (HDC), a marker of the histamine-producing ECL cells in the oxyntic mucosa.... (More)
- WE-14, a post-translational product of the neuroendocrine protein chromogranin A (CgA), is generated in distinct subpopulations of endocrine cells. The objective of this study was to investigate the generation of WE-14 in the endocrine cell types of the oxyntic mucosa of the stomach, after treatment with reserpine, an irreversible inhibitor of vesicular monoamine uptake 2 (VMAT2). Reserpine (10 mg/kg) was administered subcutaneously and tissue analysed 1, 3, 5 and 18 h following treatment. The oxymic mucosa was analysed immunohistochemically employing a site-specific WE-14 antiserum, a region-specific CgA antiserum and an antiserum against histidine decarboxylase (HDC), a marker of the histamine-producing ECL cells in the oxyntic mucosa. The number of oxyntic endocrine cells exhibiting WE-14 immunostaining increased more than 100-fold 18 h after reserpine administration relative to vehicle treated controls. Double immunostaining with HDC revealed that most, but not all, of the WE-14 positive cells were ECL cells. These results suggest that reserpine has the ability to influence the post-translational processing of CgA to generate WE-14 in rat stomach ECL cells, presumably as a consequence of reduced VMAT2-driven accumulation of histamine. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/259106
- author
- Quinn, JG ; Curry, WJ and Norlén, Per LU
- organization
- publishing date
- 2005
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Regulatory Peptides
- volume
- 124
- issue
- 1-3
- pages
- 1 - 6
- publisher
- Elsevier
- external identifiers
-
- wos:000225638300001
- pmid:15544835
- scopus:8444247960
- pmid:15544835
- ISSN
- 1873-1686
- DOI
- 10.1016/j.regpep.2004.06.028
- language
- English
- LU publication?
- yes
- id
- cbb001ee-1da6-4c3d-b946-ddaec3fa0148 (old id 259106)
- date added to LUP
- 2016-04-01 11:41:02
- date last changed
- 2022-01-26 08:39:23
@article{cbb001ee-1da6-4c3d-b946-ddaec3fa0148,
abstract = {{WE-14, a post-translational product of the neuroendocrine protein chromogranin A (CgA), is generated in distinct subpopulations of endocrine cells. The objective of this study was to investigate the generation of WE-14 in the endocrine cell types of the oxyntic mucosa of the stomach, after treatment with reserpine, an irreversible inhibitor of vesicular monoamine uptake 2 (VMAT2). Reserpine (10 mg/kg) was administered subcutaneously and tissue analysed 1, 3, 5 and 18 h following treatment. The oxymic mucosa was analysed immunohistochemically employing a site-specific WE-14 antiserum, a region-specific CgA antiserum and an antiserum against histidine decarboxylase (HDC), a marker of the histamine-producing ECL cells in the oxyntic mucosa. The number of oxyntic endocrine cells exhibiting WE-14 immunostaining increased more than 100-fold 18 h after reserpine administration relative to vehicle treated controls. Double immunostaining with HDC revealed that most, but not all, of the WE-14 positive cells were ECL cells. These results suggest that reserpine has the ability to influence the post-translational processing of CgA to generate WE-14 in rat stomach ECL cells, presumably as a consequence of reduced VMAT2-driven accumulation of histamine.}},
author = {{Quinn, JG and Curry, WJ and Norlén, Per}},
issn = {{1873-1686}},
language = {{eng}},
number = {{1-3}},
pages = {{1--6}},
publisher = {{Elsevier}},
series = {{Regulatory Peptides}},
title = {{Effect of reserpine on the generation of the chromogranin A-derived neuropeptide WE-14 in rat oxyntic mucosa}},
url = {{http://dx.doi.org/10.1016/j.regpep.2004.06.028}},
doi = {{10.1016/j.regpep.2004.06.028}},
volume = {{124}},
year = {{2005}},
}