The intracellular helical bundle of human glucose transporter GLUT4 is important for complex formation with ASPL
(2023) In FEBS Open Bio 13(11). p.2094-2107- Abstract
Glucose transporters (GLUTs) are responsible for transporting hexose molecules across cellular membranes. In adipocytes, insulin stimulates glucose uptake by redistributing GLUT4 to the plasma membrane. In unstimulated adipose-like mouse cell lines, GLUT4 is known to be retained intracellularly by binding to TUG protein, while upon insulin stimulation, GLUT4 dissociates from TUG. Here, we report that the TUG homolog in human, ASPL, exerts similar properties, i.e., forms a complex with GLUT4. We describe the structural details of complex formation by combining biochemical assays with cross-linking mass spectrometry and computational modeling. Combined, the data suggest that the intracellular domain of GLUT4 binds to the helical lariat of... (More)
Glucose transporters (GLUTs) are responsible for transporting hexose molecules across cellular membranes. In adipocytes, insulin stimulates glucose uptake by redistributing GLUT4 to the plasma membrane. In unstimulated adipose-like mouse cell lines, GLUT4 is known to be retained intracellularly by binding to TUG protein, while upon insulin stimulation, GLUT4 dissociates from TUG. Here, we report that the TUG homolog in human, ASPL, exerts similar properties, i.e., forms a complex with GLUT4. We describe the structural details of complex formation by combining biochemical assays with cross-linking mass spectrometry and computational modeling. Combined, the data suggest that the intracellular domain of GLUT4 binds to the helical lariat of ASPL and contributes to the regulation of GLUT4 trafficking by cooperative binding.
(Less)
- author
- Huang, Peng
LU
; Åbacka, Hannah
LU
; Varela, Daniel LU
; Venskutonytė, Raminta LU ; Happonen, Lotta LU ; Bogan, Jonathan S. ; Gourdon, Pontus LU ; Amiry-Moghaddam, Mahmood R. ; André, Ingmar and Lindkvist-Petersson, Karin LU
- organization
- publishing date
- 2023
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- adipocyte, ASPL, glucose transporters, GLUT4, trafficking, TUG
- in
- FEBS Open Bio
- volume
- 13
- issue
- 11
- pages
- 2094 - 2107
- publisher
- Wiley-Blackwell
- external identifiers
-
- pmid:37731227
- scopus:85173974503
- ISSN
- 2211-5463
- DOI
- 10.1002/2211-5463.13709
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2023 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies.
- id
- 262c2831-91d8-4d97-9f92-55d6e268bd85
- date added to LUP
- 2023-10-31 05:52:32
- date last changed
- 2024-08-09 13:06:37
@article{262c2831-91d8-4d97-9f92-55d6e268bd85, abstract = {{<p>Glucose transporters (GLUTs) are responsible for transporting hexose molecules across cellular membranes. In adipocytes, insulin stimulates glucose uptake by redistributing GLUT4 to the plasma membrane. In unstimulated adipose-like mouse cell lines, GLUT4 is known to be retained intracellularly by binding to TUG protein, while upon insulin stimulation, GLUT4 dissociates from TUG. Here, we report that the TUG homolog in human, ASPL, exerts similar properties, i.e., forms a complex with GLUT4. We describe the structural details of complex formation by combining biochemical assays with cross-linking mass spectrometry and computational modeling. Combined, the data suggest that the intracellular domain of GLUT4 binds to the helical lariat of ASPL and contributes to the regulation of GLUT4 trafficking by cooperative binding.</p>}}, author = {{Huang, Peng and Åbacka, Hannah and Varela, Daniel and Venskutonytė, Raminta and Happonen, Lotta and Bogan, Jonathan S. and Gourdon, Pontus and Amiry-Moghaddam, Mahmood R. and André, Ingmar and Lindkvist-Petersson, Karin}}, issn = {{2211-5463}}, keywords = {{adipocyte; ASPL; glucose transporters; GLUT4; trafficking; TUG}}, language = {{eng}}, number = {{11}}, pages = {{2094--2107}}, publisher = {{Wiley-Blackwell}}, series = {{FEBS Open Bio}}, title = {{The intracellular helical bundle of human glucose transporter GLUT4 is important for complex formation with ASPL}}, url = {{http://dx.doi.org/10.1002/2211-5463.13709}}, doi = {{10.1002/2211-5463.13709}}, volume = {{13}}, year = {{2023}}, }