Generation of human iPS cell line CTL07-II from human fibroblasts, under defined and xeno-free conditions
(2016) In Stem Cell Research 17(3). p.474-478- Abstract
CTL07-II is a healthy feeder-free and characterized human induced pluripotent stem (iPS) cell line. Cultured under xeno-free and defined conditions. The line is generated from healthy human fibroblasts with non-integrating Sendai virus vectors encoding the four Yamanaka factors, OCT4, SOX2, KLF4 and cMYC. The generated iPS cells are free from reprogramming vectors and their purity, karyotypic stability and pluripotent capacity is confirmed.
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https://lup.lub.lu.se/record/2727a117-88b8-40f1-a6d0-51d54cefd528
- author
- Kele, Malin ; Day, Kelly ; Rönnholm, Harriet ; Schuster, Jens ; Dahl, Niklas and Falk, Anna LU
- publishing date
- 2016-11
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Cell Differentiation, Cell Line, Cellular Reprogramming, Culture Media/chemistry, Embryoid Bodies/cytology, Fibroblasts/cytology, Genetic Vectors/genetics, Humans, Induced Pluripotent Stem Cells/cytology, Karyotype, Male, Microscopy, Fluorescence, Sendai virus/genetics, Transcription Factors/genetics
- in
- Stem Cell Research
- volume
- 17
- issue
- 3
- pages
- 5 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:27789397
- scopus:85024376878
- ISSN
- 1876-7753
- DOI
- 10.1016/j.scr.2016.09.028
- language
- English
- LU publication?
- no
- id
- 2727a117-88b8-40f1-a6d0-51d54cefd528
- date added to LUP
- 2021-08-10 13:01:04
- date last changed
- 2024-09-22 22:39:17
@article{2727a117-88b8-40f1-a6d0-51d54cefd528, abstract = {{<p>CTL07-II is a healthy feeder-free and characterized human induced pluripotent stem (iPS) cell line. Cultured under xeno-free and defined conditions. The line is generated from healthy human fibroblasts with non-integrating Sendai virus vectors encoding the four Yamanaka factors, OCT4, SOX2, KLF4 and cMYC. The generated iPS cells are free from reprogramming vectors and their purity, karyotypic stability and pluripotent capacity is confirmed.</p>}}, author = {{Kele, Malin and Day, Kelly and Rönnholm, Harriet and Schuster, Jens and Dahl, Niklas and Falk, Anna}}, issn = {{1876-7753}}, keywords = {{Cell Differentiation; Cell Line; Cellular Reprogramming; Culture Media/chemistry; Embryoid Bodies/cytology; Fibroblasts/cytology; Genetic Vectors/genetics; Humans; Induced Pluripotent Stem Cells/cytology; Karyotype; Male; Microscopy, Fluorescence; Sendai virus/genetics; Transcription Factors/genetics}}, language = {{eng}}, number = {{3}}, pages = {{474--478}}, publisher = {{Elsevier}}, series = {{Stem Cell Research}}, title = {{Generation of human iPS cell line CTL07-II from human fibroblasts, under defined and xeno-free conditions}}, url = {{https://lup.lub.lu.se/search/files/101075400/Generation_of_human_iPS_cell_line.pdf}}, doi = {{10.1016/j.scr.2016.09.028}}, volume = {{17}}, year = {{2016}}, }