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H4K20me0 recognition by BRCA1–BARD1 directs homologous recombination to sister chromatids

Nakamura, Kyosuke ; Saredi, Giulia LU ; Becker, Jordan R. ; Foster, Benjamin M. ; Nguyen, Nhuong V. ; Beyer, Tracey E. ; Cesa, Laura C. ; Faull, Peter A. ; Lukauskas, Saulius and Frimurer, Thomas , et al. (2019) In Nature Cell Biology 21(3). p.311-318
Abstract

Genotoxic DNA double-strand breaks (DSBs) can be repaired by error-free homologous recombination (HR) or mutagenic non-homologous end-joining 1 . HR supresses tumorigenesis 1 , but is restricted to the S and G2 phases of the cell cycle when a sister chromatid is present 2 . Breast cancer type 1 susceptibility protein (BRCA1) promotes HR by antagonizing the anti-resection factor TP53-binding protein 1(53BP1) (refs. 2–5 ), but it remains unknown... (More)

Genotoxic DNA double-strand breaks (DSBs) can be repaired by error-free homologous recombination (HR) or mutagenic non-homologous end-joining 1 . HR supresses tumorigenesis 1 , but is restricted to the S and G2 phases of the cell cycle when a sister chromatid is present 2 . Breast cancer type 1 susceptibility protein (BRCA1) promotes HR by antagonizing the anti-resection factor TP53-binding protein 1(53BP1) (refs. 2–5 ), but it remains unknown how BRCA1 function is limited to the S and G2 phases. We show that BRCA1 recruitment requires recognition of histone H4 unmethylated at lysine 20 (H4K20me0), linking DSB repair pathway choice directly to sister chromatid availability. We identify the ankyrin repeat domain of BRCA1-associated RING domain protein 1 (BARD1)—the obligate BRCA1 binding partner 3 —as a reader of H4K20me0 present on new histones in post-replicative chromatin 6 . BARD1 ankyrin repeat domain mutations disabling H4K20me0 recognition abrogate accumulation of BRCA1 at DSBs, causing aberrant build-up of 53BP1, and allowing anti-resection activity to prevail in S and G2. Consequently, BARD1 recognition of H4K20me0 is required for HR and resistance to poly (ADP-ribose) polymerase inhibitors. Collectively, this reveals that BRCA1–BARD1 monitors the replicative state of the genome to oppose 53BP1 function, routing only DSBs within sister chromatids to HR.

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publishing date
type
Contribution to journal
publication status
published
in
Nature Cell Biology
volume
21
issue
3
pages
311 - 318
publisher
Nature Publishing Group
external identifiers
  • scopus:85062074147
  • pmid:30804502
ISSN
1465-7392
DOI
10.1038/s41556-019-0282-9
language
English
LU publication?
no
additional info
Publisher Copyright: © 2019, The Author(s), under exclusive licence to Springer Nature Limited.
id
2780c8de-a578-4e86-b10f-60ac39ac833b
date added to LUP
2025-11-03 11:03:57
date last changed
2025-11-03 11:37:59
@article{2780c8de-a578-4e86-b10f-60ac39ac833b,
  abstract     = {{<p>                             Genotoxic DNA double-strand breaks (DSBs) can be repaired by error-free homologous recombination (HR) or mutagenic non-homologous end-joining                             <sup>1</sup>                             . HR supresses tumorigenesis                             <sup>1</sup>                             , but is restricted to the S and G2 phases of the cell cycle when a sister chromatid is present                             <sup>2</sup>                             . Breast cancer type 1 susceptibility protein (BRCA1) promotes HR by antagonizing the anti-resection factor TP53-binding protein 1(53BP1) (refs.                              <sup>2–5</sup>                             ), but it remains unknown how BRCA1 function is limited to the S and G2 phases. We show that BRCA1 recruitment requires recognition of histone H4 unmethylated at lysine 20 (H4K20me0), linking DSB repair pathway choice directly to sister chromatid availability. We identify the ankyrin repeat domain of BRCA1-associated RING domain protein 1 (BARD1)—the obligate BRCA1 binding partner                             <sup>3</sup>                             —as a reader of H4K20me0 present on new histones in post-replicative chromatin                             <sup>6</sup>                             . BARD1 ankyrin repeat domain mutations disabling H4K20me0 recognition abrogate accumulation of BRCA1 at DSBs, causing aberrant build-up of 53BP1, and allowing anti-resection activity to prevail in S and G2. Consequently, BARD1 recognition of H4K20me0 is required for HR and resistance to poly (ADP-ribose) polymerase inhibitors. Collectively, this reveals that BRCA1–BARD1 monitors the replicative state of the genome to oppose 53BP1 function, routing only DSBs within sister chromatids to HR.</p>}},
  author       = {{Nakamura, Kyosuke and Saredi, Giulia and Becker, Jordan R. and Foster, Benjamin M. and Nguyen, Nhuong V. and Beyer, Tracey E. and Cesa, Laura C. and Faull, Peter A. and Lukauskas, Saulius and Frimurer, Thomas and Chapman, J. Ross and Bartke, Till and Groth, Anja}},
  issn         = {{1465-7392}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{3}},
  pages        = {{311--318}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Cell Biology}},
  title        = {{H4K20me0 recognition by BRCA1–BARD1 directs homologous recombination to sister chromatids}},
  url          = {{http://dx.doi.org/10.1038/s41556-019-0282-9}},
  doi          = {{10.1038/s41556-019-0282-9}},
  volume       = {{21}},
  year         = {{2019}},
}