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Immunization with the recombinant Burkholderia pseudomallei outer membrane protein Omp85 induces protective immunity in mice

Su, Shanice Yc LU ; Wan, Kiew-Lian and Nathan, Sheila (2010) In Vaccine 28(31). p.5005-5011
Abstract
Burkholderia pseudomallei is resistant to a wide range of antibiotics, leading to relapse and recrudescence of melioidosis after cessation of antibiotic therapy. More effective immunotherapies are needed for better management of melioidosis. We evaluated the prophylactic potential of the immunogenic outer membrane protein Omp85 as a vaccine against murine melioidosis. Immunization of BALB/c mice with recombinant Omp85 (rOmp85) triggered a Th2-type immune response. Up to 70% of the immunized animals were protected against infectious challenge of B. pseudomallei with reduced bacterial load in extrapulmonary organs. Mouse anti-rOmp85 promoted complement-mediated killing and opsonophagocytosis of B. pseudomallei by human polymorphonuclear... (More)
Burkholderia pseudomallei is resistant to a wide range of antibiotics, leading to relapse and recrudescence of melioidosis after cessation of antibiotic therapy. More effective immunotherapies are needed for better management of melioidosis. We evaluated the prophylactic potential of the immunogenic outer membrane protein Omp85 as a vaccine against murine melioidosis. Immunization of BALB/c mice with recombinant Omp85 (rOmp85) triggered a Th2-type immune response. Up to 70% of the immunized animals were protected against infectious challenge of B. pseudomallei with reduced bacterial load in extrapulmonary organs. Mouse anti-rOmp85 promoted complement-mediated killing and opsonophagocytosis of B. pseudomallei by human polymorphonuclear cells. In conclusion, we demonstrated that B. pseudomallei Omp85 is potentially able to induce protective immunity against melioidosis. (Less)
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author
; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Vaccine
volume
28
issue
31
pages
5005 - 5011
publisher
Elsevier
external identifiers
  • scopus:77954760485
ISSN
1873-2518
DOI
10.1016/j.vaccine.2010.05.022
language
English
LU publication?
yes
id
27fa018a-591c-4133-8796-cb211339595f
date added to LUP
2019-06-19 14:24:54
date last changed
2025-04-04 15:24:07
@article{27fa018a-591c-4133-8796-cb211339595f,
  abstract     = {{Burkholderia pseudomallei is resistant to a wide range of antibiotics, leading to relapse and recrudescence of melioidosis after cessation of antibiotic therapy. More effective immunotherapies are needed for better management of melioidosis. We evaluated the prophylactic potential of the immunogenic outer membrane protein Omp85 as a vaccine against murine melioidosis. Immunization of BALB/c mice with recombinant Omp85 (rOmp85) triggered a Th2-type immune response. Up to 70% of the immunized animals were protected against infectious challenge of B. pseudomallei with reduced bacterial load in extrapulmonary organs. Mouse anti-rOmp85 promoted complement-mediated killing and opsonophagocytosis of B. pseudomallei by human polymorphonuclear cells. In conclusion, we demonstrated that B. pseudomallei Omp85 is potentially able to induce protective immunity against melioidosis.}},
  author       = {{Su, Shanice Yc and Wan, Kiew-Lian and Nathan, Sheila}},
  issn         = {{1873-2518}},
  language     = {{eng}},
  month        = {{07}},
  number       = {{31}},
  pages        = {{5005--5011}},
  publisher    = {{Elsevier}},
  series       = {{Vaccine}},
  title        = {{Immunization with the recombinant Burkholderia pseudomallei outer membrane protein Omp85 induces protective immunity in mice}},
  url          = {{http://dx.doi.org/10.1016/j.vaccine.2010.05.022}},
  doi          = {{10.1016/j.vaccine.2010.05.022}},
  volume       = {{28}},
  year         = {{2010}},
}