Next-generation phylogeography of the banded newts (Ommatotriton) : A phylogenetic hypothesis for three ancient species with geographically restricted interspecific gene flow and deep intraspecific genetic structure
(2022) In Molecular Phylogenetics and Evolution 167. p.107361-107361- Abstract
Technological developments now make it possible to employ many markers for many individuals in a phylogeographic setting, even for taxa with large and complex genomes such as salamanders. The banded newt (genus Ommatotriton) from the Near East has been proposed to contain three species (O. nesterovi, O. ophryticus and O. vittatus) with unclear phylogenetic relationships, apparently limited interspecific gene flow and deep intraspecific geographic mtDNA structure. We use parallel tagged amplicon sequencing to obtain 177 nuclear DNA markers for 35 banded newts sampled throughout the range. We determine population structure (with Bayesian clustering and principal component analysis), interspecific gene flow (by determining the distribution... (More)
Technological developments now make it possible to employ many markers for many individuals in a phylogeographic setting, even for taxa with large and complex genomes such as salamanders. The banded newt (genus Ommatotriton) from the Near East has been proposed to contain three species (O. nesterovi, O. ophryticus and O. vittatus) with unclear phylogenetic relationships, apparently limited interspecific gene flow and deep intraspecific geographic mtDNA structure. We use parallel tagged amplicon sequencing to obtain 177 nuclear DNA markers for 35 banded newts sampled throughout the range. We determine population structure (with Bayesian clustering and principal component analysis), interspecific gene flow (by determining the distribution of species-diagnostic alleles) and phylogenetic relationships (by maximum likelihood inference of concatenated sequence data and based on a summary-coalescent approach). We confirm that the three proposed species are genetically distinct. A sister relationship between O. nesterovi and O. ophryticus is suggested. We find evidence for introgression between O. nesterovi and O. ophryticus, but this is geographically limited. Intraspecific structuring is extensive, with the only recognized banded newt subspecies, O. vittatus cilicensis, representing the most distinct lineage below the species level. While mtDNA mostly mirrors the pattern observed in nuclear DNA, all banded newt species show mito-nuclear discordance as well.
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- author
- van Riemsdijk, Isolde LU ; Arntzen, Jan W ; Babik, Wiesław ; Bogaerts, Sergé ; Franzen, Michael ; Kalaentzis, Konstantinos ; Litvinchuk, Spartak N ; Olgun, Kurtuluş ; Wijnands, Jan Willem P M and Wielstra, Ben
- publishing date
- 2022-02-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Animals, Bayes Theorem, DNA, Mitochondrial/genetics, Gene Flow, Genetic Structures, Phylogeny, Phylogeography, Salamandridae/classification, Sequence Analysis, DNA
- in
- Molecular Phylogenetics and Evolution
- volume
- 167
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:34775056
- scopus:85119328902
- ISSN
- 1095-9513
- DOI
- 10.1016/j.ympev.2021.107361
- language
- English
- LU publication?
- no
- additional info
- Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
- id
- 283a29a7-aed4-4978-857c-a0024d6f96fd
- date added to LUP
- 2023-05-01 08:57:29
- date last changed
- 2024-12-14 22:14:42
@article{283a29a7-aed4-4978-857c-a0024d6f96fd,
abstract = {{<p>Technological developments now make it possible to employ many markers for many individuals in a phylogeographic setting, even for taxa with large and complex genomes such as salamanders. The banded newt (genus Ommatotriton) from the Near East has been proposed to contain three species (O. nesterovi, O. ophryticus and O. vittatus) with unclear phylogenetic relationships, apparently limited interspecific gene flow and deep intraspecific geographic mtDNA structure. We use parallel tagged amplicon sequencing to obtain 177 nuclear DNA markers for 35 banded newts sampled throughout the range. We determine population structure (with Bayesian clustering and principal component analysis), interspecific gene flow (by determining the distribution of species-diagnostic alleles) and phylogenetic relationships (by maximum likelihood inference of concatenated sequence data and based on a summary-coalescent approach). We confirm that the three proposed species are genetically distinct. A sister relationship between O. nesterovi and O. ophryticus is suggested. We find evidence for introgression between O. nesterovi and O. ophryticus, but this is geographically limited. Intraspecific structuring is extensive, with the only recognized banded newt subspecies, O. vittatus cilicensis, representing the most distinct lineage below the species level. While mtDNA mostly mirrors the pattern observed in nuclear DNA, all banded newt species show mito-nuclear discordance as well.</p>}},
author = {{van Riemsdijk, Isolde and Arntzen, Jan W and Babik, Wiesław and Bogaerts, Sergé and Franzen, Michael and Kalaentzis, Konstantinos and Litvinchuk, Spartak N and Olgun, Kurtuluş and Wijnands, Jan Willem P M and Wielstra, Ben}},
issn = {{1095-9513}},
keywords = {{Animals; Bayes Theorem; DNA, Mitochondrial/genetics; Gene Flow; Genetic Structures; Phylogeny; Phylogeography; Salamandridae/classification; Sequence Analysis, DNA}},
language = {{eng}},
month = {{02}},
pages = {{107361--107361}},
publisher = {{Elsevier}},
series = {{Molecular Phylogenetics and Evolution}},
title = {{Next-generation phylogeography of the banded newts (Ommatotriton) : A phylogenetic hypothesis for three ancient species with geographically restricted interspecific gene flow and deep intraspecific genetic structure}},
url = {{http://dx.doi.org/10.1016/j.ympev.2021.107361}},
doi = {{10.1016/j.ympev.2021.107361}},
volume = {{167}},
year = {{2022}},
}