Human TRPA1 is an inherently mechanosensitive bilayer-gated ion channel
(2020) In Cell Calcium 91.- Abstract
The role of mammalian Transient Receptor Potential Ankyrin 1 (TRPA1) as a mechanosensor is controversial. Here, we report that purified human TRPA1 (hTRPA1) with and without its N-terminal ankyrin repeat domain responded with pressure-dependent single-channel current activity when reconstituted into artificial lipid bilayers. The hTRPA1 activity was abolished by the thiol reducing agent TCEP. Thus, depending on its redox state, hTRPA1 is an inherent mechanosensitive ion channel gated by force-from-lipids.
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/29fbec85-1a8b-40b3-8cdc-24ad160823df
- author
- Moparthi, Lavanya
LU
and Zygmunt, Peter M.
LU
- organization
- publishing date
- 2020
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Mechanosensation, Mechanosensitive channel, Redox sensitivity, TRP channel, TRPA1
- in
- Cell Calcium
- volume
- 91
- article number
- 102255
- publisher
- Elsevier
- external identifiers
-
- pmid:32717533
- scopus:85088385173
- ISSN
- 0143-4160
- DOI
- 10.1016/j.ceca.2020.102255
- language
- English
- LU publication?
- yes
- id
- 29fbec85-1a8b-40b3-8cdc-24ad160823df
- date added to LUP
- 2020-08-04 09:10:12
- date last changed
- 2024-06-26 19:50:28
@article{29fbec85-1a8b-40b3-8cdc-24ad160823df, abstract = {{<p>The role of mammalian Transient Receptor Potential Ankyrin 1 (TRPA1) as a mechanosensor is controversial. Here, we report that purified human TRPA1 (hTRPA1) with and without its N-terminal ankyrin repeat domain responded with pressure-dependent single-channel current activity when reconstituted into artificial lipid bilayers. The hTRPA1 activity was abolished by the thiol reducing agent TCEP. Thus, depending on its redox state, hTRPA1 is an inherent mechanosensitive ion channel gated by force-from-lipids.</p>}}, author = {{Moparthi, Lavanya and Zygmunt, Peter M.}}, issn = {{0143-4160}}, keywords = {{Mechanosensation; Mechanosensitive channel; Redox sensitivity; TRP channel; TRPA1}}, language = {{eng}}, publisher = {{Elsevier}}, series = {{Cell Calcium}}, title = {{Human TRPA1 is an inherently mechanosensitive bilayer-gated ion channel}}, url = {{http://dx.doi.org/10.1016/j.ceca.2020.102255}}, doi = {{10.1016/j.ceca.2020.102255}}, volume = {{91}}, year = {{2020}}, }