Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Human TRPA1 is an inherently mechanosensitive bilayer-gated ion channel

Moparthi, Lavanya LU and Zygmunt, Peter M. LU orcid (2020) In Cell Calcium 91.
Abstract

The role of mammalian Transient Receptor Potential Ankyrin 1 (TRPA1) as a mechanosensor is controversial. Here, we report that purified human TRPA1 (hTRPA1) with and without its N-terminal ankyrin repeat domain responded with pressure-dependent single-channel current activity when reconstituted into artificial lipid bilayers. The hTRPA1 activity was abolished by the thiol reducing agent TCEP. Thus, depending on its redox state, hTRPA1 is an inherent mechanosensitive ion channel gated by force-from-lipids.

Please use this url to cite or link to this publication:
author
and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Mechanosensation, Mechanosensitive channel, Redox sensitivity, TRP channel, TRPA1
in
Cell Calcium
volume
91
article number
102255
publisher
Elsevier
external identifiers
  • pmid:32717533
  • scopus:85088385173
ISSN
0143-4160
DOI
10.1016/j.ceca.2020.102255
language
English
LU publication?
yes
id
29fbec85-1a8b-40b3-8cdc-24ad160823df
date added to LUP
2020-08-04 09:10:12
date last changed
2024-06-26 19:50:28
@article{29fbec85-1a8b-40b3-8cdc-24ad160823df,
  abstract     = {{<p>The role of mammalian Transient Receptor Potential Ankyrin 1 (TRPA1) as a mechanosensor is controversial. Here, we report that purified human TRPA1 (hTRPA1) with and without its N-terminal ankyrin repeat domain responded with pressure-dependent single-channel current activity when reconstituted into artificial lipid bilayers. The hTRPA1 activity was abolished by the thiol reducing agent TCEP. Thus, depending on its redox state, hTRPA1 is an inherent mechanosensitive ion channel gated by force-from-lipids.</p>}},
  author       = {{Moparthi, Lavanya and Zygmunt, Peter M.}},
  issn         = {{0143-4160}},
  keywords     = {{Mechanosensation; Mechanosensitive channel; Redox sensitivity; TRP channel; TRPA1}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Cell Calcium}},
  title        = {{Human TRPA1 is an inherently mechanosensitive bilayer-gated ion channel}},
  url          = {{http://dx.doi.org/10.1016/j.ceca.2020.102255}},
  doi          = {{10.1016/j.ceca.2020.102255}},
  volume       = {{91}},
  year         = {{2020}},
}