Strengths and weaknesses of the aniline-blue method used to test mushroom (1→3)-β-D-glucans obtained by microwave-assisted extractions
(2019) In Carbohydrate Polymers 217. p.135-143- Abstract
The parameters to extract polysaccharide-enriched fractions (PEF) from mushrooms using MAE (microwave-assisted extraction) were adjusted following a full factorial 3
2
experimental design. The highest yield and total carbohydrate values, using Lentinula edodes as model mushroom, were obtained at 180 °C and 30 min. Several mushroom species were submitted to MAE and their PEF yields ranged between 12.1–44.2%. (1→3)-β-Glucans determination using a conventional fluorimetric method changed depending on the standard utilized. NMR analyses of PEF... (More)
(Less)
The parameters to extract polysaccharide-enriched fractions (PEF) from mushrooms using MAE (microwave-assisted extraction) were adjusted following a full factorial 3
2
experimental design. The highest yield and total carbohydrate values, using Lentinula edodes as model mushroom, were obtained at 180 °C and 30 min. Several mushroom species were submitted to MAE and their PEF yields ranged between 12.1–44.2%. (1→3)-β-Glucans determination using a conventional fluorimetric method changed depending on the standard utilized. NMR analyses of PEF indicated that the presence of other polysaccharides in the extracts or their specific folding, might impair the proper determination of (1→3) linkages by the fluorophore. Mushrooms from Cantharellales order contained (1→3)-β-glucans but they were not detected with the fluorimetric method. Therefore, although the method (after adjustments) was sensitive enough to detect their presence in many mushroom extracts, it cannot be used for all species and it is also not recommended for quantitative determinations.
- author
- Gil-Ramírez, Alicia LU ; Smiderle, Fhernanda Ribeiro ; Morales, Diego ; Iacomini, Marcello and Soler-Rivas, Cristina
- organization
- publishing date
- 2019
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- (1→3)-β-D-glucans, Fluorimetric assay, MAE, Microwave-assisted extraction, Mushrooms, Polysaccharides
- in
- Carbohydrate Polymers
- volume
- 217
- pages
- 9 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:31079669
- scopus:85064509742
- ISSN
- 0144-8617
- DOI
- 10.1016/j.carbpol.2019.04.051
- language
- English
- LU publication?
- yes
- id
- 2a1c39e7-08c2-43db-afaa-ac48cb09f3ff
- date added to LUP
- 2019-05-02 14:36:09
- date last changed
- 2024-09-17 18:44:24
@article{2a1c39e7-08c2-43db-afaa-ac48cb09f3ff, abstract = {{<p><br> The parameters to extract polysaccharide-enriched fractions (PEF) from mushrooms using MAE (microwave-assisted extraction) were adjusted following a full factorial 3 <br> <sup>2</sup><br> experimental design. The highest yield and total carbohydrate values, using Lentinula edodes as model mushroom, were obtained at 180 °C and 30 min. Several mushroom species were submitted to MAE and their PEF yields ranged between 12.1–44.2%. (1→3)-β-Glucans determination using a conventional fluorimetric method changed depending on the standard utilized. NMR analyses of PEF indicated that the presence of other polysaccharides in the extracts or their specific folding, might impair the proper determination of (1→3) linkages by the fluorophore. Mushrooms from Cantharellales order contained (1→3)-β-glucans but they were not detected with the fluorimetric method. Therefore, although the method (after adjustments) was sensitive enough to detect their presence in many mushroom extracts, it cannot be used for all species and it is also not recommended for quantitative determinations. <br> </p>}}, author = {{Gil-Ramírez, Alicia and Smiderle, Fhernanda Ribeiro and Morales, Diego and Iacomini, Marcello and Soler-Rivas, Cristina}}, issn = {{0144-8617}}, keywords = {{(1→3)-β-D-glucans; Fluorimetric assay; MAE; Microwave-assisted extraction; Mushrooms; Polysaccharides}}, language = {{eng}}, pages = {{135--143}}, publisher = {{Elsevier}}, series = {{Carbohydrate Polymers}}, title = {{Strengths and weaknesses of the aniline-blue method used to test mushroom (1→3)-β-D-glucans obtained by microwave-assisted extractions}}, url = {{http://dx.doi.org/10.1016/j.carbpol.2019.04.051}}, doi = {{10.1016/j.carbpol.2019.04.051}}, volume = {{217}}, year = {{2019}}, }