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Antigen restriction and IgG subclasses among anti-GBM autoantibodies

Segelmark, Marten LU ; Butkowski, R. and Wieslander, J. LU (1990) In Nephrology Dialysis Transplantation 5(12). p.991-996
Abstract

Thirty-seven sera positive for anti-GBM antibodies (antibodies against the ‘Goodpasture antigen’ = NCI-domain of collagen IV) in routine analysis were studied for antigen restriction and IgG subclasses. Four different polypeptides, the four α-chains (α1, α2, α3 and α4) of human collagen IV NCI, were used as coating in ELISA assays. Autoantibodies were detected with monoclonal antibodies towards human IgG subclasses. All patients had antibodies to the α3 chain, and most patients reacted much more to the α3 peptide than to any of the other three peptides. This shows that the NCI domain of the α3 chain of collagen IV is the major target for anti-GBM antibodies. A minor group of patients, 6 of 37,... (More)

Thirty-seven sera positive for anti-GBM antibodies (antibodies against the ‘Goodpasture antigen’ = NCI-domain of collagen IV) in routine analysis were studied for antigen restriction and IgG subclasses. Four different polypeptides, the four α-chains (α1, α2, α3 and α4) of human collagen IV NCI, were used as coating in ELISA assays. Autoantibodies were detected with monoclonal antibodies towards human IgG subclasses. All patients had antibodies to the α3 chain, and most patients reacted much more to the α3 peptide than to any of the other three peptides. This shows that the NCI domain of the α3 chain of collagen IV is the major target for anti-GBM antibodies. A minor group of patients, 6 of 37, showed no antigen restriction and had only moderate titres. It remains to be studied, however, whether they have antibodies to another epitope and a different clinical picture. All four subclasses of IgG antibodies were present, but IgGl antibodies dominated. A group, consisting of females mainly, had relatively high titres of IgG4 antibodies to the NCI domain of the α3 chain of collagen IV.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Anti-GBM, Collagen IV, Glomerulonephritis, Goodpasture's syndrome, IgG subclasses
in
Nephrology Dialysis Transplantation
volume
5
issue
12
pages
991 - 996
publisher
Oxford University Press
external identifiers
  • scopus:0025651367
  • pmid:2128958
ISSN
0931-0509
DOI
10.1093/ndt/5.12.991
language
English
LU publication?
yes
id
2baabc83-7a95-40d2-954c-8bcfee3cdea1
date added to LUP
2020-05-19 16:50:12
date last changed
2024-02-16 16:27:59
@article{2baabc83-7a95-40d2-954c-8bcfee3cdea1,
  abstract     = {{<p>Thirty-seven sera positive for anti-GBM antibodies (antibodies against the ‘Goodpasture antigen’ = NCI-domain of collagen IV) in routine analysis were studied for antigen restriction and IgG subclasses. Four different polypeptides, the four α-chains (α<sub>1</sub>, α<sub>2</sub>, α<sub>3</sub> and α<sub>4</sub>) of human collagen IV NCI, were used as coating in ELISA assays. Autoantibodies were detected with monoclonal antibodies towards human IgG subclasses. All patients had antibodies to the α3 chain, and most patients reacted much more to the α3 peptide than to any of the other three peptides. This shows that the NCI domain of the α3 chain of collagen IV is the major target for anti-GBM antibodies. A minor group of patients, 6 of 37, showed no antigen restriction and had only moderate titres. It remains to be studied, however, whether they have antibodies to another epitope and a different clinical picture. All four subclasses of IgG antibodies were present, but IgGl antibodies dominated. A group, consisting of females mainly, had relatively high titres of IgG4 antibodies to the NCI domain of the α3 chain of collagen IV.</p>}},
  author       = {{Segelmark, Marten and Butkowski, R. and Wieslander, J.}},
  issn         = {{0931-0509}},
  keywords     = {{Anti-GBM; Collagen IV; Glomerulonephritis; Goodpasture's syndrome; IgG subclasses}},
  language     = {{eng}},
  month        = {{01}},
  number       = {{12}},
  pages        = {{991--996}},
  publisher    = {{Oxford University Press}},
  series       = {{Nephrology Dialysis Transplantation}},
  title        = {{Antigen restriction and IgG subclasses among anti-GBM autoantibodies}},
  url          = {{http://dx.doi.org/10.1093/ndt/5.12.991}},
  doi          = {{10.1093/ndt/5.12.991}},
  volume       = {{5}},
  year         = {{1990}},
}