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Transcription factor GATA6 promotes migration of human coronary artery smooth muscle cells in vitro

Alajbegovic, Azra LU ; Daoud, Fatima LU orcid ; Ali, Neserin LU orcid ; Kawka, Katarzyna LU ; Holmberg, Johan LU and Albinsson, Sebastian LU (2022) In Frontiers in Physiology 13.
Abstract

Vascular smooth muscle cell plasticity plays a pivotal role in the pathophysiology of vascular diseases. Despite compelling evidence demonstrating the importance of transcription factor GATA6 in vascular smooth muscle, the functional role of GATA6 remains poorly understood. The aim of this study was to elucidate the role of GATA6 on cell migration and to gain insight into GATA6-sensitive genes in smooth muscle. We found that overexpression of GATA6 promotes migration of human coronary artery smooth muscle cells in vitro, and that silencing of GATA6 in smooth muscle cells resulted in reduced cellular motility. Furthermore, a complete microarray screen of GATA6-sensitive gene transcription resulted in 739 upregulated and 248 downregulated... (More)

Vascular smooth muscle cell plasticity plays a pivotal role in the pathophysiology of vascular diseases. Despite compelling evidence demonstrating the importance of transcription factor GATA6 in vascular smooth muscle, the functional role of GATA6 remains poorly understood. The aim of this study was to elucidate the role of GATA6 on cell migration and to gain insight into GATA6-sensitive genes in smooth muscle. We found that overexpression of GATA6 promotes migration of human coronary artery smooth muscle cells in vitro, and that silencing of GATA6 in smooth muscle cells resulted in reduced cellular motility. Furthermore, a complete microarray screen of GATA6-sensitive gene transcription resulted in 739 upregulated and 248 downregulated genes. Pathways enrichment analysis showed involvement of transforming growth factor beta (TGF-β) signaling which was validated by measuring mRNA expression level of several members. Furthermore, master regulators prediction based on microarray data revealed several members of (mitogen activated protein kinase) MAPK pathway as a master regulators, reflecting involvement of MAPK pathway also. Our findings provide further insights into the functional role of GATA6 in vascular smooth muscle and suggest that targeting GATA6 may constitute as a new approach to inhibit vascular smooth muscle migration.

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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cell migration, GATA6, microarray, phenotypic modulation, vascular smooth muscle
in
Frontiers in Physiology
volume
13
article number
1054819
publisher
Frontiers Media S. A.
external identifiers
  • scopus:85143886656
ISSN
1664-042X
DOI
10.3389/fphys.2022.1054819
language
English
LU publication?
yes
additional info
Publisher Copyright: Copyright © 2022 Alajbegovic, Daoud, Ali, Kawka, Holmberg and Albinsson.
id
2ec8629d-17a5-4f9f-ab14-622f651a84dc
date added to LUP
2026-07-02 15:41:45
date last changed
2026-07-02 15:42:23
@article{2ec8629d-17a5-4f9f-ab14-622f651a84dc,
  abstract     = {{<p>Vascular smooth muscle cell plasticity plays a pivotal role in the pathophysiology of vascular diseases. Despite compelling evidence demonstrating the importance of transcription factor GATA6 in vascular smooth muscle, the functional role of GATA6 remains poorly understood. The aim of this study was to elucidate the role of GATA6 on cell migration and to gain insight into GATA6-sensitive genes in smooth muscle. We found that overexpression of GATA6 promotes migration of human coronary artery smooth muscle cells in vitro, and that silencing of GATA6 in smooth muscle cells resulted in reduced cellular motility. Furthermore, a complete microarray screen of GATA6-sensitive gene transcription resulted in 739 upregulated and 248 downregulated genes. Pathways enrichment analysis showed involvement of transforming growth factor beta (TGF-β) signaling which was validated by measuring mRNA expression level of several members. Furthermore, master regulators prediction based on microarray data revealed several members of (mitogen activated protein kinase) MAPK pathway as a master regulators, reflecting involvement of MAPK pathway also. Our findings provide further insights into the functional role of GATA6 in vascular smooth muscle and suggest that targeting GATA6 may constitute as a new approach to inhibit vascular smooth muscle migration.</p>}},
  author       = {{Alajbegovic, Azra and Daoud, Fatima and Ali, Neserin and Kawka, Katarzyna and Holmberg, Johan and Albinsson, Sebastian}},
  issn         = {{1664-042X}},
  keywords     = {{cell migration; GATA6; microarray; phenotypic modulation; vascular smooth muscle}},
  language     = {{eng}},
  month        = {{11}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Physiology}},
  title        = {{Transcription factor GATA6 promotes migration of human coronary artery smooth muscle cells in vitro}},
  url          = {{http://dx.doi.org/10.3389/fphys.2022.1054819}},
  doi          = {{10.3389/fphys.2022.1054819}},
  volume       = {{13}},
  year         = {{2022}},
}