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Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism

Jensen, Benjamin A H ; Holm, Jacob B ; Larsen, Ida S ; von Burg, Nicole ; Derer, Stefanie ; Sonne, Si B ; Pærregaard, Simone I ; Damgaard, Mads V ; Indrelid, Stine A and Rivollier, Aymeric LU , et al. (2021) In Nature Communications 12(1).
Abstract

Interactions between host and gut microbial communities are modulated by diets and play pivotal roles in immunological homeostasis and health. We show that exchanging the protein source in a high fat, high sugar, westernized diet from casein to whole-cell lysates of the non-commensal bacterium Methylococcus capsulatus Bath is sufficient to reverse western diet-induced changes in the gut microbiota to a state resembling that of lean, low fat diet-fed mice, both under mild thermal stress (T22 °C) and at thermoneutrality (T30 °C). Concomitant with microbiota changes, mice fed the Methylococcus-based western diet exhibit improved glucose regulation, reduced body and liver fat, and diminished hepatic immune infiltration. Intake of the... (More)

Interactions between host and gut microbial communities are modulated by diets and play pivotal roles in immunological homeostasis and health. We show that exchanging the protein source in a high fat, high sugar, westernized diet from casein to whole-cell lysates of the non-commensal bacterium Methylococcus capsulatus Bath is sufficient to reverse western diet-induced changes in the gut microbiota to a state resembling that of lean, low fat diet-fed mice, both under mild thermal stress (T22 °C) and at thermoneutrality (T30 °C). Concomitant with microbiota changes, mice fed the Methylococcus-based western diet exhibit improved glucose regulation, reduced body and liver fat, and diminished hepatic immune infiltration. Intake of the Methylococcu-based diet markedly boosts Parabacteroides abundances in a manner depending on adaptive immunity, and upregulates triple positive (Foxp3+RORγt+IL-17+) regulatory T cells in the small and large intestine. Collectively, these data point to the potential for leveraging the use of McB lysates to improve immunometabolic homeostasis.

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publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Diet, Forkhead Transcription Factors/immunology, Homeostasis/immunology, Interleukin-17/immunology, Intestine, Large/immunology, Intestine, Small/immunology, Male, Methylococcus capsulatus/chemistry, Mice, Inbred C57BL, Microbiota/immunology, Nuclear Receptor Subfamily 1, Group F, Member 3/immunology, Obesity/immunology, Proteins/immunology, T-Lymphocytes, Regulatory/immunology
in
Nature Communications
volume
12
issue
1
article number
1093
publisher
Nature Publishing Group
external identifiers
  • pmid:33597537
  • scopus:85101092638
ISSN
2041-1723
DOI
10.1038/s41467-021-21408-9
language
English
LU publication?
yes
additional info
These authors jointly supervised this work: Benjamin A. H. Jensen, Karsten Kristiansen, Tor E. Lea.
id
2f33c9a6-540f-4eba-a9c3-139a17b4de63
date added to LUP
2021-03-26 09:48:53
date last changed
2024-05-18 06:53:02
@article{2f33c9a6-540f-4eba-a9c3-139a17b4de63,
  abstract     = {{<p>Interactions between host and gut microbial communities are modulated by diets and play pivotal roles in immunological homeostasis and health. We show that exchanging the protein source in a high fat, high sugar, westernized diet from casein to whole-cell lysates of the non-commensal bacterium Methylococcus capsulatus Bath is sufficient to reverse western diet-induced changes in the gut microbiota to a state resembling that of lean, low fat diet-fed mice, both under mild thermal stress (T22 °C) and at thermoneutrality (T30 °C). Concomitant with microbiota changes, mice fed the Methylococcus-based western diet exhibit improved glucose regulation, reduced body and liver fat, and diminished hepatic immune infiltration. Intake of the Methylococcu-based diet markedly boosts Parabacteroides abundances in a manner depending on adaptive immunity, and upregulates triple positive (Foxp3+RORγt+IL-17+) regulatory T cells in the small and large intestine. Collectively, these data point to the potential for leveraging the use of McB lysates to improve immunometabolic homeostasis.</p>}},
  author       = {{Jensen, Benjamin A H and Holm, Jacob B and Larsen, Ida S and von Burg, Nicole and Derer, Stefanie and Sonne, Si B and Pærregaard, Simone I and Damgaard, Mads V and Indrelid, Stine A and Rivollier, Aymeric and Agrinier, Anne-Laure and Sulek, Karolina and Arnoldussen, Yke J and Fjære, Even and Marette, André and Angell, Inga L and Rudi, Knut and Treebak, Jonas T and Madsen, Lise and Åkesson, Caroline Piercey and Agace, William and Sina, Christian and Kleiveland, Charlotte R and Kristiansen, Karsten and Lea, Tor E}},
  issn         = {{2041-1723}},
  keywords     = {{Animals; Diet; Forkhead Transcription Factors/immunology; Homeostasis/immunology; Interleukin-17/immunology; Intestine, Large/immunology; Intestine, Small/immunology; Male; Methylococcus capsulatus/chemistry; Mice, Inbred C57BL; Microbiota/immunology; Nuclear Receptor Subfamily 1, Group F, Member 3/immunology; Obesity/immunology; Proteins/immunology; T-Lymphocytes, Regulatory/immunology}},
  language     = {{eng}},
  month        = {{02}},
  number       = {{1}},
  publisher    = {{Nature Publishing Group}},
  series       = {{Nature Communications}},
  title        = {{Lysates of Methylococcus capsulatus Bath induce a lean-like microbiota, intestinal FoxP3+RORγt+IL-17+ Tregs and improve metabolism}},
  url          = {{http://dx.doi.org/10.1038/s41467-021-21408-9}},
  doi          = {{10.1038/s41467-021-21408-9}},
  volume       = {{12}},
  year         = {{2021}},
}