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Microfluidics-Driven Manufacturing and Multiscale Analytical Characterization of Nanoparticle-Vesicle Hybrids

Cardellini, Jacopo ; Normak, Karl ; Gerlt, Michael LU orcid ; Makasewicz, Katarzyna LU ; Seiffert, Charlotte ; Capasso Palmiero, Umberto ; Ye, Suiying ; González Gómez, Manuel A. ; Piñero, Yolanda and Rivas, José , et al. (2024) In Advanced healthcare materials
Abstract

Coating synthetic nanoparticles (NPs) with lipid membranes is a promising approach to enhance the performance of nanomaterials in various biological applications, including therapeutic delivery to target organs. Current methods for achieving this coating often rely on bulk approaches which can result in low efficiency and poor reproducibility. Continuous processes coupled with quality control represent an attractive strategy to manufacture products with consistent attributes and high yields. Here, this concept is implemented by developing an acoustic microfluidic device together with an analytical platform to prepare nanoparticle-vesicle hybrids and quantitatively characterize the nanoparticle coverage using fluorescence-based... (More)

Coating synthetic nanoparticles (NPs) with lipid membranes is a promising approach to enhance the performance of nanomaterials in various biological applications, including therapeutic delivery to target organs. Current methods for achieving this coating often rely on bulk approaches which can result in low efficiency and poor reproducibility. Continuous processes coupled with quality control represent an attractive strategy to manufacture products with consistent attributes and high yields. Here, this concept is implemented by developing an acoustic microfluidic device together with an analytical platform to prepare nanoparticle-vesicle hybrids and quantitatively characterize the nanoparticle coverage using fluorescence-based techniques at different levels of resolution. With this approach polymethyl methacrylate (PMMA) nanoparticles are successfully coated with liposomes and extracellular vesicles (EVs), achieving a high encapsulation efficiency of 70%. Moreover, the approach enables the identification of design rules to control the efficiency of encapsulation by tuning various operational parameters and material properties, including buffer composition, nanoparticle/vesicle ratio, and vesicle rigidity.

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publishing date
type
Contribution to journal
publication status
epub
subject
keywords
acoustofluidics, lipid vesicles, microfluidics, nanoparticle-vesicle hybrids, nanoparticles
in
Advanced healthcare materials
article number
2403264
publisher
John Wiley & Sons Inc.
external identifiers
  • scopus:85212983143
  • pmid:39722148
ISSN
2192-2640
DOI
10.1002/adhm.202403264
language
English
LU publication?
no
additional info
Publisher Copyright: © 2024 The Author(s). Advanced Healthcare Materials published by Wiley-VCH GmbH.
id
2ff13b65-04a3-4a94-8020-0fc6ce54ff84
date added to LUP
2025-01-12 22:04:44
date last changed
2025-07-15 01:51:14
@article{2ff13b65-04a3-4a94-8020-0fc6ce54ff84,
  abstract     = {{<p>Coating synthetic nanoparticles (NPs) with lipid membranes is a promising approach to enhance the performance of nanomaterials in various biological applications, including therapeutic delivery to target organs. Current methods for achieving this coating often rely on bulk approaches which can result in low efficiency and poor reproducibility. Continuous processes coupled with quality control represent an attractive strategy to manufacture products with consistent attributes and high yields. Here, this concept is implemented by developing an acoustic microfluidic device together with an analytical platform to prepare nanoparticle-vesicle hybrids and quantitatively characterize the nanoparticle coverage using fluorescence-based techniques at different levels of resolution. With this approach polymethyl methacrylate (PMMA) nanoparticles are successfully coated with liposomes and extracellular vesicles (EVs), achieving a high encapsulation efficiency of 70%. Moreover, the approach enables the identification of design rules to control the efficiency of encapsulation by tuning various operational parameters and material properties, including buffer composition, nanoparticle/vesicle ratio, and vesicle rigidity.</p>}},
  author       = {{Cardellini, Jacopo and Normak, Karl and Gerlt, Michael and Makasewicz, Katarzyna and Seiffert, Charlotte and Capasso Palmiero, Umberto and Ye, Suiying and González Gómez, Manuel A. and Piñero, Yolanda and Rivas, José and Bongiovanni, Antonella and Bergese, Paolo and Arosio, Paolo}},
  issn         = {{2192-2640}},
  keywords     = {{acoustofluidics; lipid vesicles; microfluidics; nanoparticle-vesicle hybrids; nanoparticles}},
  language     = {{eng}},
  publisher    = {{John Wiley & Sons Inc.}},
  series       = {{Advanced healthcare materials}},
  title        = {{Microfluidics-Driven Manufacturing and Multiscale Analytical Characterization of Nanoparticle-Vesicle Hybrids}},
  url          = {{http://dx.doi.org/10.1002/adhm.202403264}},
  doi          = {{10.1002/adhm.202403264}},
  year         = {{2024}},
}