Quantitative PSA RT-PCR for preoperative staging of prostate cancer
Kurek, R ; Ylikoski, A ; Renneberg, H ; Konrad, L ; Aumuller, G ; Roddiger, SJ ; Zamboglou, N ; Tunn, UW and Lilja, Hans LU
(2003)
In The Prostate
56(4).
p.263-269
- Abstract
- BACKGROUND. The clinical value of detecting prostate specific antigen (PSA) mRNA in the peripheral blood mononuclear cell fraction of patients (pts) by standard RT-PCR assays with localized prostate cancer remains controversial. We used a quantitative RT-PCR assay to measure the PSA mRNA copy number in addition to the qualitative PSA RT-PCR and correlated the results with clinical parameters. METHODS. Total RNA was extracted from the peripheral blood mononuclear cell fraction of 115 prostate cancer pts prior to radical retropubic prostatectomy (RP) who received 3 months of neoadjuvant androgen deprivation. For quantitative RT-PCR, a PSA-like internal standard (IS) was added to each sample prior to reverse transcription and the PCR products... (More)
- BACKGROUND. The clinical value of detecting prostate specific antigen (PSA) mRNA in the peripheral blood mononuclear cell fraction of patients (pts) by standard RT-PCR assays with localized prostate cancer remains controversial. We used a quantitative RT-PCR assay to measure the PSA mRNA copy number in addition to the qualitative PSA RT-PCR and correlated the results with clinical parameters. METHODS. Total RNA was extracted from the peripheral blood mononuclear cell fraction of 115 prostate cancer pts prior to radical retropubic prostatectomy (RP) who received 3 months of neoadjuvant androgen deprivation. For quantitative RT-PCR, a PSA-like internal standard (IS) was added to each sample prior to reverse transcription and the PCR products for PSA and IS were selectively detected with fluorescent europium chelates; after hybridization. Corresponding qualitative PSA-RT-PCR was performed for all samples. RESULTS. The median PSA copy number was 126 (range: 0-37988). There were no significant correlations established between qualitative or quantitative RT-PCR results and given clinical parameters. Corresponding quantitative and qualitative RT-PCR results were significantly associated (P = 0.01). CONCLUSIONS. We were unable to show any additional value of quantitative as well as qualitative PSA RT-PCR for preoperative staging of prostate cancer so far. Nevertheless, the long-term follow up of the patients has to be awaited. Prostate 56:263-269,2003. (C) 2003 Wiley-Liss, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/303734
- author
- Kurek, R
; Ylikoski, A
; Renneberg, H
; Konrad, L
; Aumuller, G
; Roddiger, SJ
; Zamboglou, N
; Tunn, UW
and Lilja, Hans
LU
- organization
- publishing date
- 2003
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- prostate cancer, PSA, quantitative RT-PCR, molecular staging
- in
- The Prostate
- volume
- 56
- issue
- 4
- pages
- 263 - 269
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:12858354
- wos:000184741800004
- scopus:0043068093
- ISSN
- 0270-4137
- DOI
- 10.1002/pros.10257
- language
- English
- LU publication?
- yes
- id
- fc0904bd-c762-4bc5-97ab-f285670fe9d1 (old id 303734)
- date added to LUP
- 2016-04-01 11:36:26
- date last changed
- 2022-04-12 22:34:59
@article{fc0904bd-c762-4bc5-97ab-f285670fe9d1,
abstract = {{BACKGROUND. The clinical value of detecting prostate specific antigen (PSA) mRNA in the peripheral blood mononuclear cell fraction of patients (pts) by standard RT-PCR assays with localized prostate cancer remains controversial. We used a quantitative RT-PCR assay to measure the PSA mRNA copy number in addition to the qualitative PSA RT-PCR and correlated the results with clinical parameters. METHODS. Total RNA was extracted from the peripheral blood mononuclear cell fraction of 115 prostate cancer pts prior to radical retropubic prostatectomy (RP) who received 3 months of neoadjuvant androgen deprivation. For quantitative RT-PCR, a PSA-like internal standard (IS) was added to each sample prior to reverse transcription and the PCR products for PSA and IS were selectively detected with fluorescent europium chelates; after hybridization. Corresponding qualitative PSA-RT-PCR was performed for all samples. RESULTS. The median PSA copy number was 126 (range: 0-37988). There were no significant correlations established between qualitative or quantitative RT-PCR results and given clinical parameters. Corresponding quantitative and qualitative RT-PCR results were significantly associated (P = 0.01). CONCLUSIONS. We were unable to show any additional value of quantitative as well as qualitative PSA RT-PCR for preoperative staging of prostate cancer so far. Nevertheless, the long-term follow up of the patients has to be awaited. Prostate 56:263-269,2003. (C) 2003 Wiley-Liss, Inc.}},
author = {{Kurek, R and Ylikoski, A and Renneberg, H and Konrad, L and Aumuller, G and Roddiger, SJ and Zamboglou, N and Tunn, UW and Lilja, Hans}},
issn = {{0270-4137}},
keywords = {{prostate cancer; PSA; quantitative RT-PCR; molecular staging}},
language = {{eng}},
number = {{4}},
pages = {{263--269}},
publisher = {{John Wiley & Sons Inc.}},
series = {{The Prostate}},
title = {{Quantitative PSA RT-PCR for preoperative staging of prostate cancer}},
url = {{http://dx.doi.org/10.1002/pros.10257}},
doi = {{10.1002/pros.10257}},
volume = {{56}},
year = {{2003}},
}