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Phospholipase C is required for the control of stomatal aperture by ABA

Hunt, L ; Mills, LN ; Pical, Christophe LU ; Leckie, CP ; Aitken, FL ; Kopka, J ; Mueller-Roeber, B ; McAinsh, MR ; Hetherington, AM and Gray, JE (2003) In Plant Journal 34(1). p.47-55
Abstract
The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for... (More)
The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways. (Less)
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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
abscisic acid, guard cell, calcium, inositol 1, 4, 5-trisphosphate
in
Plant Journal
volume
34
issue
1
pages
47 - 55
publisher
Wiley-Blackwell
external identifiers
  • wos:000181862400005
  • pmid:12662308
  • scopus:0037800441
ISSN
1365-313X
DOI
10.1046/j.1365-313X.2003.01698.x
language
English
LU publication?
yes
id
e12cf620-c780-46df-adb2-25bd60e063fe (old id 315076)
date added to LUP
2016-04-01 16:53:51
date last changed
2022-02-28 00:27:08
@article{e12cf620-c780-46df-adb2-25bd60e063fe,
  abstract     = {{The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways.}},
  author       = {{Hunt, L and Mills, LN and Pical, Christophe and Leckie, CP and Aitken, FL and Kopka, J and Mueller-Roeber, B and McAinsh, MR and Hetherington, AM and Gray, JE}},
  issn         = {{1365-313X}},
  keywords     = {{abscisic acid; guard cell; calcium; inositol 1; 4; 5-trisphosphate}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{47--55}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{Plant Journal}},
  title        = {{Phospholipase C is required for the control of stomatal aperture by ABA}},
  url          = {{http://dx.doi.org/10.1046/j.1365-313X.2003.01698.x}},
  doi          = {{10.1046/j.1365-313X.2003.01698.x}},
  volume       = {{34}},
  year         = {{2003}},
}