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Antennal transcriptome analysis of the chemosensory gene families from Trichoptera and basal Lepidoptera

Yuvaraj, Jothi Kumar LU ; Andersson, Martin N LU ; Zhang, Dan-Dan LU and Löfstedt, Christer LU (2018) In Frontiers in Physiology 9.
Abstract
The chemosensory gene families of insects encode proteins that are crucial for host
location, mate finding, oviposition, and avoidance behaviors. The insect peripheral
chemosensory system comprises odorant receptors (ORs), gustatory receptors (GRs),
ionotropic receptors (IRs), odorant binding proteins (OBPs), chemosensory proteins
(CSPs), and sensory neuron membrane proteins (SNMPs). These protein families have
been identified from a large number of insect species, however, they still remain
unidentified from several taxa that could provide important clues to their evolution. These
taxa include older lepidopteran lineages and the sister order of Lepidoptera, Trichoptera
(caddisflies). Studies of these... (More)
The chemosensory gene families of insects encode proteins that are crucial for host
location, mate finding, oviposition, and avoidance behaviors. The insect peripheral
chemosensory system comprises odorant receptors (ORs), gustatory receptors (GRs),
ionotropic receptors (IRs), odorant binding proteins (OBPs), chemosensory proteins
(CSPs), and sensory neuron membrane proteins (SNMPs). These protein families have
been identified from a large number of insect species, however, they still remain
unidentified from several taxa that could provide important clues to their evolution. These
taxa include older lepidopteran lineages and the sister order of Lepidoptera, Trichoptera
(caddisflies). Studies of these insects should improve evolutionary analyses of insect
chemoreception, and in particular shed light on the origin of certain lepidopteran protein
subfamilies. These include the pheromone receptors (PRs) in the “PR clade”, the
pheromone binding proteins (PBPs), general odorant binding proteins (GOBPs), and
certain presumably Lepidoptera-specific IR subfamilies. Hence, we analyzed antennal
transcriptomes from Rhyacophila nubila (Trichoptera), Eriocrania semipurpurella, and
Lampronia capitella (representing two old lepidopteran lineages). We report 37 ORs,
17 IRs, 9 GRs, 30 OBPs, 7 CSPs, and 2 SNMPs in R. nubila; 37 ORs, 17 IRs, 3 GRs,
23 OBPs, 14 CSPs, and 2 SNMPs in E. semipurpurella; and 53 ORs, 20 IRs, 5 GRs,
29 OBPs, 17 CSPs, and 3 SNMPs in L. capitella. We identified IR members of the
“Lepidoptera-specific” subfamilies IR1 and IR87a also in R. nubila, demonstrating that
these IRs also occur in Trichoptera. Members of the GOBP subfamily were only found
in the two lepidopterans. ORs grouping within the PR clade, as well as PBPs, were only
found in L. capitella, a species that in contrast to R. nubila and E. semipurpurella uses
a so-called Type I pheromone similar to the pheromones of most species of the derived
Lepidoptera (Ditrysia). Thus, in addition to providing increased coverage for evolutionary
analyses of chemoreception in insects, our findings suggest that certain subfamilies of
chemosensory genes have evolved in parallel with the transition of sex pheromone types
in Lepidoptera. In addition, other chemoreceptor subfamilies show a broader taxonomic
occurrence than hitherto acknowledged. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
odorant receptor, membrane protein, sensory neuron, chemosensory protein
in
Frontiers in Physiology
volume
9
article number
1365
pages
16 pages
publisher
Frontiers Media S. A.
external identifiers
  • pmid:30319455
  • scopus:85071764702
ISSN
1664-042X
DOI
10.3389/fphys.2018.01365
project
Evolutionary mechanisms of pheromone divergence in Lepidoptera
language
English
LU publication?
yes
id
32088d38-a5a8-4c12-aa20-c66b89eca311
date added to LUP
2019-01-18 13:49:32
date last changed
2022-04-18 01:56:39
@article{32088d38-a5a8-4c12-aa20-c66b89eca311,
  abstract     = {{The chemosensory gene families of insects encode proteins that are crucial for host<br/>location, mate finding, oviposition, and avoidance behaviors. The insect peripheral<br/>chemosensory system comprises odorant receptors (ORs), gustatory receptors (GRs),<br/>ionotropic receptors (IRs), odorant binding proteins (OBPs), chemosensory proteins<br/>(CSPs), and sensory neuron membrane proteins (SNMPs). These protein families have<br/>been identified from a large number of insect species, however, they still remain<br/>unidentified from several taxa that could provide important clues to their evolution. These<br/>taxa include older lepidopteran lineages and the sister order of Lepidoptera, Trichoptera<br/>(caddisflies). Studies of these insects should improve evolutionary analyses of insect<br/>chemoreception, and in particular shed light on the origin of certain lepidopteran protein<br/>subfamilies. These include the pheromone receptors (PRs) in the “PR clade”, the<br/>pheromone binding proteins (PBPs), general odorant binding proteins (GOBPs), and<br/>certain presumably Lepidoptera-specific IR subfamilies. Hence, we analyzed antennal<br/>transcriptomes from Rhyacophila nubila (Trichoptera), Eriocrania semipurpurella, and<br/>Lampronia capitella (representing two old lepidopteran lineages). We report 37 ORs,<br/>17 IRs, 9 GRs, 30 OBPs, 7 CSPs, and 2 SNMPs in R. nubila; 37 ORs, 17 IRs, 3 GRs,<br/>23 OBPs, 14 CSPs, and 2 SNMPs in E. semipurpurella; and 53 ORs, 20 IRs, 5 GRs,<br/>29 OBPs, 17 CSPs, and 3 SNMPs in L. capitella. We identified IR members of the<br/>“Lepidoptera-specific” subfamilies IR1 and IR87a also in R. nubila, demonstrating that<br/>these IRs also occur in Trichoptera. Members of the GOBP subfamily were only found<br/>in the two lepidopterans. ORs grouping within the PR clade, as well as PBPs, were only<br/>found in L. capitella, a species that in contrast to R. nubila and E. semipurpurella uses<br/>a so-called Type I pheromone similar to the pheromones of most species of the derived<br/>Lepidoptera (Ditrysia). Thus, in addition to providing increased coverage for evolutionary<br/>analyses of chemoreception in insects, our findings suggest that certain subfamilies of<br/>chemosensory genes have evolved in parallel with the transition of sex pheromone types<br/>in Lepidoptera. In addition, other chemoreceptor subfamilies show a broader taxonomic<br/>occurrence than hitherto acknowledged.}},
  author       = {{Yuvaraj, Jothi Kumar and Andersson, Martin N and Zhang, Dan-Dan and Löfstedt, Christer}},
  issn         = {{1664-042X}},
  keywords     = {{odorant receptor; membrane protein; sensory neuron; chemosensory protein}},
  language     = {{eng}},
  month        = {{09}},
  publisher    = {{Frontiers Media S. A.}},
  series       = {{Frontiers in Physiology}},
  title        = {{Antennal transcriptome analysis of the chemosensory gene families from Trichoptera and basal Lepidoptera}},
  url          = {{http://dx.doi.org/10.3389/fphys.2018.01365}},
  doi          = {{10.3389/fphys.2018.01365}},
  volume       = {{9}},
  year         = {{2018}},
}