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Re-thinking translation quality control in bacteria : from trans-translation to collided-disome surveillance

Takada, Hiraku LU (2026) In Bioscience, biotechnology, and biochemistry 90(4). p.503-513
Abstract

Cells must recycle stalled ribosomes while preventing the accumulation of aberrant nascent chains. In bacteria, this is achieved by overlapping pathways with distinct substrates: ribosome-rescue systems act mainly on non-stop mRNAs, whereas ribosome-associated quality control (RQC) targets mid-ORF arrests. Work in Gram-positive bacteria defined an RQC mechanism that appends C-terminal degrons to stalled peptides, yet the full set of bacterial substrates and splitting factors remains unresolved, and enteric bacteria notably lack a canonical RQC elongation factor. This review traces the field from the discovery of tmRNA (also known as 10Sa RNA or SsrA RNA) through alternative rescue pathways to the current bacterial RQC framework. I... (More)

Cells must recycle stalled ribosomes while preventing the accumulation of aberrant nascent chains. In bacteria, this is achieved by overlapping pathways with distinct substrates: ribosome-rescue systems act mainly on non-stop mRNAs, whereas ribosome-associated quality control (RQC) targets mid-ORF arrests. Work in Gram-positive bacteria defined an RQC mechanism that appends C-terminal degrons to stalled peptides, yet the full set of bacterial substrates and splitting factors remains unresolved, and enteric bacteria notably lack a canonical RQC elongation factor. This review traces the field from the discovery of tmRNA (also known as 10Sa RNA or SsrA RNA) through alternative rescue pathways to the current bacterial RQC framework. I summarize mechanisms across three layers-processing of 50S-peptidyl-tRNA, collision sensing and splitting, and downstream proteolysis-and compare species-level strategies and conservation patterns. I highlight how rescue and quality control intersect during phage infection, and outline key mechanistic uncertainties and experiments needed to resolve them.

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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
ribosome, RQC, tmRNA, translation quality control
in
Bioscience, biotechnology, and biochemistry
volume
90
issue
4
pages
503 - 513
publisher
Oxford University Press
external identifiers
  • scopus:105034026670
  • pmid:41637055
ISSN
1347-6947
DOI
10.1093/bbb/zbag015
language
English
LU publication?
yes
additional info
Publisher Copyright: © The Author(s) 2026. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry. All rights reserved. For commercial re-use, please contact reprints@oup.com for reprints and translation rights for reprints. All other permissions can be obtained through our RightsLink service via the Permissions link on the article page on our site-for furthe
id
320debf4-95bf-4530-a586-361fd67a80dc
date added to LUP
2026-05-25 13:14:10
date last changed
2026-06-08 14:08:15
@article{320debf4-95bf-4530-a586-361fd67a80dc,
  abstract     = {{<p>Cells must recycle stalled ribosomes while preventing the accumulation of aberrant nascent chains. In bacteria, this is achieved by overlapping pathways with distinct substrates: ribosome-rescue systems act mainly on non-stop mRNAs, whereas ribosome-associated quality control (RQC) targets mid-ORF arrests. Work in Gram-positive bacteria defined an RQC mechanism that appends C-terminal degrons to stalled peptides, yet the full set of bacterial substrates and splitting factors remains unresolved, and enteric bacteria notably lack a canonical RQC elongation factor. This review traces the field from the discovery of tmRNA (also known as 10Sa RNA or SsrA RNA) through alternative rescue pathways to the current bacterial RQC framework. I summarize mechanisms across three layers-processing of 50S-peptidyl-tRNA, collision sensing and splitting, and downstream proteolysis-and compare species-level strategies and conservation patterns. I highlight how rescue and quality control intersect during phage infection, and outline key mechanistic uncertainties and experiments needed to resolve them.</p>}},
  author       = {{Takada, Hiraku}},
  issn         = {{1347-6947}},
  keywords     = {{ribosome; RQC; tmRNA; translation quality control}},
  language     = {{eng}},
  month        = {{03}},
  number       = {{4}},
  pages        = {{503--513}},
  publisher    = {{Oxford University Press}},
  series       = {{Bioscience, biotechnology, and biochemistry}},
  title        = {{Re-thinking translation quality control in bacteria : from trans-translation to collided-disome surveillance}},
  url          = {{http://dx.doi.org/10.1093/bbb/zbag015}},
  doi          = {{10.1093/bbb/zbag015}},
  volume       = {{90}},
  year         = {{2026}},
}