Characterization of a novel human endogenous retrovirus, HERV-H/F, expressed in human leukemia cell lines
(2002) In Virology 303(1). p.164-173- Abstract
- We have identified and characterized a human endogenous retrovirus (HERV) gag transcript in the human pre-B cell leukemia line Reh. The transcript was found to be a splice product of a structurally intact HERV element located on chromosome 6q13. Its primer binding site is complementary to phenylalanine (F) tRNA, common for the HERV-F family, but the overall genome sequence is closely related to the HERV-H family. The retroviral sequence was therefore designated HERV-H/F The HERV element shows a distinct mRNA expression pattern among hematopoietic cancer cell lines with expression in some leukemia-derived cell lines of B-lymphoid and myeloid origin. No expression was observed in normal human tissues, indicating a cancer-specific expression... (More)
- We have identified and characterized a human endogenous retrovirus (HERV) gag transcript in the human pre-B cell leukemia line Reh. The transcript was found to be a splice product of a structurally intact HERV element located on chromosome 6q13. Its primer binding site is complementary to phenylalanine (F) tRNA, common for the HERV-F family, but the overall genome sequence is closely related to the HERV-H family. The retroviral sequence was therefore designated HERV-H/F The HERV element shows a distinct mRNA expression pattern among hematopoietic cancer cell lines with expression in some leukemia-derived cell lines of B-lymphoid and myeloid origin. No expression was observed in normal human tissues, indicating a cancer-specific expression pattern. The 5' long terminal repeat (LTR) was tested for promoter activity in HERV-H/F expressing and nonexpressing cell lines. The cell specificity of the LTR-mediated reporter gene expression did not conclusively correlate with endogenous virus expression, indicating that the transcription regulation of this gene is not alone dependent on cell-specific activity of transcription factors. (C) 2002 Elsevier Science (USA). (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/321462
- author
- Patzke, S ; Lindeskog, Mats LU ; Munthe, E and Aasheim, HC
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- LTR, leukemia, HERV, endogenous retrovirus, expression
- in
- Virology
- volume
- 303
- issue
- 1
- pages
- 164 - 173
- publisher
- Elsevier
- external identifiers
-
- wos:000179828800016
- pmid:12482668
- scopus:0036924747
- ISSN
- 1096-0341
- DOI
- 10.1006/viro.2002.1615
- language
- English
- LU publication?
- yes
- id
- 1ce6fd9f-c3ba-4aed-a84b-7ffe482bbb08 (old id 321462)
- date added to LUP
- 2016-04-01 17:06:32
- date last changed
- 2022-02-13 02:48:31
@article{1ce6fd9f-c3ba-4aed-a84b-7ffe482bbb08,
abstract = {{We have identified and characterized a human endogenous retrovirus (HERV) gag transcript in the human pre-B cell leukemia line Reh. The transcript was found to be a splice product of a structurally intact HERV element located on chromosome 6q13. Its primer binding site is complementary to phenylalanine (F) tRNA, common for the HERV-F family, but the overall genome sequence is closely related to the HERV-H family. The retroviral sequence was therefore designated HERV-H/F The HERV element shows a distinct mRNA expression pattern among hematopoietic cancer cell lines with expression in some leukemia-derived cell lines of B-lymphoid and myeloid origin. No expression was observed in normal human tissues, indicating a cancer-specific expression pattern. The 5' long terminal repeat (LTR) was tested for promoter activity in HERV-H/F expressing and nonexpressing cell lines. The cell specificity of the LTR-mediated reporter gene expression did not conclusively correlate with endogenous virus expression, indicating that the transcription regulation of this gene is not alone dependent on cell-specific activity of transcription factors. (C) 2002 Elsevier Science (USA).}},
author = {{Patzke, S and Lindeskog, Mats and Munthe, E and Aasheim, HC}},
issn = {{1096-0341}},
keywords = {{LTR; leukemia; HERV; endogenous retrovirus; expression}},
language = {{eng}},
number = {{1}},
pages = {{164--173}},
publisher = {{Elsevier}},
series = {{Virology}},
title = {{Characterization of a novel human endogenous retrovirus, HERV-H/F, expressed in human leukemia cell lines}},
url = {{http://dx.doi.org/10.1006/viro.2002.1615}},
doi = {{10.1006/viro.2002.1615}},
volume = {{303}},
year = {{2002}},
}