Detection of specific Helicobacter pylori DNA and antigens in stool samples in dyspeptic patients and healthy subjects
(2002) In Microbiology and Immunology 46(10). p.657-665- Abstract
- In this study stool samples from dyspeptic patients and healthy subjects were used for detection of specific Helicobacter pylori antigens and DNA by immunoenzymatic test (PPHpSA) and semi-nested PCR (ureA-PCR), respectively. The H. pylori status was estimated by invasive endoscopy-based rapid urease test and histology or noninvasive urea breath test (UBT), and by serology (ELISA, Western blot). The coincidence of IT pylori-negative invasive tests or UBT and negative antigen or DNA stool tests was very high (mean 95%). The PPHpSA results were found positive for 56% and ureA-PCR for 26% of individuals with H. pylori infection confirmed by invasive tests or UBT. The detection of specific H. pylori antigens and especially DNA in feces is not... (More)
- In this study stool samples from dyspeptic patients and healthy subjects were used for detection of specific Helicobacter pylori antigens and DNA by immunoenzymatic test (PPHpSA) and semi-nested PCR (ureA-PCR), respectively. The H. pylori status was estimated by invasive endoscopy-based rapid urease test and histology or noninvasive urea breath test (UBT), and by serology (ELISA, Western blot). The coincidence of IT pylori-negative invasive tests or UBT and negative antigen or DNA stool tests was very high (mean 95%). The PPHpSA results were found positive for 56% and ureA-PCR for 26% of individuals with H. pylori infection confirmed by invasive tests or UBT. The detection of specific H. pylori antigens and especially DNA in feces is not sufficient as a one-step diagnosis of H. pylori infection. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/325986
- author
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Helicobacter pylori, diagnosis, stool antigens, PCR
- in
- Microbiology and Immunology
- volume
- 46
- issue
- 10
- pages
- 657 - 665
- publisher
- Center for Academic Publications Japan
- external identifiers
-
- pmid:12477244
- wos:000178508500002
- scopus:0036033379
- ISSN
- 1348-0421
- language
- English
- LU publication?
- yes
- id
- 1bb841cc-7d76-4e71-881e-0d02b0f953bd (old id 325986)
- alternative location
- http://www.jstage.jst.go.jp/article/mandi/46/10/657/_pdf
- date added to LUP
- 2016-04-01 12:15:41
- date last changed
- 2022-01-27 01:11:54
@article{1bb841cc-7d76-4e71-881e-0d02b0f953bd, abstract = {{In this study stool samples from dyspeptic patients and healthy subjects were used for detection of specific Helicobacter pylori antigens and DNA by immunoenzymatic test (PPHpSA) and semi-nested PCR (ureA-PCR), respectively. The H. pylori status was estimated by invasive endoscopy-based rapid urease test and histology or noninvasive urea breath test (UBT), and by serology (ELISA, Western blot). The coincidence of IT pylori-negative invasive tests or UBT and negative antigen or DNA stool tests was very high (mean 95%). The PPHpSA results were found positive for 56% and ureA-PCR for 26% of individuals with H. pylori infection confirmed by invasive tests or UBT. The detection of specific H. pylori antigens and especially DNA in feces is not sufficient as a one-step diagnosis of H. pylori infection.}}, author = {{Wisniewska, M and Nilsson, Hans-Olof and Bak-Romaniszyn, L and Rechcinski, T and Bielanski, W and Planeta-Malecka, I and Plonka, M and Konturek, S and Wadström, Torkel and Rudnicka, W and Chmiela, M}}, issn = {{1348-0421}}, keywords = {{Helicobacter pylori; diagnosis; stool antigens; PCR}}, language = {{eng}}, number = {{10}}, pages = {{657--665}}, publisher = {{Center for Academic Publications Japan}}, series = {{Microbiology and Immunology}}, title = {{Detection of specific Helicobacter pylori DNA and antigens in stool samples in dyspeptic patients and healthy subjects}}, url = {{http://www.jstage.jst.go.jp/article/mandi/46/10/657/_pdf}}, volume = {{46}}, year = {{2002}}, }