Modulation of postendocytic sorting of G-protein coupled receptors
(2002) In Science 297(5581). p.615-620- Abstract
- Recycling of the mu opioid receptor to the plasma membrane after endocytosis promotes rapid resensitization of signal transduction, whereas targeting of the delta opioid receptor (DOR) to lysosomes causes proteolytic down-regulation. We identified a protein that binds preferentially to the cytoplasmic tail of the DOR as a candidate heterotrimeric GTP-binding protein (G protein)-coupled receptor-associated sorting protein (GASP). Disruption of the DOR-GASP interaction through receptor mutation or overexpression of a dominant negative fragment of GASP inhibited receptor trafficking to lysosomes and promoted recycling. The GASP family of proteins may modulate lysosomal sorting and functional down-regulation of a variety of G protein-coupled... (More)
- Recycling of the mu opioid receptor to the plasma membrane after endocytosis promotes rapid resensitization of signal transduction, whereas targeting of the delta opioid receptor (DOR) to lysosomes causes proteolytic down-regulation. We identified a protein that binds preferentially to the cytoplasmic tail of the DOR as a candidate heterotrimeric GTP-binding protein (G protein)-coupled receptor-associated sorting protein (GASP). Disruption of the DOR-GASP interaction through receptor mutation or overexpression of a dominant negative fragment of GASP inhibited receptor trafficking to lysosomes and promoted recycling. The GASP family of proteins may modulate lysosomal sorting and functional down-regulation of a variety of G protein-coupled receptors. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/333514
- author
- Whistler, JL ; Enquist, Johan LU ; Marley, A ; Fong, J ; Gladher, F ; Tsuruda, P ; Murray, SR and von Zastro, M
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Science
- volume
- 297
- issue
- 5581
- pages
- 615 - 620
- publisher
- American Association for the Advancement of Science (AAAS)
- external identifiers
-
- wos:000177054200055
- pmid:12142540
- scopus:0037178754
- ISSN
- 1095-9203
- language
- English
- LU publication?
- yes
- id
- 03b383aa-66d6-48ba-9283-2ef4df309397 (old id 333514)
- alternative location
- http://stke.sciencemag.org/cgi/reprint/sci;297/5581/615.pdf
- date added to LUP
- 2016-04-01 17:08:52
- date last changed
- 2022-04-15 17:32:34
@article{03b383aa-66d6-48ba-9283-2ef4df309397,
abstract = {{Recycling of the mu opioid receptor to the plasma membrane after endocytosis promotes rapid resensitization of signal transduction, whereas targeting of the delta opioid receptor (DOR) to lysosomes causes proteolytic down-regulation. We identified a protein that binds preferentially to the cytoplasmic tail of the DOR as a candidate heterotrimeric GTP-binding protein (G protein)-coupled receptor-associated sorting protein (GASP). Disruption of the DOR-GASP interaction through receptor mutation or overexpression of a dominant negative fragment of GASP inhibited receptor trafficking to lysosomes and promoted recycling. The GASP family of proteins may modulate lysosomal sorting and functional down-regulation of a variety of G protein-coupled receptors.}},
author = {{Whistler, JL and Enquist, Johan and Marley, A and Fong, J and Gladher, F and Tsuruda, P and Murray, SR and von Zastro, M}},
issn = {{1095-9203}},
language = {{eng}},
number = {{5581}},
pages = {{615--620}},
publisher = {{American Association for the Advancement of Science (AAAS)}},
series = {{Science}},
title = {{Modulation of postendocytic sorting of G-protein coupled receptors}},
url = {{http://stke.sciencemag.org/cgi/reprint/sci;297/5581/615.pdf}},
volume = {{297}},
year = {{2002}},
}