Generating regionalized neuronal cells from pluripotency, a step-by-step protocol.
(2012) In Frontiers in Cellular Neuroscience 6(Published online: 03 January 2013).- Abstract
- Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human... (More)
- Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3438755
- author
- Kirkeby, Agnete LU ; Nelander Wahlestedt, Jenny LU and Parmar, Malin LU
- organization
- publishing date
- 2012
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Frontiers in Cellular Neuroscience
- volume
- 6
- issue
- Published online: 03 January 2013
- article number
- 64
- publisher
- Frontiers Media S. A.
- external identifiers
-
- wos:000312979800001
- pmid:23316134
- scopus:84872186092
- ISSN
- 1662-5102
- DOI
- 10.3389/fncel.2012.00064
- language
- English
- LU publication?
- yes
- id
- 3b917c48-2515-456a-923c-02584939e74c (old id 3438755)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23316134?dopt=Abstract
- date added to LUP
- 2016-04-01 13:45:16
- date last changed
- 2022-04-29 21:59:44
@article{3b917c48-2515-456a-923c-02584939e74c, abstract = {{Human pluripotent stem cells possess the potential to generate cells for regenerative therapies in patients with neurodegenerative diseases, and constitute an excellent cell source for studying human neural development and disease modeling. Protocols for neural differentiation of human pluripotent stem cells have undergone significant progress during recent years, allowing for rapid and synchronized neural conversion. Differentiation procedures can further be combined with accurate and efficient positional patterning to yield regionalized neural progenitors and subtype-specific neurons corresponding to different parts of the developing human brain. Here, we present a step-by-step protocol for neuralization and regionalization of human pluripotent cells for transplantation studies or in vitro analysis.}}, author = {{Kirkeby, Agnete and Nelander Wahlestedt, Jenny and Parmar, Malin}}, issn = {{1662-5102}}, language = {{eng}}, number = {{Published online: 03 January 2013}}, publisher = {{Frontiers Media S. A.}}, series = {{Frontiers in Cellular Neuroscience}}, title = {{Generating regionalized neuronal cells from pluripotency, a step-by-step protocol.}}, url = {{https://lup.lub.lu.se/search/files/3568811/3635660}}, doi = {{10.3389/fncel.2012.00064}}, volume = {{6}}, year = {{2012}}, }