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Evaluation of seven different enzyme-linked immunosorbent assays for serodiagnosis of Staphylococcus aureus bacteremia

Ryding, Ulf LU ; Espersen, F ; Soderquist, B and Christensson, B (2002) In Diagnostic Microbiology and Infectious Disease 42(1). p.9-15
Abstract
Objective: Serologic assays for Staphylococcus aureus antibodies were evaluated regarding their ability to differentiate between uncomplicated and complicated S. aureus bacteremia, between S. aureus and non-S. aureus bacteremia, and between S. aureus and non-S. aureus endocarditis. Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed to measure Ig G antibodies against seven S. altreus antigens (peptidoglycan, teichoic acid, S. aureus ultrasonicate, whole S. aureus cells, a-toxin, lipase and capsular polysaccharide) in 129 patients with S. aureus bacteremia (including 51 with endocarditis), 78 patients with non-S. aureus bacteremia (including 27 with endocarditis) and 100 febrile non-bacteremic controls. Results: Whole-cell... (More)
Objective: Serologic assays for Staphylococcus aureus antibodies were evaluated regarding their ability to differentiate between uncomplicated and complicated S. aureus bacteremia, between S. aureus and non-S. aureus bacteremia, and between S. aureus and non-S. aureus endocarditis. Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed to measure Ig G antibodies against seven S. altreus antigens (peptidoglycan, teichoic acid, S. aureus ultrasonicate, whole S. aureus cells, a-toxin, lipase and capsular polysaccharide) in 129 patients with S. aureus bacteremia (including 51 with endocarditis), 78 patients with non-S. aureus bacteremia (including 27 with endocarditis) and 100 febrile non-bacteremic controls. Results: Whole-cell ELISA was the most sensitive assay. The specificity of all assays was low. Two different combinations of ELISAs for whole cells, teichoic acid, a-toxin, lipase and capsular polysaccharide did distinguish between S. aureus and non-S. aureus endocarditis, but not between uncomplicated and complicated S. aureus bacteremia. (C) 2002 Elsevier Science Inc. All rights reserved. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Diagnostic Microbiology and Infectious Disease
volume
42
issue
1
pages
9 - 15
publisher
Elsevier
external identifiers
  • pmid:11821165
  • wos:000173694900002
  • scopus:0036159479
ISSN
1879-0070
DOI
10.1016/S0732-8893(01)00311-X
language
English
LU publication?
yes
id
e9d30f1b-97a3-45cc-a1cd-f39ff713ba97 (old id 343920)
date added to LUP
2016-04-01 11:33:14
date last changed
2022-01-26 06:58:05
@article{e9d30f1b-97a3-45cc-a1cd-f39ff713ba97,
  abstract     = {{Objective: Serologic assays for Staphylococcus aureus antibodies were evaluated regarding their ability to differentiate between uncomplicated and complicated S. aureus bacteremia, between S. aureus and non-S. aureus bacteremia, and between S. aureus and non-S. aureus endocarditis. Methods: Enzyme-linked immunosorbent assays (ELISAs) were performed to measure Ig G antibodies against seven S. altreus antigens (peptidoglycan, teichoic acid, S. aureus ultrasonicate, whole S. aureus cells, a-toxin, lipase and capsular polysaccharide) in 129 patients with S. aureus bacteremia (including 51 with endocarditis), 78 patients with non-S. aureus bacteremia (including 27 with endocarditis) and 100 febrile non-bacteremic controls. Results: Whole-cell ELISA was the most sensitive assay. The specificity of all assays was low. Two different combinations of ELISAs for whole cells, teichoic acid, a-toxin, lipase and capsular polysaccharide did distinguish between S. aureus and non-S. aureus endocarditis, but not between uncomplicated and complicated S. aureus bacteremia. (C) 2002 Elsevier Science Inc. All rights reserved.}},
  author       = {{Ryding, Ulf and Espersen, F and Soderquist, B and Christensson, B}},
  issn         = {{1879-0070}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{9--15}},
  publisher    = {{Elsevier}},
  series       = {{Diagnostic Microbiology and Infectious Disease}},
  title        = {{Evaluation of seven different enzyme-linked immunosorbent assays for serodiagnosis of Staphylococcus aureus bacteremia}},
  url          = {{http://dx.doi.org/10.1016/S0732-8893(01)00311-X}},
  doi          = {{10.1016/S0732-8893(01)00311-X}},
  volume       = {{42}},
  year         = {{2002}},
}