Identification of a group of nontuberculous mycobacteria isolated from the South Indian BCG trial area by HPLC
(2002) In Current Science 82(2). p.189-191- Abstract
- Twenty-five isolates of nontuberculous mycobacteria isolated from the South Indian BCG trial area were analysed by high performance liquid chromatography (HPLC) for mycolic acid pattern. The chromatograms differentiated the isolates into four species, namely M. terrae complex, M. intracellulare, M. parafortuitum and M. fortuitum. Three strains were unidentified, one of which did not show any mycolic acid peaks. All isolates had been identified as M. diernhoferi by biochemical methods in a previous study. Nineteen of the isolates were analysed by gas chromatography-mass spectrometry (GC-MS) for the presence of tuberculostearic acid, 2-eicosanol and mycolic acid cleavage products, and were classified as nonchromogens or rapidly growing... (More)
- Twenty-five isolates of nontuberculous mycobacteria isolated from the South Indian BCG trial area were analysed by high performance liquid chromatography (HPLC) for mycolic acid pattern. The chromatograms differentiated the isolates into four species, namely M. terrae complex, M. intracellulare, M. parafortuitum and M. fortuitum. Three strains were unidentified, one of which did not show any mycolic acid peaks. All isolates had been identified as M. diernhoferi by biochemical methods in a previous study. Nineteen of the isolates were analysed by gas chromatography-mass spectrometry (GC-MS) for the presence of tuberculostearic acid, 2-eicosanol and mycolic acid cleavage products, and were classified as nonchromogens or rapidly growing mycobacteria. The results show that HPLC can discriminate the described mycobacterial species better than biochemical methods and GC-MS. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/343949
- author
- Vanitha, JD ; Immanuel, C ; Szponar, Bogumila LU ; Larsson, Lennart LU and Paramasivan, CN
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Current Science
- volume
- 82
- issue
- 2
- pages
- 189 - 191
- publisher
- Current Science
- external identifiers
-
- wos:000173671700024
- scopus:0037905344
- ISSN
- 0011-3891
- language
- English
- LU publication?
- yes
- id
- 9c8eadfa-abb3-418b-bed5-e96cd83872de (old id 343949)
- alternative location
- http://www.ias.ac.in/currsci/jan252002/189.pdf
- date added to LUP
- 2016-04-01 17:05:45
- date last changed
- 2022-01-29 00:17:39
@article{9c8eadfa-abb3-418b-bed5-e96cd83872de, abstract = {{Twenty-five isolates of nontuberculous mycobacteria isolated from the South Indian BCG trial area were analysed by high performance liquid chromatography (HPLC) for mycolic acid pattern. The chromatograms differentiated the isolates into four species, namely M. terrae complex, M. intracellulare, M. parafortuitum and M. fortuitum. Three strains were unidentified, one of which did not show any mycolic acid peaks. All isolates had been identified as M. diernhoferi by biochemical methods in a previous study. Nineteen of the isolates were analysed by gas chromatography-mass spectrometry (GC-MS) for the presence of tuberculostearic acid, 2-eicosanol and mycolic acid cleavage products, and were classified as nonchromogens or rapidly growing mycobacteria. The results show that HPLC can discriminate the described mycobacterial species better than biochemical methods and GC-MS.}}, author = {{Vanitha, JD and Immanuel, C and Szponar, Bogumila and Larsson, Lennart and Paramasivan, CN}}, issn = {{0011-3891}}, language = {{eng}}, number = {{2}}, pages = {{189--191}}, publisher = {{Current Science}}, series = {{Current Science}}, title = {{Identification of a group of nontuberculous mycobacteria isolated from the South Indian BCG trial area by HPLC}}, url = {{http://www.ias.ac.in/currsci/jan252002/189.pdf}}, volume = {{82}}, year = {{2002}}, }