Detección de poliovirus en Aguas residuales de Armenia, Colombia
(2006) In Revista de Salud Publica 8(Suppl 1). p.13-23- Abstract
OBJECTIVES: Implementing environmental surveillance for poliovirus circulating from wastewater.
METHODS: Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. Polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and Sabin vaccine-specific primers.
RESULTS: It was confirmed that the viral detection method is highly efficient. Poliovirus was detected in 5 of 6 (83%) samples collected. Serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. All of them... (More)
OBJECTIVES: Implementing environmental surveillance for poliovirus circulating from wastewater.
METHODS: Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. Polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and Sabin vaccine-specific primers.
RESULTS: It was confirmed that the viral detection method is highly efficient. Poliovirus was detected in 5 of 6 (83%) samples collected. Serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. All of them were of the vaccine type, which provided evidence regarding the non-circulation of wild poliovirus in the country.
CONCLUSION: Wastewater surveillance can be a sensitive tool for detecting poliovirus circulation.
(Less)
- author
- González, Maria M ; Sarmiento, Luis LU ; Castaño, Jhon Carlos ; Giraldo, Alejandra M ; Salazar, Alexander and Muñoz, Nini J
- alternative title
- Detecting poliovirus in wastewater from Armenia, Colombia
- publishing date
- 2006-05
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Colombia, Environmental Microbiology, Poliovirus/classification, Poliovirus Vaccine, Oral, Polyethylene Glycols, RNA, Viral/analysis, Reverse Transcriptase Polymerase Chain Reaction, Sewage/virology, Sodium Chloride, Virology/methods
- in
- Revista de Salud Publica
- volume
- 8
- issue
- Suppl 1
- pages
- 11 pages
- publisher
- Universidad Nacional de Colombia
- external identifiers
-
- scopus:33747309124
- pmid:16925118
- ISSN
- 0124-0064
- language
- Spanish
- LU publication?
- no
- id
- 3622d8f3-e0b4-4940-b914-71bdf010a55d
- alternative location
- https://www.scielosp.org/article/rsap/2006.v8suppl1/13-23/es/
- date added to LUP
- 2019-07-08 14:50:53
- date last changed
- 2024-01-01 15:40:07
@article{3622d8f3-e0b4-4940-b914-71bdf010a55d,
abstract = {{<p>OBJECTIVES: Implementing environmental surveillance for poliovirus circulating from wastewater.</p><p>METHODS: Six wastewater samples were collected from each final site within a wastewater collection system in Armenia, Quindío. Virus was extracted and concentrated by a method using polyethylenglycol and sodium chloride as concentrating agent. Polioviruses were identified by polymerase chain reaction assay with group-specific, serotype-specific and Sabin vaccine-specific primers.</p><p>RESULTS: It was confirmed that the viral detection method is highly efficient. Poliovirus was detected in 5 of 6 (83%) samples collected. Serotype-specific identification revealed the presence of type 1 and type 3 polioviruses in the samples. All of them were of the vaccine type, which provided evidence regarding the non-circulation of wild poliovirus in the country.</p><p>CONCLUSION: Wastewater surveillance can be a sensitive tool for detecting poliovirus circulation.</p>}},
author = {{González, Maria M and Sarmiento, Luis and Castaño, Jhon Carlos and Giraldo, Alejandra M and Salazar, Alexander and Muñoz, Nini J}},
issn = {{0124-0064}},
keywords = {{Colombia; Environmental Microbiology; Poliovirus/classification; Poliovirus Vaccine, Oral; Polyethylene Glycols; RNA, Viral/analysis; Reverse Transcriptase Polymerase Chain Reaction; Sewage/virology; Sodium Chloride; Virology/methods}},
language = {{spa}},
number = {{Suppl 1}},
pages = {{13--23}},
publisher = {{Universidad Nacional de Colombia}},
series = {{Revista de Salud Publica}},
title = {{Detección de poliovirus en Aguas residuales de Armenia, Colombia}},
url = {{https://www.scielosp.org/article/rsap/2006.v8suppl1/13-23/es/}},
volume = {{8}},
year = {{2006}},
}