CCD-camera based capillary chemiluminescent detection of retinol binding protein
(1999) In Analytica Chimica Acta 387(1). p.21-27- Abstract
- A chemiluminescence (CL) assay for retinol-binding protein (RBP) was designed and optimized using a charge coupled device (CCD)-camera based detection system. A sandwich ELISA was designed based on the anti-RBP antibodies immobilized in glass capillaries pre-treated with silica sol. The immobilization was predominantly by physisorption of the protein on the silica surface. The RBP bound to the anti-RBP antibodies was detected by using an anti-RBP-horseradish peroxidase (HRP) conjugate. The reaction of the HRP with hydrogen peroxide and luminol and 4-iodophenol generated the CL. The CL emitted from the glass capillaries was detected by a cooled slow scan CCD camera at an optimized exposure time. The approximately linear range of RBP... (More)
- A chemiluminescence (CL) assay for retinol-binding protein (RBP) was designed and optimized using a charge coupled device (CCD)-camera based detection system. A sandwich ELISA was designed based on the anti-RBP antibodies immobilized in glass capillaries pre-treated with silica sol. The immobilization was predominantly by physisorption of the protein on the silica surface. The RBP bound to the anti-RBP antibodies was detected by using an anti-RBP-horseradish peroxidase (HRP) conjugate. The reaction of the HRP with hydrogen peroxide and luminol and 4-iodophenol generated the CL. The CL emitted from the glass capillaries was detected by a cooled slow scan CCD camera at an optimized exposure time. The approximately linear range of RBP determination was between 11 pg ml−1 and 11 ng ml−1 with a coefficient of variation of 5–11% (n=6) over this range. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/365c3d85-8263-4b95-ad34-e7ce4e703488
- author
- Momeni, Naghi LU ; Ramanathan, Kumaran LU ; Larsson, P-O. LU ; Danielsson, Bengt LU ; Bengmark, Stig LU and Khayyami, Masoud
- organization
- publishing date
- 1999
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Chemiluminescence assay, glass capillaries, Horse radish peroxidase
- in
- Analytica Chimica Acta
- volume
- 387
- issue
- 1
- pages
- 7 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0032998072
- ISSN
- 0003-2670
- DOI
- 10.1016/S0003-2670(99)00037-9
- language
- English
- LU publication?
- yes
- id
- 365c3d85-8263-4b95-ad34-e7ce4e703488
- date added to LUP
- 2024-05-18 20:39:02
- date last changed
- 2024-05-30 11:36:36
@article{365c3d85-8263-4b95-ad34-e7ce4e703488, abstract = {{A chemiluminescence (CL) assay for retinol-binding protein (RBP) was designed and optimized using a charge coupled device (CCD)-camera based detection system. A sandwich ELISA was designed based on the anti-RBP antibodies immobilized in glass capillaries pre-treated with silica sol. The immobilization was predominantly by physisorption of the protein on the silica surface. The RBP bound to the anti-RBP antibodies was detected by using an anti-RBP-horseradish peroxidase (HRP) conjugate. The reaction of the HRP with hydrogen peroxide and luminol and 4-iodophenol generated the CL. The CL emitted from the glass capillaries was detected by a cooled slow scan CCD camera at an optimized exposure time. The approximately linear range of RBP determination was between 11 pg ml<sup>−1</sup> and 11 ng ml<sup>−1</sup> with a coefficient of variation of 5–11% (<em>n</em>=6) over this range.}}, author = {{Momeni, Naghi and Ramanathan, Kumaran and Larsson, P-O. and Danielsson, Bengt and Bengmark, Stig and Khayyami, Masoud}}, issn = {{0003-2670}}, keywords = {{Chemiluminescence assay; glass capillaries; Horse radish peroxidase}}, language = {{eng}}, number = {{1}}, pages = {{21--27}}, publisher = {{Elsevier}}, series = {{Analytica Chimica Acta}}, title = {{CCD-camera based capillary chemiluminescent detection of retinol binding protein}}, url = {{http://dx.doi.org/10.1016/S0003-2670(99)00037-9}}, doi = {{10.1016/S0003-2670(99)00037-9}}, volume = {{387}}, year = {{1999}}, }