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CCD-camera based capillary chemiluminescent detection of retinol binding protein

Momeni, Naghi LU ; Ramanathan, Kumaran LU ; Larsson, P-O. LU ; Danielsson, Bengt LU ; Bengmark, Stig LU and Khayyami, Masoud (1999) In Analytica Chimica Acta 387(1). p.21-27
Abstract
A chemiluminescence (CL) assay for retinol-binding protein (RBP) was designed and optimized using a charge coupled device (CCD)-camera based detection system. A sandwich ELISA was designed based on the anti-RBP antibodies immobilized in glass capillaries pre-treated with silica sol. The immobilization was predominantly by physisorption of the protein on the silica surface. The RBP bound to the anti-RBP antibodies was detected by using an anti-RBP-horseradish peroxidase (HRP) conjugate. The reaction of the HRP with hydrogen peroxide and luminol and 4-iodophenol generated the CL. The CL emitted from the glass capillaries was detected by a cooled slow scan CCD camera at an optimized exposure time. The approximately linear range of RBP... (More)
A chemiluminescence (CL) assay for retinol-binding protein (RBP) was designed and optimized using a charge coupled device (CCD)-camera based detection system. A sandwich ELISA was designed based on the anti-RBP antibodies immobilized in glass capillaries pre-treated with silica sol. The immobilization was predominantly by physisorption of the protein on the silica surface. The RBP bound to the anti-RBP antibodies was detected by using an anti-RBP-horseradish peroxidase (HRP) conjugate. The reaction of the HRP with hydrogen peroxide and luminol and 4-iodophenol generated the CL. The CL emitted from the glass capillaries was detected by a cooled slow scan CCD camera at an optimized exposure time. The approximately linear range of RBP determination was between 11 pg ml−1 and 11 ng ml−1 with a coefficient of variation of 5–11% (n=6) over this range. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Chemiluminescence assay, glass capillaries, Horse radish peroxidase
in
Analytica Chimica Acta
volume
387
issue
1
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:0032998072
ISSN
0003-2670
DOI
10.1016/S0003-2670(99)00037-9
language
English
LU publication?
yes
id
365c3d85-8263-4b95-ad34-e7ce4e703488
date added to LUP
2024-05-18 20:39:02
date last changed
2024-05-30 11:36:36
@article{365c3d85-8263-4b95-ad34-e7ce4e703488,
  abstract     = {{A chemiluminescence (CL) assay for retinol-binding protein (RBP) was designed and optimized using a charge coupled device (CCD)-camera based detection system. A sandwich ELISA was designed based on the anti-RBP antibodies immobilized in glass capillaries pre-treated with silica sol. The immobilization was predominantly by physisorption of the protein on the silica surface. The RBP bound to the anti-RBP antibodies was detected by using an anti-RBP-horseradish peroxidase (HRP) conjugate. The reaction of the HRP with hydrogen peroxide and luminol and 4-iodophenol generated the CL. The CL emitted from the glass capillaries was detected by a cooled slow scan CCD camera at an optimized exposure time. The approximately linear range of RBP determination was between 11 pg ml<sup>−1</sup> and 11 ng ml<sup>−1</sup> with a coefficient of variation of 5–11% (<em>n</em>=6) over this range.}},
  author       = {{Momeni, Naghi and Ramanathan, Kumaran and Larsson, P-O. and Danielsson, Bengt and Bengmark, Stig and Khayyami, Masoud}},
  issn         = {{0003-2670}},
  keywords     = {{Chemiluminescence assay; glass capillaries; Horse radish peroxidase}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{21--27}},
  publisher    = {{Elsevier}},
  series       = {{Analytica Chimica Acta}},
  title        = {{CCD-camera based capillary chemiluminescent detection of retinol binding protein}},
  url          = {{http://dx.doi.org/10.1016/S0003-2670(99)00037-9}},
  doi          = {{10.1016/S0003-2670(99)00037-9}},
  volume       = {{387}},
  year         = {{1999}},
}