Fluorescent styrene maleic acid copolymers to facilitate membrane protein studies in lipid nanodiscs
Neville, George M. ; Edler, Karen J. LU
and Price, Gareth J.
(2022)
In Nanoscale
14(15).
p.5689-5693
- Abstract
Fluorescently-labelled variants of poly(styrene-co-maleic acid), SMA, have been synthesised by RAFT copolymerisation. We show that low ratios of vinyl fluorophores, analogous to styrene, can be successfully incorporated during polymerisation without detriment to nanodisc formation upon interaction with lipids. These novel copolymers are capable of encapuslating lipids and the model membrane protein, gramicidin, and hence have the potential to be applied in fluorescence-based biological studies. To demonstrate this, energy transfer is used to probe polymer-protein interactions in nanodiscs. The copolymers may also be used to monitor nanodisc self assembly by exploiting aggregation-caused-quenching (ACQ).
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/36902376-b175-41e9-a40b-ae70bb211b51
- author
- Neville, George M.
; Edler, Karen J.
LU
and Price, Gareth J.
- publishing date
- 2022-03-17
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Nanoscale
- volume
- 14
- issue
- 15
- pages
- 5 pages
- publisher
- Royal Society of Chemistry
- external identifiers
-
- scopus:85127671628
- pmid:35315461
- ISSN
- 2040-3364
- DOI
- 10.1039/d1nr07230g
- language
- English
- LU publication?
- no
- additional info
- Publisher Copyright: © 2022 The Royal Society of Chemistry
- id
- 36902376-b175-41e9-a40b-ae70bb211b51
- date added to LUP
- 2022-07-12 13:58:38
- date last changed
- 2024-06-11 12:46:36
@article{36902376-b175-41e9-a40b-ae70bb211b51,
abstract = {{<p>Fluorescently-labelled variants of poly(styrene-<i>co</i>-maleic acid), SMA, have been synthesised by RAFT copolymerisation. We show that low ratios of vinyl fluorophores, analogous to styrene, can be successfully incorporated during polymerisation without detriment to nanodisc formation upon interaction with lipids. These novel copolymers are capable of encapuslating lipids and the model membrane protein, gramicidin, and hence have the potential to be applied in fluorescence-based biological studies. To demonstrate this, energy transfer is used to probe polymer-protein interactions in nanodiscs. The copolymers may also be used to monitor nanodisc self assembly by exploiting aggregation-caused-quenching (ACQ).</p>}},
author = {{Neville, George M. and Edler, Karen J. and Price, Gareth J.}},
issn = {{2040-3364}},
language = {{eng}},
month = {{03}},
number = {{15}},
pages = {{5689--5693}},
publisher = {{Royal Society of Chemistry}},
series = {{Nanoscale}},
title = {{Fluorescent styrene maleic acid copolymers to facilitate membrane protein studies in lipid nanodiscs}},
url = {{http://dx.doi.org/10.1039/d1nr07230g}},
doi = {{10.1039/d1nr07230g}},
volume = {{14}},
year = {{2022}},
}