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Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family

Aapola, U ; Shibuya, K ; Scott, HS ; Ollila, J ; Vihinen, Mauno LU orcid ; Heino, M ; Shintani, A ; Kawasaki, K ; Minoshima, S and Krohn, K , et al. (2000) In Genomics 65(3). p.293-298
Abstract
We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family, The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including... (More)
We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family, The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. (C) 2000 Academic Press. (Less)
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publishing date
type
Contribution to journal
publication status
published
subject
in
Genomics
volume
65
issue
3
pages
293 - 298
publisher
Academic Press
external identifiers
  • wos:000087127500011
  • scopus:18744427037
ISSN
1089-8646
DOI
10.1006/geno.2000.6168
language
English
LU publication?
no
id
44690037-d1a7-4549-8119-fc2ba83a7c84 (old id 3852086)
date added to LUP
2016-04-01 12:00:07
date last changed
2022-04-21 00:58:10
@article{44690037-d1a7-4549-8119-fc2ba83a7c84,
  abstract     = {{We have isolated the DNMT3L gene that is related to the cytosine-5-methyltransferase 3 (DNMT3) family. The gene is located on chromosome 21q22.3 between the AIRE and the KIAA0653 genes and spans approximately 16 kb of genomic sequence. The encoded protein of 387 amino acids has a cysteine-rich region containing a novel-type zinc finger domain that is conserved in DNMT3A and DNMT3B but also in ATRX, a member of the SNF2 protein family, The novel domain, called an ADD (ATRX, DNMT3, DNMT3L)-type zinc finger, contains two subparts: a C2C2 and an imperfect PHD zinc finger. Expression of the DNMT3L mRNA was not detectable by Northern blotting; however, RT-PCR amplification revealed that it is expressed at low levels in several tissues including testis, ovary, and thymus. (C) 2000 Academic Press.}},
  author       = {{Aapola, U and Shibuya, K and Scott, HS and Ollila, J and Vihinen, Mauno and Heino, M and Shintani, A and Kawasaki, K and Minoshima, S and Krohn, K and Antonarakis, SE and Shimizu, N and Kudoh, J and Peterson, P}},
  issn         = {{1089-8646}},
  language     = {{eng}},
  number       = {{3}},
  pages        = {{293--298}},
  publisher    = {{Academic Press}},
  series       = {{Genomics}},
  title        = {{Isolation and initial characterization of a novel zinc finger gene, DNMT3L, on 21q22.3, related to the cytosine-5-methyltransferase 3 gene family}},
  url          = {{http://dx.doi.org/10.1006/geno.2000.6168}},
  doi          = {{10.1006/geno.2000.6168}},
  volume       = {{65}},
  year         = {{2000}},
}