Diffusion of lysozyme in gels and liquids - A general approach for the determination of diffusion coefficients using holographic laser interferometry
(2000) In Journal of Chromatography. B 743(1-2). p.151-167- Abstract
- A study on diffusion measurements of the protein lysozyme in liquids and agarose gels, at different pH and ionic strengths, has been performed using holographic laser interferometry. The measurements showed that the diffusive flux was very dependent on pH and ionic strength when the protein was not at its isoelectric point or when the charge of the lysozyme molecules was not screened by ions in the solution. Evaluation of the experimental data with Fick's law, resulted in diffusion coefficients for lysozyme that are strongly dependent on pH and ionic strength. Evaluation of the experimental data using a more general transport model, based on chemical potential gradients instead of concentration gradients resulted in lysozyme diffusion... (More)
- A study on diffusion measurements of the protein lysozyme in liquids and agarose gels, at different pH and ionic strengths, has been performed using holographic laser interferometry. The measurements showed that the diffusive flux was very dependent on pH and ionic strength when the protein was not at its isoelectric point or when the charge of the lysozyme molecules was not screened by ions in the solution. Evaluation of the experimental data with Fick's law, resulted in diffusion coefficients for lysozyme that are strongly dependent on pH and ionic strength. Evaluation of the experimental data using a more general transport model, based on chemical potential gradients instead of concentration gradients resulted in lysozyme diffusion coefficients that are independent of pH and ionic strength. The chemical potential was estimated by using the Poisson-Boltzmann equation. (C) 2000 Elsevier Science B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3911339
- author
- Mattisson, C ; Roger, P ; Jönsson, Bengt LU ; Axelsson, Anders LU and Zacchi, Guido LU
- organization
- publishing date
- 2000
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- diffusion coefficients, holographic laser interferometry, lysozome
- in
- Journal of Chromatography. B
- volume
- 743
- issue
- 1-2
- pages
- 151 - 167
- publisher
- Elsevier
- external identifiers
-
- wos:000088042000018
- scopus:0034705682
- ISSN
- 1387-2273
- DOI
- 10.1016/S0378-4347(99)00535-6
- language
- English
- LU publication?
- yes
- id
- 9f1fa24f-3837-4008-9105-f5f915824dcc (old id 3911339)
- date added to LUP
- 2016-04-01 15:18:07
- date last changed
- 2023-11-13 16:58:12
@article{9f1fa24f-3837-4008-9105-f5f915824dcc,
abstract = {{A study on diffusion measurements of the protein lysozyme in liquids and agarose gels, at different pH and ionic strengths, has been performed using holographic laser interferometry. The measurements showed that the diffusive flux was very dependent on pH and ionic strength when the protein was not at its isoelectric point or when the charge of the lysozyme molecules was not screened by ions in the solution. Evaluation of the experimental data with Fick's law, resulted in diffusion coefficients for lysozyme that are strongly dependent on pH and ionic strength. Evaluation of the experimental data using a more general transport model, based on chemical potential gradients instead of concentration gradients resulted in lysozyme diffusion coefficients that are independent of pH and ionic strength. The chemical potential was estimated by using the Poisson-Boltzmann equation. (C) 2000 Elsevier Science B.V. All rights reserved.}},
author = {{Mattisson, C and Roger, P and Jönsson, Bengt and Axelsson, Anders and Zacchi, Guido}},
issn = {{1387-2273}},
keywords = {{diffusion coefficients; holographic laser interferometry; lysozome}},
language = {{eng}},
number = {{1-2}},
pages = {{151--167}},
publisher = {{Elsevier}},
series = {{Journal of Chromatography. B}},
title = {{Diffusion of lysozyme in gels and liquids - A general approach for the determination of diffusion coefficients using holographic laser interferometry}},
url = {{http://dx.doi.org/10.1016/S0378-4347(99)00535-6}},
doi = {{10.1016/S0378-4347(99)00535-6}},
volume = {{743}},
year = {{2000}},
}