Cellulase production by T-reesei
(1996) In Bioresource Technology 57(1). p.25-30- Abstract
- Steam-pretreated willow has been found to be a suitable substrate for ethanol production in Sweden. The production of Trichoderma reesei cellulases on steam-pretreated willow (SPW) of varying cellulose content has been studied. Batch fermentations were performed in shake flasks, and in 4- and 22-l fermenters. SPW was found to be a suitable carbon source for cellulase production. At an initial solids concentration corresponding to 10 g/l cellulose, the yield was 108 filter paper units (FPU)/g cellulose after 3 days in the 22-l fermenter However, the yield was below 100 FPU/g cellulose in the shake flasks and 4-l fermenter The solid residue of enzymatically hydrolysed SPW was found to be nearly as good a substrate as SPW, although the... (More)
- Steam-pretreated willow has been found to be a suitable substrate for ethanol production in Sweden. The production of Trichoderma reesei cellulases on steam-pretreated willow (SPW) of varying cellulose content has been studied. Batch fermentations were performed in shake flasks, and in 4- and 22-l fermenters. SPW was found to be a suitable carbon source for cellulase production. At an initial solids concentration corresponding to 10 g/l cellulose, the yield was 108 filter paper units (FPU)/g cellulose after 3 days in the 22-l fermenter However, the yield was below 100 FPU/g cellulose in the shake flasks and 4-l fermenter The solid residue of enzymatically hydrolysed SPW was found to be nearly as good a substrate as SPW, although the cellulose content was as low as 20% in this hydrolysis residue. Using delignified SPW, the yield of cellulases was low, indicating that lignin does not inhibit cellulase production. By applying a pressure of 1.7 bar in the fermenter, the initial fermentation rate was increased. The productivity was increased from 12.2 FPU/l/h to 16.7 FPU/l/h, due to the higher level of dissolved oxygen at the higher pressure. Copyright (C) 1996 Elsevier Science Ltd. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3911525
- author
- Reczey, K ; Szengyel, Z ; Eklund, R and Zacchi, Guido LU
- organization
- publishing date
- 1996
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- enzyme production, cellulases, Trichoderma reesei, lignocellulose, steam, pretreatment
- in
- Bioresource Technology
- volume
- 57
- issue
- 1
- pages
- 25 - 30
- publisher
- Elsevier
- external identifiers
-
- wos:A1996VP46600004
- scopus:0030200061
- ISSN
- 1873-2976
- DOI
- 10.1016/0960-8524(96)00038-7
- language
- English
- LU publication?
- yes
- id
- b1076648-bdd0-49fc-a739-81efb4d9ddd1 (old id 3911525)
- date added to LUP
- 2016-04-01 15:26:24
- date last changed
- 2023-11-13 18:36:21
@article{b1076648-bdd0-49fc-a739-81efb4d9ddd1,
abstract = {{Steam-pretreated willow has been found to be a suitable substrate for ethanol production in Sweden. The production of Trichoderma reesei cellulases on steam-pretreated willow (SPW) of varying cellulose content has been studied. Batch fermentations were performed in shake flasks, and in 4- and 22-l fermenters. SPW was found to be a suitable carbon source for cellulase production. At an initial solids concentration corresponding to 10 g/l cellulose, the yield was 108 filter paper units (FPU)/g cellulose after 3 days in the 22-l fermenter However, the yield was below 100 FPU/g cellulose in the shake flasks and 4-l fermenter The solid residue of enzymatically hydrolysed SPW was found to be nearly as good a substrate as SPW, although the cellulose content was as low as 20% in this hydrolysis residue. Using delignified SPW, the yield of cellulases was low, indicating that lignin does not inhibit cellulase production. By applying a pressure of 1.7 bar in the fermenter, the initial fermentation rate was increased. The productivity was increased from 12.2 FPU/l/h to 16.7 FPU/l/h, due to the higher level of dissolved oxygen at the higher pressure. Copyright (C) 1996 Elsevier Science Ltd.}},
author = {{Reczey, K and Szengyel, Z and Eklund, R and Zacchi, Guido}},
issn = {{1873-2976}},
keywords = {{enzyme production; cellulases; Trichoderma reesei; lignocellulose; steam; pretreatment}},
language = {{eng}},
number = {{1}},
pages = {{25--30}},
publisher = {{Elsevier}},
series = {{Bioresource Technology}},
title = {{Cellulase production by T-reesei}},
url = {{http://dx.doi.org/10.1016/0960-8524(96)00038-7}},
doi = {{10.1016/0960-8524(96)00038-7}},
volume = {{57}},
year = {{1996}},
}