C2028T polymorphism in exon 12 and dinucleotide repeat polymorphism in intron 13 of the HIF-1 alpha gene define HIF-1 alpha protein expression in non-small cell lung cancer
(2006) In Lung Cancer 53(3). p.257-262- Abstract
- Objectives: In this study, we investigated whether polymorphisms of the HIF-1 alpha gene may account for the patterns of HIF-1 alpha protein expression in non-small cell lung carcinomas (NSCLC) and the expression of HIF-1 alpha down-stream proteins. Methods: Specific HIF-1 alpha polymorphisms were assessed in a series of patients with NSCLC: (a) the C to T transition at nucleotide 1744 (position 2028 according to sequence with accession number NM_001530, which gives rise to Pro/Ser variation at codon 582), (b) the G to A nucleotide substitution at point 1790 (position 2046 according to sequence with accession number NM_001530, which gives rise to Ala/Thr variation at codon 588), and (c) the dinucleotide GT repeat polymorphism in intron 13.... (More)
- Objectives: In this study, we investigated whether polymorphisms of the HIF-1 alpha gene may account for the patterns of HIF-1 alpha protein expression in non-small cell lung carcinomas (NSCLC) and the expression of HIF-1 alpha down-stream proteins. Methods: Specific HIF-1 alpha polymorphisms were assessed in a series of patients with NSCLC: (a) the C to T transition at nucleotide 1744 (position 2028 according to sequence with accession number NM_001530, which gives rise to Pro/Ser variation at codon 582), (b) the G to A nucleotide substitution at point 1790 (position 2046 according to sequence with accession number NM_001530, which gives rise to Ala/Thr variation at codon 588), and (c) the dinucleotide GT repeat polymorphism in intron 13. Immunohistochemistry for HIF-1 alpha and down-stream proteins (VEGF, LDH-5, GLUT-1) was also performed in tumor material. Results: A strong association of the P582S polymorphism and of GT repeat polymorphism higher than 14/14 with increased HIF-1 alpha expression was noted. HIF-1 alpha polymorphism did not relate to the expression of the HIF-1 alpha downstream proteins analysed, but significant association of HIF-1 alpha expression with LDH-5 was confirmed (p=0.008). Conclusions: HIF-1 alpha polymorphisms may have an important impact on HIF-protein stability and, eventually, function. (C) 2006 Elsevier Ireland Ltd. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/394696
- author
- Koukourakis, Michael I. ; Papazoglou, Dimitrios ; Giatromanolaki, Alexandra ; Panagopoulos, Ioannis LU ; Maltezos, Efstratios ; Harris, Adrian L. ; Gatter, Kevin C. and Sivridis, Efthimios
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- expression, HIF-1 alpha gene, polymorphism, lung cancer
- in
- Lung Cancer
- volume
- 53
- issue
- 3
- pages
- 257 - 262
- publisher
- Elsevier
- external identifiers
-
- wos:000240422500001
- scopus:33747061025
- pmid:16837101
- ISSN
- 1872-8332
- DOI
- 10.1016/j.lungcan.2006.05.025
- language
- English
- LU publication?
- yes
- id
- 64888835-c2ef-4918-b3c7-aed0a4c11782 (old id 394696)
- date added to LUP
- 2016-04-01 12:15:36
- date last changed
- 2022-04-21 04:58:42
@article{64888835-c2ef-4918-b3c7-aed0a4c11782,
abstract = {{Objectives: In this study, we investigated whether polymorphisms of the HIF-1 alpha gene may account for the patterns of HIF-1 alpha protein expression in non-small cell lung carcinomas (NSCLC) and the expression of HIF-1 alpha down-stream proteins. Methods: Specific HIF-1 alpha polymorphisms were assessed in a series of patients with NSCLC: (a) the C to T transition at nucleotide 1744 (position 2028 according to sequence with accession number NM_001530, which gives rise to Pro/Ser variation at codon 582), (b) the G to A nucleotide substitution at point 1790 (position 2046 according to sequence with accession number NM_001530, which gives rise to Ala/Thr variation at codon 588), and (c) the dinucleotide GT repeat polymorphism in intron 13. Immunohistochemistry for HIF-1 alpha and down-stream proteins (VEGF, LDH-5, GLUT-1) was also performed in tumor material. Results: A strong association of the P582S polymorphism and of GT repeat polymorphism higher than 14/14 with increased HIF-1 alpha expression was noted. HIF-1 alpha polymorphism did not relate to the expression of the HIF-1 alpha downstream proteins analysed, but significant association of HIF-1 alpha expression with LDH-5 was confirmed (p=0.008). Conclusions: HIF-1 alpha polymorphisms may have an important impact on HIF-protein stability and, eventually, function. (C) 2006 Elsevier Ireland Ltd. All rights reserved.}},
author = {{Koukourakis, Michael I. and Papazoglou, Dimitrios and Giatromanolaki, Alexandra and Panagopoulos, Ioannis and Maltezos, Efstratios and Harris, Adrian L. and Gatter, Kevin C. and Sivridis, Efthimios}},
issn = {{1872-8332}},
keywords = {{expression; HIF-1 alpha gene; polymorphism; lung cancer}},
language = {{eng}},
number = {{3}},
pages = {{257--262}},
publisher = {{Elsevier}},
series = {{Lung Cancer}},
title = {{C2028T polymorphism in exon 12 and dinucleotide repeat polymorphism in intron 13 of the HIF-1 alpha gene define HIF-1 alpha protein expression in non-small cell lung cancer}},
url = {{http://dx.doi.org/10.1016/j.lungcan.2006.05.025}},
doi = {{10.1016/j.lungcan.2006.05.025}},
volume = {{53}},
year = {{2006}},
}