Leukemia associated mutant Wilms' tumor gene 1 protein promotes expansion of human hematopoietic progenitor cells.
(2013) In Leukemia Research: A Forum for Studies on Leukemia and Normal Hemopoiesis 37(10). p.1341-1349- Abstract
- The transcription factor Wilms' tumor gene 1 (WT1) is highly expressed in the majority of leukemias, suggesting a role in leukemogenesis. Acquired WT1 mutations are reported as an independent predictor of poor clinical outcome, and mutations resulting in deletion of the entire DNA-binding zinc-finger domain (WT1delZ), is the most common type. The aim of this study was to study cellular effects of WT1(delZ) that may contribute to an oncogenic phenotype. We found that expression of WT1(delZ) supported proliferation of human hematopoietic CD34(+) progenitor cells. Moreover, WT1(delZ) transduced cells expressed erythroid markers, including raised levels of STAT5, independently of addition of erythropoietin. At the global gene expression level,... (More)
- The transcription factor Wilms' tumor gene 1 (WT1) is highly expressed in the majority of leukemias, suggesting a role in leukemogenesis. Acquired WT1 mutations are reported as an independent predictor of poor clinical outcome, and mutations resulting in deletion of the entire DNA-binding zinc-finger domain (WT1delZ), is the most common type. The aim of this study was to study cellular effects of WT1(delZ) that may contribute to an oncogenic phenotype. We found that expression of WT1(delZ) supported proliferation of human hematopoietic CD34(+) progenitor cells. Moreover, WT1(delZ) transduced cells expressed erythroid markers, including raised levels of STAT5, independently of addition of erythropoietin. At the global gene expression level, WT1(delZ) caused upregulation of genes related to cell division and genes associated with erythroid maturation, in the absence of added erythropoietin. Our results indicate that WT1(delZ) promotes cell proliferation and expansion of progenitor cells, consistent with a possible role in leukemogenesis. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3955732
- author
- Vidovic, Karina LU ; Ullmark, Tove LU ; Rosberg, Bodil LU ; Lennartsson, Andreas LU ; Olofsson, Tor LU ; Nilsson, Björn LU and Gullberg, Urban LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Leukemia Research: A Forum for Studies on Leukemia and Normal Hemopoiesis
- volume
- 37
- issue
- 10
- pages
- 1341 - 1349
- publisher
- Elsevier
- external identifiers
-
- wos:000324099100028
- pmid:23871158
- scopus:84883557198
- pmid:23871158
- ISSN
- 1873-5835
- DOI
- 10.1016/j.leukres.2013.06.018
- language
- English
- LU publication?
- yes
- id
- ada5eb33-e2b4-42f6-8b07-684dc7c14767 (old id 3955732)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/23871158?dopt=Abstract
- date added to LUP
- 2016-04-01 09:54:52
- date last changed
- 2024-10-06 15:45:36
@article{ada5eb33-e2b4-42f6-8b07-684dc7c14767, abstract = {{The transcription factor Wilms' tumor gene 1 (WT1) is highly expressed in the majority of leukemias, suggesting a role in leukemogenesis. Acquired WT1 mutations are reported as an independent predictor of poor clinical outcome, and mutations resulting in deletion of the entire DNA-binding zinc-finger domain (WT1delZ), is the most common type. The aim of this study was to study cellular effects of WT1(delZ) that may contribute to an oncogenic phenotype. We found that expression of WT1(delZ) supported proliferation of human hematopoietic CD34(+) progenitor cells. Moreover, WT1(delZ) transduced cells expressed erythroid markers, including raised levels of STAT5, independently of addition of erythropoietin. At the global gene expression level, WT1(delZ) caused upregulation of genes related to cell division and genes associated with erythroid maturation, in the absence of added erythropoietin. Our results indicate that WT1(delZ) promotes cell proliferation and expansion of progenitor cells, consistent with a possible role in leukemogenesis.}}, author = {{Vidovic, Karina and Ullmark, Tove and Rosberg, Bodil and Lennartsson, Andreas and Olofsson, Tor and Nilsson, Björn and Gullberg, Urban}}, issn = {{1873-5835}}, language = {{eng}}, number = {{10}}, pages = {{1341--1349}}, publisher = {{Elsevier}}, series = {{Leukemia Research: A Forum for Studies on Leukemia and Normal Hemopoiesis}}, title = {{Leukemia associated mutant Wilms' tumor gene 1 protein promotes expansion of human hematopoietic progenitor cells.}}, url = {{https://lup.lub.lu.se/search/files/1383913/4173655.pdf}}, doi = {{10.1016/j.leukres.2013.06.018}}, volume = {{37}}, year = {{2013}}, }