Seasonal carbon allocation to arbuscular mycorrhizal fungi assessed by microscopic examination, stable isotope probing and fatty acid analysis
(2013) In Plant and Soil 368(1-2). p.547-555- Abstract
- Background and Aim Climate change models are limited by lack of baseline data, in particular carbon (C) allocation to - and dynamics within - soil microbial communities. We quantified seasonal C-assimilation and allocation by plants, and assessed how well this corresponds with intraradical arbuscular mycorrhizal fungal (AMF) storage and structural lipids (16: 1 omega 5 NLFA and PLFA, respectively), as well as microscopic assessments of AMF root colonization. Methods Coastal Hypochoeris radicata plants were labeled with (CO2)-C-13 in February, July and October, and C-13-allocation to fine roots and NLFA 16: 1 omega 5, as well as overall lipid contents and AM colonization were quantified. Results C-allocation to fine roots and AMF storage... (More)
- Background and Aim Climate change models are limited by lack of baseline data, in particular carbon (C) allocation to - and dynamics within - soil microbial communities. We quantified seasonal C-assimilation and allocation by plants, and assessed how well this corresponds with intraradical arbuscular mycorrhizal fungal (AMF) storage and structural lipids (16: 1 omega 5 NLFA and PLFA, respectively), as well as microscopic assessments of AMF root colonization. Methods Coastal Hypochoeris radicata plants were labeled with (CO2)-C-13 in February, July and October, and C-13-allocation to fine roots and NLFA 16: 1 omega 5, as well as overall lipid contents and AM colonization were quantified. Results C-allocation to fine roots and AMF storage lipids differed seasonally and mirrored plant C-assimilation, whereas AMF structural lipids and AM colonization showed no seasonal variation, and root colonization exceeded 80 % throughout the year. Molecular analyzes of the large subunit rDNA gene indicated no seasonal AMF community shifts. Conclusions Plants allocated C to AMF even at temperatures close to freezing, and fungal structures persisted in roots during times of low C-allocation. The lack of seasonal differences in PLFA and AM colonization indicates that NLFA analyses should be used to estimate fungal C-status. The implication of our findings for AM function is discussed. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/3974096
- author
- Lekberg, Ylva ; Rosendahl, Soren ; Michelsen, Anders and Olsson, Pål Axel LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Arbuscular mycorrhiza, Carbon allocation, Grassland, Lipid analysis, Season, Stable isotope probing
- in
- Plant and Soil
- volume
- 368
- issue
- 1-2
- pages
- 547 - 555
- publisher
- Springer
- external identifiers
-
- wos:000321641700041
- scopus:84879170765
- ISSN
- 0032-079X
- DOI
- 10.1007/s11104-012-1534-7
- language
- English
- LU publication?
- yes
- id
- 01751022-e616-493a-a3a8-904bc26bf901 (old id 3974096)
- date added to LUP
- 2016-04-01 13:25:22
- date last changed
- 2024-04-10 05:34:04
@article{01751022-e616-493a-a3a8-904bc26bf901, abstract = {{Background and Aim Climate change models are limited by lack of baseline data, in particular carbon (C) allocation to - and dynamics within - soil microbial communities. We quantified seasonal C-assimilation and allocation by plants, and assessed how well this corresponds with intraradical arbuscular mycorrhizal fungal (AMF) storage and structural lipids (16: 1 omega 5 NLFA and PLFA, respectively), as well as microscopic assessments of AMF root colonization. Methods Coastal Hypochoeris radicata plants were labeled with (CO2)-C-13 in February, July and October, and C-13-allocation to fine roots and NLFA 16: 1 omega 5, as well as overall lipid contents and AM colonization were quantified. Results C-allocation to fine roots and AMF storage lipids differed seasonally and mirrored plant C-assimilation, whereas AMF structural lipids and AM colonization showed no seasonal variation, and root colonization exceeded 80 % throughout the year. Molecular analyzes of the large subunit rDNA gene indicated no seasonal AMF community shifts. Conclusions Plants allocated C to AMF even at temperatures close to freezing, and fungal structures persisted in roots during times of low C-allocation. The lack of seasonal differences in PLFA and AM colonization indicates that NLFA analyses should be used to estimate fungal C-status. The implication of our findings for AM function is discussed.}}, author = {{Lekberg, Ylva and Rosendahl, Soren and Michelsen, Anders and Olsson, Pål Axel}}, issn = {{0032-079X}}, keywords = {{Arbuscular mycorrhiza; Carbon allocation; Grassland; Lipid analysis; Season; Stable isotope probing}}, language = {{eng}}, number = {{1-2}}, pages = {{547--555}}, publisher = {{Springer}}, series = {{Plant and Soil}}, title = {{Seasonal carbon allocation to arbuscular mycorrhizal fungi assessed by microscopic examination, stable isotope probing and fatty acid analysis}}, url = {{http://dx.doi.org/10.1007/s11104-012-1534-7}}, doi = {{10.1007/s11104-012-1534-7}}, volume = {{368}}, year = {{2013}}, }