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Small-angle X-ray and neutron scattering applied to lipid-based nanoparticles : Recent advancements across different length scales

Caselli, Lucrezia LU ; Conti, Laura ; De Santis, Ilaria and Berti, Debora (2024) In Advances in Colloid and Interface Science 327.
Abstract

Lipid-based nanoparticles (LNPs), ranging from nanovesicles to non-lamellar assemblies, have gained significant attention in recent years, as versatile carriers for delivering drugs, vaccines, and nutrients. Small-angle scattering methods, employing X-rays (SAXS) or neutrons (SANS), represent unique tools to unveil structure, dynamics, and interactions of such particles on different length scales, spanning from the nano to the molecular scale. This review explores the state-of-the-art on scattering methods applied to unveil the structure of lipid-based nanoparticles and their interactions with drugs and bioactive molecules, to inform their rational design and formulation for medical applications. We will focus on complementary... (More)

Lipid-based nanoparticles (LNPs), ranging from nanovesicles to non-lamellar assemblies, have gained significant attention in recent years, as versatile carriers for delivering drugs, vaccines, and nutrients. Small-angle scattering methods, employing X-rays (SAXS) or neutrons (SANS), represent unique tools to unveil structure, dynamics, and interactions of such particles on different length scales, spanning from the nano to the molecular scale. This review explores the state-of-the-art on scattering methods applied to unveil the structure of lipid-based nanoparticles and their interactions with drugs and bioactive molecules, to inform their rational design and formulation for medical applications. We will focus on complementary information accessible with X-rays or neutrons, ranging from insights on the structure and colloidal processes at a nanoscale level (SAXS) to details on the lipid organization and molecular interactions of LNPs (SANS). In addition, we will review new opportunities offered by Time-resolved (TR)-SAXS and -SANS for the investigation of dynamic processes involving LNPs. These span from real-time monitoring of LNPs structural evolution in response to endogenous or external stimuli (TR-SANS), to the investigation of the kinetics of lipid diffusion and exchange upon interaction with biomolecules (TR-SANS). Finally, we will spotlight novel combinations of SAXS and SANS with complementary on-line techniques, recently enabled at Large Scale Facilities for X-rays and neutrons. This emerging technology enables synchronized multi-method investigation, offering exciting opportunities for the simultaneous characterization of the structure and chemical or mechanical properties of LNPs.

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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Cubosomes, Lipid nanoparticles, Lipid vesicles, Small-angle neutron scattering, Small-angle X-ray scattering
in
Advances in Colloid and Interface Science
volume
327
article number
103156
publisher
Elsevier
external identifiers
  • pmid:38643519
  • scopus:85190737828
ISSN
0001-8686
DOI
10.1016/j.cis.2024.103156
language
English
LU publication?
yes
id
399b57fd-2d9e-49c0-8176-fe6f7a14116b
date added to LUP
2024-04-29 12:27:35
date last changed
2024-10-29 07:05:18
@article{399b57fd-2d9e-49c0-8176-fe6f7a14116b,
  abstract     = {{<p>Lipid-based nanoparticles (LNPs), ranging from nanovesicles to non-lamellar assemblies, have gained significant attention in recent years, as versatile carriers for delivering drugs, vaccines, and nutrients. Small-angle scattering methods, employing X-rays (SAXS) or neutrons (SANS), represent unique tools to unveil structure, dynamics, and interactions of such particles on different length scales, spanning from the nano to the molecular scale. This review explores the state-of-the-art on scattering methods applied to unveil the structure of lipid-based nanoparticles and their interactions with drugs and bioactive molecules, to inform their rational design and formulation for medical applications. We will focus on complementary information accessible with X-rays or neutrons, ranging from insights on the structure and colloidal processes at a nanoscale level (SAXS) to details on the lipid organization and molecular interactions of LNPs (SANS). In addition, we will review new opportunities offered by Time-resolved (TR)-SAXS and -SANS for the investigation of dynamic processes involving LNPs. These span from real-time monitoring of LNPs structural evolution in response to endogenous or external stimuli (TR-SANS), to the investigation of the kinetics of lipid diffusion and exchange upon interaction with biomolecules (TR-SANS). Finally, we will spotlight novel combinations of SAXS and SANS with complementary on-line techniques, recently enabled at Large Scale Facilities for X-rays and neutrons. This emerging technology enables synchronized multi-method investigation, offering exciting opportunities for the simultaneous characterization of the structure and chemical or mechanical properties of LNPs.</p>}},
  author       = {{Caselli, Lucrezia and Conti, Laura and De Santis, Ilaria and Berti, Debora}},
  issn         = {{0001-8686}},
  keywords     = {{Cubosomes; Lipid nanoparticles; Lipid vesicles; Small-angle neutron scattering; Small-angle X-ray scattering}},
  language     = {{eng}},
  publisher    = {{Elsevier}},
  series       = {{Advances in Colloid and Interface Science}},
  title        = {{Small-angle X-ray and neutron scattering applied to lipid-based nanoparticles : Recent advancements across different length scales}},
  url          = {{http://dx.doi.org/10.1016/j.cis.2024.103156}},
  doi          = {{10.1016/j.cis.2024.103156}},
  volume       = {{327}},
  year         = {{2024}},
}