Characterization of the genome, proteome, and structure of yersiniophage φR1-37
(2012) In Journal of Virology 86(23). p.12625-12642- Abstract
The bacteriophage vB_YecM-φR1-37 (φR1-37) is a lytic yersiniophage that can propagate naturally in different Yersinia species carrying the correct lipopolysaccharide receptor. This large-tailed phage has deoxyuridine (dU) instead of thymidine in its DNA. In this study, we determined the genomic sequence of phage φR1-37, mapped parts of the phage transcriptome, characterized the phage particle proteome, and characterized the virion structure by cryo-electron microscopy and image reconstruction. The 262,391-bp genome of φR1-37 is one of the largest sequenced phage genomes, and it contains 367 putative open reading frames (ORFs) and 5 tRNA genes. Mass-spectrometric analysis identified 69 phage particle structural proteins with the genes... (More)
The bacteriophage vB_YecM-φR1-37 (φR1-37) is a lytic yersiniophage that can propagate naturally in different Yersinia species carrying the correct lipopolysaccharide receptor. This large-tailed phage has deoxyuridine (dU) instead of thymidine in its DNA. In this study, we determined the genomic sequence of phage φR1-37, mapped parts of the phage transcriptome, characterized the phage particle proteome, and characterized the virion structure by cryo-electron microscopy and image reconstruction. The 262,391-bp genome of φR1-37 is one of the largest sequenced phage genomes, and it contains 367 putative open reading frames (ORFs) and 5 tRNA genes. Mass-spectrometric analysis identified 69 phage particle structural proteins with the genes scattered throughout the genome. A total of 269 of the ORFs (73%) lack homologues in sequence databases. Based on terminator and promoter sequences identified from the intergenic regions, the phage genome was predicted to consist of 40 to 60 transcriptional units. Image reconstruction revealed that the φR1-37 capsid consists of hexameric capsomers arranged on a T=27 lattice similar to the bacteriophage φKZ. The tail of φR1-37 has a contractile sheath. We conclude that phage φR1-37 is a representative of a novel phage type that carries the dU-containing genome in a φKZ-like head.
(Less)
- author
- organization
- publishing date
- 2012-12
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Virology
- volume
- 86
- issue
- 23
- pages
- 18 pages
- publisher
- American Society for Microbiology
- external identifiers
-
- scopus:84869216595
- pmid:22973030
- ISSN
- 0022-538X
- DOI
- 10.1128/JVI.01783-12
- language
- English
- LU publication?
- yes
- additional info
- Copyright 2013 Elsevier B.V., All rights reserved. Copyright © 2012, American Society for Microbiology
- id
- 3cd2b664-0bb8-4472-9d52-15b10b0e1c62
- date added to LUP
- 2021-03-22 09:58:02
- date last changed
- 2024-03-08 10:44:32
@article{3cd2b664-0bb8-4472-9d52-15b10b0e1c62, abstract = {{<p>The bacteriophage vB_YecM-φR1-37 (φR1-37) is a lytic yersiniophage that can propagate naturally in different Yersinia species carrying the correct lipopolysaccharide receptor. This large-tailed phage has deoxyuridine (dU) instead of thymidine in its DNA. In this study, we determined the genomic sequence of phage φR1-37, mapped parts of the phage transcriptome, characterized the phage particle proteome, and characterized the virion structure by cryo-electron microscopy and image reconstruction. The 262,391-bp genome of φR1-37 is one of the largest sequenced phage genomes, and it contains 367 putative open reading frames (ORFs) and 5 tRNA genes. Mass-spectrometric analysis identified 69 phage particle structural proteins with the genes scattered throughout the genome. A total of 269 of the ORFs (73%) lack homologues in sequence databases. Based on terminator and promoter sequences identified from the intergenic regions, the phage genome was predicted to consist of 40 to 60 transcriptional units. Image reconstruction revealed that the φR1-37 capsid consists of hexameric capsomers arranged on a T=27 lattice similar to the bacteriophage φKZ. The tail of φR1-37 has a contractile sheath. We conclude that phage φR1-37 is a representative of a novel phage type that carries the dU-containing genome in a φKZ-like head.</p>}}, author = {{Skurnik, Mikael and Hyytiäinen, Heidi J. and Happonen, Lotta J. and Kiljunen, Saija and Datta, Neeta and Mattinen, Laura and Williamson, Kirsty and Kristo, Paula and Szeliga, Magdalena and Kalin-Mänttäri, Laura and Ahola-Iivarinen, Elina and Kalkkinen, Nisse and Butcher, Sarah J.}}, issn = {{0022-538X}}, language = {{eng}}, number = {{23}}, pages = {{12625--12642}}, publisher = {{American Society for Microbiology}}, series = {{Journal of Virology}}, title = {{Characterization of the genome, proteome, and structure of yersiniophage φR1-37}}, url = {{http://dx.doi.org/10.1128/JVI.01783-12}}, doi = {{10.1128/JVI.01783-12}}, volume = {{86}}, year = {{2012}}, }