Spontaneous in vitro immunoglobulin secretion at the diagnosis of insulin-dependent diabetes
(1984) In Acta Endocrinologica 105(4). p.521-527- Abstract
Blood mononuclear cells obtained from 17 newly diagnosed insulin-dependent diabetic (IDDM) patients treated with insulin for 5-7 days were assessed for the number of spontaneous and pokeweed mitogen (PWM)-stimulated immunoglobulin-secreting cells in a reverse haemolytic plaque assay. The spontaneous in vitro immunoglobulin secretion was evanescent and decreased in individual patients within 1-4 months of insulin treatment. Compared to matched controls, 53% (9/17) of the IDDM patients had an elevated spontaneous secretion of immunoglobulin, 41% (7/17) for IgG, 35% (6/17) for IgM, and 35% (6/17) for IgA. The quantities of PWM-stimulated IgG, IgM, or IgA secreting cells in IDDM were comparable to the controls. The IDDM patients with... (More)
Blood mononuclear cells obtained from 17 newly diagnosed insulin-dependent diabetic (IDDM) patients treated with insulin for 5-7 days were assessed for the number of spontaneous and pokeweed mitogen (PWM)-stimulated immunoglobulin-secreting cells in a reverse haemolytic plaque assay. The spontaneous in vitro immunoglobulin secretion was evanescent and decreased in individual patients within 1-4 months of insulin treatment. Compared to matched controls, 53% (9/17) of the IDDM patients had an elevated spontaneous secretion of immunoglobulin, 41% (7/17) for IgG, 35% (6/17) for IgM, and 35% (6/17) for IgA. The quantities of PWM-stimulated IgG, IgM, or IgA secreting cells in IDDM were comparable to the controls. The IDDM patients with spontaneous immunoglobulin secreting cells had higher fasting C-peptide levels compared to the patients with immunoglobulin-producing cells within the normal range (P < 0.05). The average titre of islet cell cytoplasmic antibodies was 1:26 in (9 out of 9 were positive) patients with, compared to 1:1 in patients (4 out of 8 were positive) without spontaneous secretion (P = 0.025). These results suggest that the clinical onset of IDDM is associated with a polyclonal B lymphocyte activation and that higher levels of fasting C-peptide islet cell antibodies are associated with this immunoregulatory abnormality.
(Less)
- author
- Papadopoulos, G. ; Petersen, J. ; Andersen, V. ; Lernmark, A. LU ; Marner, B. ; Nerup, J. LU and Binder, C.
- publishing date
- 1984-05-24
- type
- Contribution to journal
- publication status
- published
- in
- Acta Endocrinologica
- volume
- 105
- issue
- 4
- pages
- 7 pages
- publisher
- Society of the European Journal of Endocrinology
- external identifiers
-
- pmid:6372349
- scopus:0021325119
- ISSN
- 0001-5598
- language
- English
- LU publication?
- no
- id
- 3fc67cf8-66c8-4536-ac37-736b0bbcffa9
- date added to LUP
- 2019-09-16 12:42:06
- date last changed
- 2024-03-13 08:00:10
@article{3fc67cf8-66c8-4536-ac37-736b0bbcffa9, abstract = {{<p>Blood mononuclear cells obtained from 17 newly diagnosed insulin-dependent diabetic (IDDM) patients treated with insulin for 5-7 days were assessed for the number of spontaneous and pokeweed mitogen (PWM)-stimulated immunoglobulin-secreting cells in a reverse haemolytic plaque assay. The spontaneous in vitro immunoglobulin secretion was evanescent and decreased in individual patients within 1-4 months of insulin treatment. Compared to matched controls, 53% (9/17) of the IDDM patients had an elevated spontaneous secretion of immunoglobulin, 41% (7/17) for IgG, 35% (6/17) for IgM, and 35% (6/17) for IgA. The quantities of PWM-stimulated IgG, IgM, or IgA secreting cells in IDDM were comparable to the controls. The IDDM patients with spontaneous immunoglobulin secreting cells had higher fasting C-peptide levels compared to the patients with immunoglobulin-producing cells within the normal range (P < 0.05). The average titre of islet cell cytoplasmic antibodies was 1:26 in (9 out of 9 were positive) patients with, compared to 1:1 in patients (4 out of 8 were positive) without spontaneous secretion (P = 0.025). These results suggest that the clinical onset of IDDM is associated with a polyclonal B lymphocyte activation and that higher levels of fasting C-peptide islet cell antibodies are associated with this immunoregulatory abnormality.</p>}}, author = {{Papadopoulos, G. and Petersen, J. and Andersen, V. and Lernmark, A. and Marner, B. and Nerup, J. and Binder, C.}}, issn = {{0001-5598}}, language = {{eng}}, month = {{05}}, number = {{4}}, pages = {{521--527}}, publisher = {{Society of the European Journal of Endocrinology}}, series = {{Acta Endocrinologica}}, title = {{Spontaneous in vitro immunoglobulin secretion at the diagnosis of insulin-dependent diabetes}}, volume = {{105}}, year = {{1984}}, }