A novel thermometric biosensor for fast surveillance of beta-lactamase activity in milk
(2013) In Biosensors & Bioelectronics 49. p.99-104- Abstract
- Regulatory restrictions on antibiotic residues in dairy products have resulted in the illegal addition of beta-lactamase to lower antibiotic levels in milk in China. Here we demonstrate a fast, sensitive and convenient method based on enzyme thermistor (ET) for the surveillance of beta-lactamase in milk. A fixed amount of penicillin G, which is a specific substrate of beta-lactamase, was incubated with the milk sample, and an aliquot of the mixture was directly injected into the ET system to give a temperature change corresponding to the remained penicillin G. The amount of beta-lactamase present in sample was deduced by the penicillin G consumed during incubation. This method was successfully applied to quantify beta-lactamase in milk... (More)
- Regulatory restrictions on antibiotic residues in dairy products have resulted in the illegal addition of beta-lactamase to lower antibiotic levels in milk in China. Here we demonstrate a fast, sensitive and convenient method based on enzyme thermistor (ET) for the surveillance of beta-lactamase in milk. A fixed amount of penicillin G, which is a specific substrate of beta-lactamase, was incubated with the milk sample, and an aliquot of the mixture was directly injected into the ET system to give a temperature change corresponding to the remained penicillin G. The amount of beta-lactamase present in sample was deduced by the penicillin G consumed during incubation. This method was successfully applied to quantify beta-lactamase in milk with the linear range of 1.1-20 U mL(-1) and the detection limit of 1.1 U mL(-1). The recoveries ranged from 93% to 105%, with relative standard deviations (RSDs) below 8%. The stability of the column equipped in ET was also studied, and only 5% decrease of activity was observed after 60 days of use. Compared with the conventional culture-based assay, the advantages of high throughput, timesaving and accurate quantification have made this method an ideal alternative for routine use. (C) 2013 Elsevier B.V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4025558
- author
- Zhou, Shuang ; Zhao, Yunfeng ; Mecklenburg, Michael ; Yang, Dajin and Xie, Bin LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- beta-lactamase, Antibiotics, Milk, Enzyme thermistor, Enzyme, immobilization
- in
- Biosensors & Bioelectronics
- volume
- 49
- pages
- 99 - 104
- publisher
- Elsevier
- external identifiers
-
- wos:000323396700015
- scopus:84878418180
- pmid:23722048
- ISSN
- 1873-4235
- DOI
- 10.1016/j.bios.2013.05.005
- language
- English
- LU publication?
- yes
- id
- b5507ca3-b02a-4f99-bb20-0d24ec580ea8 (old id 4025558)
- date added to LUP
- 2016-04-01 14:36:09
- date last changed
- 2022-01-28 01:30:27
@article{b5507ca3-b02a-4f99-bb20-0d24ec580ea8,
abstract = {{Regulatory restrictions on antibiotic residues in dairy products have resulted in the illegal addition of beta-lactamase to lower antibiotic levels in milk in China. Here we demonstrate a fast, sensitive and convenient method based on enzyme thermistor (ET) for the surveillance of beta-lactamase in milk. A fixed amount of penicillin G, which is a specific substrate of beta-lactamase, was incubated with the milk sample, and an aliquot of the mixture was directly injected into the ET system to give a temperature change corresponding to the remained penicillin G. The amount of beta-lactamase present in sample was deduced by the penicillin G consumed during incubation. This method was successfully applied to quantify beta-lactamase in milk with the linear range of 1.1-20 U mL(-1) and the detection limit of 1.1 U mL(-1). The recoveries ranged from 93% to 105%, with relative standard deviations (RSDs) below 8%. The stability of the column equipped in ET was also studied, and only 5% decrease of activity was observed after 60 days of use. Compared with the conventional culture-based assay, the advantages of high throughput, timesaving and accurate quantification have made this method an ideal alternative for routine use. (C) 2013 Elsevier B.V. All rights reserved.}},
author = {{Zhou, Shuang and Zhao, Yunfeng and Mecklenburg, Michael and Yang, Dajin and Xie, Bin}},
issn = {{1873-4235}},
keywords = {{beta-lactamase; Antibiotics; Milk; Enzyme thermistor; Enzyme; immobilization}},
language = {{eng}},
pages = {{99--104}},
publisher = {{Elsevier}},
series = {{Biosensors & Bioelectronics}},
title = {{A novel thermometric biosensor for fast surveillance of beta-lactamase activity in milk}},
url = {{http://dx.doi.org/10.1016/j.bios.2013.05.005}},
doi = {{10.1016/j.bios.2013.05.005}},
volume = {{49}},
year = {{2013}},
}