Formate dehydrogenase-modified carbon paste electrodes for amperometric detection of formate
(2006) In Revue Roumaine de Chimie 51(1). p.25-30- Abstract
- A simple and inexpensive amperometric biosensor for formate detection was obtained by immobilization of for-mate dehydrogenase (FDH) on graphite powder, modified with a new phenothiazine derivative (DDDP; 16H, 18H-dibenzo[c,l]-7,9-dithia-16,18-diazapentacene). Two different enzyme environments were used: FDH was adsorbed on DDDP-modified graphite powder in the presence of polyethylenimine (PEI) as well as in its absence. In both cases, when 10 mM NAD(+) were added in the supporting electrolyte (phosphate buffer, pH 7.0), the biosensor developed a response to formate, obeying Michaelis-Menten kinetics with a linear domain up to 3 mM formate. The biosensor sensitivity, calculated as the ratio 1(max)/K-M(aPP), was better in the second case... (More)
- A simple and inexpensive amperometric biosensor for formate detection was obtained by immobilization of for-mate dehydrogenase (FDH) on graphite powder, modified with a new phenothiazine derivative (DDDP; 16H, 18H-dibenzo[c,l]-7,9-dithia-16,18-diazapentacene). Two different enzyme environments were used: FDH was adsorbed on DDDP-modified graphite powder in the presence of polyethylenimine (PEI) as well as in its absence. In both cases, when 10 mM NAD(+) were added in the supporting electrolyte (phosphate buffer, pH 7.0), the biosensor developed a response to formate, obeying Michaelis-Menten kinetics with a linear domain up to 3 mM formate. The biosensor sensitivity, calculated as the ratio 1(max)/K-M(aPP), was better in the second case (0.962 mA/M), but the best detection limit (3 AM) was observed in the first case. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/405825
- author
- Munteanu, Florentina LU ; Gligor, D ; Popescu, IC and Gorton, Lo LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Revue Roumaine de Chimie
- volume
- 51
- issue
- 1
- pages
- 25 - 30
- publisher
- Romanian Academy / Academia Române
- external identifiers
-
- wos:000238234900004
- scopus:33745014881
- ISSN
- 0035-3930
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Analytical Chemistry (S/LTH) (011001004)
- id
- 2b7bcf0a-d1ab-44c8-9fa0-85d8f8a86350 (old id 405825)
- date added to LUP
- 2016-04-01 16:23:05
- date last changed
- 2022-01-28 19:17:49
@article{2b7bcf0a-d1ab-44c8-9fa0-85d8f8a86350, abstract = {{A simple and inexpensive amperometric biosensor for formate detection was obtained by immobilization of for-mate dehydrogenase (FDH) on graphite powder, modified with a new phenothiazine derivative (DDDP; 16H, 18H-dibenzo[c,l]-7,9-dithia-16,18-diazapentacene). Two different enzyme environments were used: FDH was adsorbed on DDDP-modified graphite powder in the presence of polyethylenimine (PEI) as well as in its absence. In both cases, when 10 mM NAD(+) were added in the supporting electrolyte (phosphate buffer, pH 7.0), the biosensor developed a response to formate, obeying Michaelis-Menten kinetics with a linear domain up to 3 mM formate. The biosensor sensitivity, calculated as the ratio 1(max)/K-M(aPP), was better in the second case (0.962 mA/M), but the best detection limit (3 AM) was observed in the first case.}}, author = {{Munteanu, Florentina and Gligor, D and Popescu, IC and Gorton, Lo}}, issn = {{0035-3930}}, language = {{eng}}, number = {{1}}, pages = {{25--30}}, publisher = {{Romanian Academy / Academia Române}}, series = {{Revue Roumaine de Chimie}}, title = {{Formate dehydrogenase-modified carbon paste electrodes for amperometric detection of formate}}, volume = {{51}}, year = {{2006}}, }