Characterization of insulin microcrystals using powder diffraction and multivariate data analysis
(2006) In Journal of Applied Crystallography 39. p.391-400- Abstract
- Twelve different microcrystalline insulin formulations were investigated by X-ray powder diffraction and were shown to have very characteristic patterns. Three of the formulations crystallize in the same crystal system, but have structural differences in the N-terminal B-chain of the insulin molecule. This difference was efficiently detected in the powder patterns. The sensitivity of the method makes it a valuable tool for characterization of microcrystalline samples. By use of principal-component analysis, the twelve different formulations originating from six different crystal systems were classified into nine separate clusters. The powder patterns of each cluster can now be used as `fingerprints' for the different insulin polymorphs.... (More)
- Twelve different microcrystalline insulin formulations were investigated by X-ray powder diffraction and were shown to have very characteristic patterns. Three of the formulations crystallize in the same crystal system, but have structural differences in the N-terminal B-chain of the insulin molecule. This difference was efficiently detected in the powder patterns. The sensitivity of the method makes it a valuable tool for characterization of microcrystalline samples. By use of principal-component analysis, the twelve different formulations originating from six different crystal systems were classified into nine separate clusters. The powder patterns of each cluster can now be used as `fingerprints' for the different insulin polymorphs. The combination of X-ray powder diffraction and multivariate analysis, such as principal-component analysis, provides a rapid and effective tool for studying the influence of derivatives, additives, ions, pH etc., in the crystallization media. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/409518
- author
- Norrman, Mathias LU ; Stahl, K ; Schluckebier, G and Al-Karadaghi, Salam LU
- organization
- publishing date
- 2006
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Applied Crystallography
- volume
- 39
- pages
- 391 - 400
- publisher
- International Union of Crystallography
- external identifiers
-
- wos:000237461400012
- scopus:33744454406
- ISSN
- 1600-5767
- DOI
- 10.1107/S0021889806011058
- language
- English
- LU publication?
- yes
- id
- 50dba17a-cbc2-42a7-b57a-3ca069d08480 (old id 409518)
- date added to LUP
- 2016-04-01 12:38:47
- date last changed
- 2022-01-27 07:55:57
@article{50dba17a-cbc2-42a7-b57a-3ca069d08480,
abstract = {{Twelve different microcrystalline insulin formulations were investigated by X-ray powder diffraction and were shown to have very characteristic patterns. Three of the formulations crystallize in the same crystal system, but have structural differences in the N-terminal B-chain of the insulin molecule. This difference was efficiently detected in the powder patterns. The sensitivity of the method makes it a valuable tool for characterization of microcrystalline samples. By use of principal-component analysis, the twelve different formulations originating from six different crystal systems were classified into nine separate clusters. The powder patterns of each cluster can now be used as `fingerprints' for the different insulin polymorphs. The combination of X-ray powder diffraction and multivariate analysis, such as principal-component analysis, provides a rapid and effective tool for studying the influence of derivatives, additives, ions, pH etc., in the crystallization media.}},
author = {{Norrman, Mathias and Stahl, K and Schluckebier, G and Al-Karadaghi, Salam}},
issn = {{1600-5767}},
language = {{eng}},
pages = {{391--400}},
publisher = {{International Union of Crystallography}},
series = {{Journal of Applied Crystallography}},
title = {{Characterization of insulin microcrystals using powder diffraction and multivariate data analysis}},
url = {{http://dx.doi.org/10.1107/S0021889806011058}},
doi = {{10.1107/S0021889806011058}},
volume = {{39}},
year = {{2006}},
}