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Modified ZymoBIOMICS™ DNA/RNA Miniprep Kit for parallel extraction of DNA and RNA from peat soil

Mondav, Rhiannon LU orcid (2025)
Abstract
Modified ZYMO DNA/RNA miniprep extraction protocol written in a user friendly way to reduce in-process-decisions and process time. Sample type is organic soil, peat, from a degraded rich fen

This protocol includes modifications to tackle clogged filters.

Samples were centrifuged for 45 seconds instead of 30 seconds in first pass through filters as 100 ul to 200 ul sample remained trapped above filter with standard protocol.
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Working paper/Preprint
publication status
published
subject
publisher
protocols.io
DOI
10.17504/protocols.io.ewov1167ovr2/v1
project
Impact of rewetting drained peatlands on soil microbial activity and composition and its climate mitigation potential
language
English
LU publication?
yes
id
40c79318-50da-46fa-be05-69d85e330691
date added to LUP
2025-12-02 08:20:18
date last changed
2025-12-05 16:01:00
@misc{40c79318-50da-46fa-be05-69d85e330691,
  abstract     = {{Modified ZYMO DNA/RNA miniprep extraction protocol written in a user friendly way to reduce in-process-decisions and process time. Sample type is organic soil, peat, from a degraded rich fen<br/><br/>This protocol includes modifications to tackle clogged filters. <br/><br/>Samples were centrifuged for 45 seconds instead of 30 seconds in first pass through filters as 100 ul to 200 ul sample remained trapped above filter with standard protocol.}},
  author       = {{Mondav, Rhiannon}},
  language     = {{eng}},
  month        = {{11}},
  note         = {{Preprint}},
  publisher    = {{protocols.io}},
  title        = {{Modified ZymoBIOMICS™ DNA/RNA Miniprep Kit for parallel extraction of DNA and RNA from peat soil}},
  url          = {{http://dx.doi.org/10.17504/protocols.io.ewov1167ovr2/v1}},
  doi          = {{10.17504/protocols.io.ewov1167ovr2/v1}},
  year         = {{2025}},
}