The Na(+) transport in Gram-positive bacteria defect in the Mrp antiporter complex measured with (23)Na-NMR.
(2014) In Analytical Biochemistry 445(Online 15 October 2013). p.80-86- Abstract
- (23)Na-NMR has previously been used to monitor Na(+) translocation across membranes in Gram-negative bacteria and in various other organelles and liposomes using a membrane-impermeable shift reagent to resolve the signals resulting from internal and external Na(+). In this work, the (23)Na-NMR method was adapted for measurements of internal Na(+) concentration in the Gram-positive bacterium Bacillus subtilis, with the aim of assessing the Na(+) translocation activity of the Mrp antiporter complex, a member of the Cation Proton Antiporter-3 (CPA-3) family. The sodium sensitive growth phenotype observed in a B. subtilis strain with the gene encoding MrpA deleted, could indeed be correlated to the inability of this strain to maintain a lower... (More)
- (23)Na-NMR has previously been used to monitor Na(+) translocation across membranes in Gram-negative bacteria and in various other organelles and liposomes using a membrane-impermeable shift reagent to resolve the signals resulting from internal and external Na(+). In this work, the (23)Na-NMR method was adapted for measurements of internal Na(+) concentration in the Gram-positive bacterium Bacillus subtilis, with the aim of assessing the Na(+) translocation activity of the Mrp antiporter complex, a member of the Cation Proton Antiporter-3 (CPA-3) family. The sodium sensitive growth phenotype observed in a B. subtilis strain with the gene encoding MrpA deleted, could indeed be correlated to the inability of this strain to maintain a lower internal than external Na(+) concentration. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4143164
- author
- Gorecki, Kamil LU ; Hägerhäll, Cecilia LU and Drakenberg, Torbjörn LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Analytical Biochemistry
- volume
- 445
- issue
- Online 15 October 2013
- pages
- 80 - 86
- publisher
- Elsevier
- external identifiers
-
- wos:000328236800013
- pmid:24139955
- scopus:84888116310
- pmid:24139955
- ISSN
- 1096-0309
- DOI
- 10.1016/j.ab.2013.10.003
- language
- English
- LU publication?
- yes
- id
- 02d06514-0cde-46d3-adcb-b0fc71d6a03a (old id 4143164)
- date added to LUP
- 2016-04-01 11:07:50
- date last changed
- 2022-02-03 00:04:25
@article{02d06514-0cde-46d3-adcb-b0fc71d6a03a, abstract = {{(23)Na-NMR has previously been used to monitor Na(+) translocation across membranes in Gram-negative bacteria and in various other organelles and liposomes using a membrane-impermeable shift reagent to resolve the signals resulting from internal and external Na(+). In this work, the (23)Na-NMR method was adapted for measurements of internal Na(+) concentration in the Gram-positive bacterium Bacillus subtilis, with the aim of assessing the Na(+) translocation activity of the Mrp antiporter complex, a member of the Cation Proton Antiporter-3 (CPA-3) family. The sodium sensitive growth phenotype observed in a B. subtilis strain with the gene encoding MrpA deleted, could indeed be correlated to the inability of this strain to maintain a lower internal than external Na(+) concentration.}}, author = {{Gorecki, Kamil and Hägerhäll, Cecilia and Drakenberg, Torbjörn}}, issn = {{1096-0309}}, language = {{eng}}, number = {{Online 15 October 2013}}, pages = {{80--86}}, publisher = {{Elsevier}}, series = {{Analytical Biochemistry}}, title = {{The Na(+) transport in Gram-positive bacteria defect in the Mrp antiporter complex measured with (23)Na-NMR.}}, url = {{http://dx.doi.org/10.1016/j.ab.2013.10.003}}, doi = {{10.1016/j.ab.2013.10.003}}, volume = {{445}}, year = {{2014}}, }