Analysis of Mice Lacking the Heparin-Binding Splice Isoform of Platelet-Derived Growth Factor A
Andrae, Johanna ; Ehrencrona, Hans LU
; Gallini, Radiosa
; Lal, Mark
; Ding, Hao
and Betsholtz, Christer
(2013)
In Molecular and Cellular Biology
33(20).
p.4030-4040
- Abstract
- Platelet-derived growth factor A-chain (PDGF-A) exists in two evolutionarily conserved isoforms, PDGF-Along and PDGF-Ashort, generated by alternative RNA splicing. They differ by the presence (in PDGF-Along) or absence (in PDGF-Ashort) of a carboxyterminal heparin/heparan sulfate proteoglycan-binding motif. In mice, similar motifs present in other members of the PDGF and vascular endothelial growth factor (VEGF) families have been functionally analyzed in vivo, but the specific physiological importance of PDGF-A(long) has not been explored previously. Here, we analyzed the absolute and relative expression of the two PDGF-A splice isoforms during early postnatal organ development in the mouse and report on the generation of a Pdgfa allele... (More)
- Platelet-derived growth factor A-chain (PDGF-A) exists in two evolutionarily conserved isoforms, PDGF-Along and PDGF-Ashort, generated by alternative RNA splicing. They differ by the presence (in PDGF-Along) or absence (in PDGF-Ashort) of a carboxyterminal heparin/heparan sulfate proteoglycan-binding motif. In mice, similar motifs present in other members of the PDGF and vascular endothelial growth factor (VEGF) families have been functionally analyzed in vivo, but the specific physiological importance of PDGF-A(long) has not been explored previously. Here, we analyzed the absolute and relative expression of the two PDGF-A splice isoforms during early postnatal organ development in the mouse and report on the generation of a Pdgfa allele (Pdgfa(Delta ex6) incapable of producing PDGF-A(long) due to a deletion of the exon 6 splice acceptor site. In situations of limiting PDGF-A signaling through PDGF receptor alpha (PDGFR alpha), or in mice lacking PDGF-C, homozygous carriers of Pdgfa(Delta ex6) showed abnormal development of the lung, intestine, and vertebral column, pinpointing developmental processes where PDGF-A(long) may play a physiological role. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4163478
- author
- Andrae, Johanna
; Ehrencrona, Hans
LU
; Gallini, Radiosa
; Lal, Mark
; Ding, Hao
and Betsholtz, Christer
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Molecular and Cellular Biology
- volume
- 33
- issue
- 20
- pages
- 4030 - 4040
- publisher
- American Society for Microbiology
- external identifiers
-
- wos:000324912000007
- scopus:84886922238
- pmid:23938297
- ISSN
- 0270-7306
- DOI
- 10.1128/MCB.00749-13
- language
- English
- LU publication?
- yes
- id
- d3c8ca6e-a260-4793-8573-57aba2a1125b (old id 4163478)
- date added to LUP
- 2016-04-01 10:05:08
- date last changed
- 2022-02-24 22:05:26
@article{d3c8ca6e-a260-4793-8573-57aba2a1125b,
abstract = {{Platelet-derived growth factor A-chain (PDGF-A) exists in two evolutionarily conserved isoforms, PDGF-Along and PDGF-Ashort, generated by alternative RNA splicing. They differ by the presence (in PDGF-Along) or absence (in PDGF-Ashort) of a carboxyterminal heparin/heparan sulfate proteoglycan-binding motif. In mice, similar motifs present in other members of the PDGF and vascular endothelial growth factor (VEGF) families have been functionally analyzed in vivo, but the specific physiological importance of PDGF-A(long) has not been explored previously. Here, we analyzed the absolute and relative expression of the two PDGF-A splice isoforms during early postnatal organ development in the mouse and report on the generation of a Pdgfa allele (Pdgfa(Delta ex6) incapable of producing PDGF-A(long) due to a deletion of the exon 6 splice acceptor site. In situations of limiting PDGF-A signaling through PDGF receptor alpha (PDGFR alpha), or in mice lacking PDGF-C, homozygous carriers of Pdgfa(Delta ex6) showed abnormal development of the lung, intestine, and vertebral column, pinpointing developmental processes where PDGF-A(long) may play a physiological role.}},
author = {{Andrae, Johanna and Ehrencrona, Hans and Gallini, Radiosa and Lal, Mark and Ding, Hao and Betsholtz, Christer}},
issn = {{0270-7306}},
language = {{eng}},
number = {{20}},
pages = {{4030--4040}},
publisher = {{American Society for Microbiology}},
series = {{Molecular and Cellular Biology}},
title = {{Analysis of Mice Lacking the Heparin-Binding Splice Isoform of Platelet-Derived Growth Factor A}},
url = {{http://dx.doi.org/10.1128/MCB.00749-13}},
doi = {{10.1128/MCB.00749-13}},
volume = {{33}},
year = {{2013}},
}