Automated flow-injection immunosensor based on current pulse capacitive measurements
(2014) In Sensors and Actuators B: Chemical 190. p.295-304- Abstract
- This document describes a new concept for assessing capacitance based on a constant current pulse to the biosensor transducer. The biosensor has a working electrode that is coated with an insulating molecular layer including a ligand which forms an affinity surface. A sensor electrode is brought into contact with electrolyte solution, and the new measuring principle then involves steps where three different constant currents (I-1, I-2 and I-3) are serially pulsed on the sensor surface during pre-determined time periods. The potential that is built up (rising) across the sensor surface is sampled every 6.8 mu s. The inclination of the registered potential profile corresponding to the current pulsed was utilized to calculate both capacitance... (More)
- This document describes a new concept for assessing capacitance based on a constant current pulse to the biosensor transducer. The biosensor has a working electrode that is coated with an insulating molecular layer including a ligand which forms an affinity surface. A sensor electrode is brought into contact with electrolyte solution, and the new measuring principle then involves steps where three different constant currents (I-1, I-2 and I-3) are serially pulsed on the sensor surface during pre-determined time periods. The potential that is built up (rising) across the sensor surface is sampled every 6.8 mu s. The inclination of the registered potential profile corresponding to the current pulsed was utilized to calculate both capacitance and resistance. The new current-based measurement method shows a 10-fold increase in stability for the capacitive measurement as compared to the potential pulse technique. Quantitation of HIV-1 p24 using monoclonal anti-HIV-1 p24 antibodies was used as a model system for the evaluation of the technique. The binding of HIV-1 p24 antigens to the immobilized antibodies causes the capacitance to decrease. The change in capacitance was proportional to the concentration of HIV-1 p24. The capacitance measurement using the current pulse method offers a stable sensing technique with a broad range of potential applications. (C) 2013 Elsevier B. V. All rights reserved. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4197876
- author
- Erlandsson, Dag ; Teeparuksapun, Kosin LU ; Mattiasson, Bo LU and Hedström, Martin LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Current pulse, Capacitive, Automated flow-injection, HIV-1 p24
- in
- Sensors and Actuators B: Chemical
- volume
- 190
- pages
- 295 - 304
- publisher
- Elsevier
- external identifiers
-
- wos:000326687700039
- scopus:84884678128
- ISSN
- 0925-4005
- DOI
- 10.1016/j.snb.2013.08.076
- language
- English
- LU publication?
- yes
- id
- f86869f3-427f-401d-b82a-319e7ff95fd2 (old id 4197876)
- date added to LUP
- 2016-04-01 15:00:22
- date last changed
- 2022-03-29 23:53:35
@article{f86869f3-427f-401d-b82a-319e7ff95fd2,
abstract = {{This document describes a new concept for assessing capacitance based on a constant current pulse to the biosensor transducer. The biosensor has a working electrode that is coated with an insulating molecular layer including a ligand which forms an affinity surface. A sensor electrode is brought into contact with electrolyte solution, and the new measuring principle then involves steps where three different constant currents (I-1, I-2 and I-3) are serially pulsed on the sensor surface during pre-determined time periods. The potential that is built up (rising) across the sensor surface is sampled every 6.8 mu s. The inclination of the registered potential profile corresponding to the current pulsed was utilized to calculate both capacitance and resistance. The new current-based measurement method shows a 10-fold increase in stability for the capacitive measurement as compared to the potential pulse technique. Quantitation of HIV-1 p24 using monoclonal anti-HIV-1 p24 antibodies was used as a model system for the evaluation of the technique. The binding of HIV-1 p24 antigens to the immobilized antibodies causes the capacitance to decrease. The change in capacitance was proportional to the concentration of HIV-1 p24. The capacitance measurement using the current pulse method offers a stable sensing technique with a broad range of potential applications. (C) 2013 Elsevier B. V. All rights reserved.}},
author = {{Erlandsson, Dag and Teeparuksapun, Kosin and Mattiasson, Bo and Hedström, Martin}},
issn = {{0925-4005}},
keywords = {{Current pulse; Capacitive; Automated flow-injection; HIV-1 p24}},
language = {{eng}},
pages = {{295--304}},
publisher = {{Elsevier}},
series = {{Sensors and Actuators B: Chemical}},
title = {{Automated flow-injection immunosensor based on current pulse capacitive measurements}},
url = {{http://dx.doi.org/10.1016/j.snb.2013.08.076}},
doi = {{10.1016/j.snb.2013.08.076}},
volume = {{190}},
year = {{2014}},
}