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Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor

Uzuncayir, Sibel LU ; Vera-Rodriguez, Arturo ; Regenthal, Paulina LU ; Åbacka, Hannah LU orcid ; Emanuelsson, Cecilia LU orcid ; Bahl, Christopher D. and Lindkvist-Petersson, Karin LU (2022) In FEBS Letters 596(7). p.910-923
Abstract
Superantigens (SAgs) are bacterial enterotoxins produced by Staphylococcus aureus. Staphylococcal enterotoxin type A (SEA), a staphylococcal superantigen, has been shown to bind to the cytokine signalling receptor glycoprotein 130 (gp130). The structural details, as well as the exact physiological role of this interaction, remain unclear. Here, we describe the structural details of the SEA–gp130 complex by combining crosslinking mass spectrometry and computational modelling. Interestingly, SEA is not able to bind gp130-homologues from rat and mouse. Our data suggest that SEA may interact with human gp130 in a different manner than other known gp130-ligands. Moreover, the fact that SEA does not bind mouse or rat gp130 suggests that SAgs... (More)
Superantigens (SAgs) are bacterial enterotoxins produced by Staphylococcus aureus. Staphylococcal enterotoxin type A (SEA), a staphylococcal superantigen, has been shown to bind to the cytokine signalling receptor glycoprotein 130 (gp130). The structural details, as well as the exact physiological role of this interaction, remain unclear. Here, we describe the structural details of the SEA–gp130 complex by combining crosslinking mass spectrometry and computational modelling. Interestingly, SEA is not able to bind gp130-homologues from rat and mouse. Our data suggest that SEA may interact with human gp130 in a different manner than other known gp130-ligands. Moreover, the fact that SEA does not bind mouse or rat gp130 suggests that SAgs have additional mechanisms of action in humans. (Less)
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author
; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
FEBS Letters
volume
596
issue
7
pages
910 - 923
publisher
Wiley-Blackwell
external identifiers
  • pmid:35060124
  • scopus:85123885785
ISSN
1873-3468
DOI
10.1002/1873-3468.14292
language
English
LU publication?
yes
id
426d5761-c245-452a-9de1-a8e558af10d2
date added to LUP
2022-04-07 10:43:47
date last changed
2023-05-15 10:44:06
@article{426d5761-c245-452a-9de1-a8e558af10d2,
  abstract     = {{Superantigens (SAgs) are bacterial enterotoxins produced by Staphylococcus aureus. Staphylococcal enterotoxin type A (SEA), a staphylococcal superantigen, has been shown to bind to the cytokine signalling receptor glycoprotein 130 (gp130). The structural details, as well as the exact physiological role of this interaction, remain unclear. Here, we describe the structural details of the SEA–gp130 complex by combining crosslinking mass spectrometry and computational modelling. Interestingly, SEA is not able to bind gp130-homologues from rat and mouse. Our data suggest that SEA may interact with human gp130 in a different manner than other known gp130-ligands. Moreover, the fact that SEA does not bind mouse or rat gp130 suggests that SAgs have additional mechanisms of action in humans.}},
  author       = {{Uzuncayir, Sibel and Vera-Rodriguez, Arturo and Regenthal, Paulina and Åbacka, Hannah and Emanuelsson, Cecilia and Bahl, Christopher D. and Lindkvist-Petersson, Karin}},
  issn         = {{1873-3468}},
  language     = {{eng}},
  number       = {{7}},
  pages        = {{910--923}},
  publisher    = {{Wiley-Blackwell}},
  series       = {{FEBS Letters}},
  title        = {{Analyses of the complex formation of staphylococcal enterotoxin A and the human gp130 cytokine receptor}},
  url          = {{http://dx.doi.org/10.1002/1873-3468.14292}},
  doi          = {{10.1002/1873-3468.14292}},
  volume       = {{596}},
  year         = {{2022}},
}