Composition of carbon, nitrogen and phosphorus in single cells of three filamentous cyanobacteria using nuclear microprobe and traditional techniques
(2013) In Aquatic Microbial Ecology 71(1). p.91-97- Abstract
- Intracellular of carbon, nitrogen and phosphorus content in phytoplankton cells is traditionally measured using concentrates containing thousands to millions of cells. In the present study, we used a nuclear microprobe (NMP) to determine C, N and P concentrations in single filaments of 3 cyanobacteria species: Anabaena sp., Nodularia spumigena and Aphanizomenon flosaquae var. klebahnii isolated from Baltic Sea water. Estimations of C, N and P content per cell were calculated and compared to the concentrations found using traditional bulk methods. No significant differences regarding the C, N and P cellular content were found between the 2 methods for each of the species tested (p < 0.05). From our results, we conclude that the NMP can... (More)
- Intracellular of carbon, nitrogen and phosphorus content in phytoplankton cells is traditionally measured using concentrates containing thousands to millions of cells. In the present study, we used a nuclear microprobe (NMP) to determine C, N and P concentrations in single filaments of 3 cyanobacteria species: Anabaena sp., Nodularia spumigena and Aphanizomenon flosaquae var. klebahnii isolated from Baltic Sea water. Estimations of C, N and P content per cell were calculated and compared to the concentrations found using traditional bulk methods. No significant differences regarding the C, N and P cellular content were found between the 2 methods for each of the species tested (p < 0.05). From our results, we conclude that the NMP can be a useful tool for studying the elemental content of single phytoplankton cells. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4275334
- author
- Blanco, Eva Perez ; Karlsson, Chatarina ; Pallon, Jan LU and Graneli, Edna
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Baltic Sea, Elemental cellular content, Nitrogen-fixing cyanobacteria, Nuclear microprobe, Nutrients
- in
- Aquatic Microbial Ecology
- volume
- 71
- issue
- 1
- pages
- 91 - 97
- publisher
- Inter-Research
- external identifiers
-
- wos:000327552900007
- scopus:84889071168
- ISSN
- 0948-3055
- DOI
- 10.3354/ame01665
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Nuclear Physics (Faculty of Technology) (011013007)
- id
- 622bc627-a381-48db-9646-b7ba3028d463 (old id 4275334)
- date added to LUP
- 2016-04-01 13:28:04
- date last changed
- 2022-01-27 19:19:50
@article{622bc627-a381-48db-9646-b7ba3028d463, abstract = {{Intracellular of carbon, nitrogen and phosphorus content in phytoplankton cells is traditionally measured using concentrates containing thousands to millions of cells. In the present study, we used a nuclear microprobe (NMP) to determine C, N and P concentrations in single filaments of 3 cyanobacteria species: Anabaena sp., Nodularia spumigena and Aphanizomenon flosaquae var. klebahnii isolated from Baltic Sea water. Estimations of C, N and P content per cell were calculated and compared to the concentrations found using traditional bulk methods. No significant differences regarding the C, N and P cellular content were found between the 2 methods for each of the species tested (p < 0.05). From our results, we conclude that the NMP can be a useful tool for studying the elemental content of single phytoplankton cells.}}, author = {{Blanco, Eva Perez and Karlsson, Chatarina and Pallon, Jan and Graneli, Edna}}, issn = {{0948-3055}}, keywords = {{Baltic Sea; Elemental cellular content; Nitrogen-fixing cyanobacteria; Nuclear microprobe; Nutrients}}, language = {{eng}}, number = {{1}}, pages = {{91--97}}, publisher = {{Inter-Research}}, series = {{Aquatic Microbial Ecology}}, title = {{Composition of carbon, nitrogen and phosphorus in single cells of three filamentous cyanobacteria using nuclear microprobe and traditional techniques}}, url = {{http://dx.doi.org/10.3354/ame01665}}, doi = {{10.3354/ame01665}}, volume = {{71}}, year = {{2013}}, }