Modular 3D printed platform for fluidically connected human brain organoid culture
(2024) In Biofabrication 1(15014).- Abstract
Brain organoid technology has transformed both basic and applied biomedical research and paved the way for novel insights into developmental processes and disease states of the human brain. While the use of brain organoids has been rapidly growing in the past decade, the accompanying bioengineering and biofabrication solutions have remained scarce. As a result, most brain organoid protocols still rely on commercially available tools and culturing platforms that had previously been established for different purposes, thus entailing suboptimal culturing conditions and excessive use of plasticware. To address these issues, we developed a 3D printing pipeline for the fabrication of tailor-made culturing platforms for fluidically connected... (More)
Brain organoid technology has transformed both basic and applied biomedical research and paved the way for novel insights into developmental processes and disease states of the human brain. While the use of brain organoids has been rapidly growing in the past decade, the accompanying bioengineering and biofabrication solutions have remained scarce. As a result, most brain organoid protocols still rely on commercially available tools and culturing platforms that had previously been established for different purposes, thus entailing suboptimal culturing conditions and excessive use of plasticware. To address these issues, we developed a 3D printing pipeline for the fabrication of tailor-made culturing platforms for fluidically connected but spatially separated brain organoid array culture. This all-in-one platform allows all culturing steps—from cellular aggregation, spheroid growth, hydrogel embedding, and organoid maturation—to be performed in a single well plate without the need for organoid manipulation or transfer. Importantly, the approach relies on accessible materials and widely available 3D printing equipment. Furthermore, the developed design principles are modular and highly customizable. As such, we believe that the presented technology can be easily adapted by other research groups and fuel further development of culturing tools and platforms for brain organoids and other 3D cellular systems.
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- author
- Rezaei, Babak ; Giacomoni, Jessica LU ; Nilsson, Fredrik LU ; Sozzi, Edoardo LU ; Fiorenzano, Alessandro LU ; Parmar, Malin LU ; Keller, Stephan S. and Kajtez, Janko LU
- organization
- publishing date
- 2024-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 3D culture, 3D printing, brain organoids, modular design, neuroscience, stereolithography
- in
- Biofabrication
- volume
- 1
- issue
- 15014
- article number
- 015014
- publisher
- IOP Publishing
- external identifiers
-
- pmid:37956452
- scopus:85177500554
- ISSN
- 1758-5082
- DOI
- 10.1088/1758-5090/ad0c2c
- language
- English
- LU publication?
- yes
- additional info
- Funding Information: The authors would like to thank Bengt Mattsson for the creation of 3D models and illustrations. Lund University Bioimaging Centre (LBIC) is gratefully acknowledged for providing experimental resources for electron and fluorescence microscopy. B R and S S K acknowledge the financial support from the European Research Council under the Horizon 2020 framework program (Grant No. 772370-PHOENEEX). J K and M P acknowledge the financial support from The Swedish Research Council (2021-02967) and HORIZON-EIC-2021-PATHFINDEROPEN-01 project OpenMIND (101047177). A F is supported by Swedish Research Council (2022-01432) and Italian Ministry of Health (B53C22007860001). Publisher Copyright: © 2023 The Author(s). Published by IOP Publishing Ltd.
- id
- 429f4047-e57a-4f7e-9790-e2bda12d1e12
- date added to LUP
- 2023-12-19 14:41:12
- date last changed
- 2024-09-06 13:20:27
@article{429f4047-e57a-4f7e-9790-e2bda12d1e12, abstract = {{<p>Brain organoid technology has transformed both basic and applied biomedical research and paved the way for novel insights into developmental processes and disease states of the human brain. While the use of brain organoids has been rapidly growing in the past decade, the accompanying bioengineering and biofabrication solutions have remained scarce. As a result, most brain organoid protocols still rely on commercially available tools and culturing platforms that had previously been established for different purposes, thus entailing suboptimal culturing conditions and excessive use of plasticware. To address these issues, we developed a 3D printing pipeline for the fabrication of tailor-made culturing platforms for fluidically connected but spatially separated brain organoid array culture. This all-in-one platform allows all culturing steps—from cellular aggregation, spheroid growth, hydrogel embedding, and organoid maturation—to be performed in a single well plate without the need for organoid manipulation or transfer. Importantly, the approach relies on accessible materials and widely available 3D printing equipment. Furthermore, the developed design principles are modular and highly customizable. As such, we believe that the presented technology can be easily adapted by other research groups and fuel further development of culturing tools and platforms for brain organoids and other 3D cellular systems.</p>}}, author = {{Rezaei, Babak and Giacomoni, Jessica and Nilsson, Fredrik and Sozzi, Edoardo and Fiorenzano, Alessandro and Parmar, Malin and Keller, Stephan S. and Kajtez, Janko}}, issn = {{1758-5082}}, keywords = {{3D culture; 3D printing; brain organoids; modular design; neuroscience; stereolithography}}, language = {{eng}}, month = {{01}}, number = {{15014}}, publisher = {{IOP Publishing}}, series = {{Biofabrication}}, title = {{Modular 3D printed platform for fluidically connected human brain organoid culture}}, url = {{http://dx.doi.org/10.1088/1758-5090/ad0c2c}}, doi = {{10.1088/1758-5090/ad0c2c}}, volume = {{1}}, year = {{2024}}, }