Adipocytes derived from PA6 cells reliably promote the differentiation of dopaminergic neurons from human embryonic stem cells.
(2014) In Journal of Neuroscience Research 92(5). p.564-573- Abstract
- The PA6 stromal cell line comprises a heterogeneous population of cells that can induce both mouse and human embryonic stem cells to differentiate into dopaminergic neurons. This ability of PA6 cells has been termed stromal cell-derived inducing activity (SDIA). The level of SDIA has been found to vary considerably between and within batches of PA6 cells. Not only are the molecular mechanisms that underlie SDIA unknown but also the cell type(s) within the heterogeneous PA6 cultures that underlie SDIA remain poorly defined. In this study, we reveal that adipocytes, which are present within the heterogeneous PA6 cell population, robustly release the factors mediating SDIA. Furthermore, we report that the coculture of human embryonic stem... (More)
- The PA6 stromal cell line comprises a heterogeneous population of cells that can induce both mouse and human embryonic stem cells to differentiate into dopaminergic neurons. This ability of PA6 cells has been termed stromal cell-derived inducing activity (SDIA). The level of SDIA has been found to vary considerably between and within batches of PA6 cells. Not only are the molecular mechanisms that underlie SDIA unknown but also the cell type(s) within the heterogeneous PA6 cultures that underlie SDIA remain poorly defined. In this study, we reveal that adipocytes, which are present within the heterogeneous PA6 cell population, robustly release the factors mediating SDIA. Furthermore, we report that the coculture of human embryonic stem cells with PA6-derived adipocytes reliably induces their differentiation into midbrain dopaminergic neurons. © 2014 Wiley Periodicals, Inc. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4336127
- author
- Guloglu, Oktar LU ; Larsen, Anna and Brundin, Patrik LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Neuroscience Research
- volume
- 92
- issue
- 5
- pages
- 564 - 573
- publisher
- John Wiley & Sons Inc.
- external identifiers
-
- pmid:24482287
- wos:000332553400003
- scopus:84895929762
- pmid:24482287
- ISSN
- 1097-4547
- DOI
- 10.1002/jnr.23355
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuronal Survival (013212041)
- id
- 3c709665-47a7-4eca-a5d4-5007044db433 (old id 4336127)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24482287?dopt=Abstract
- date added to LUP
- 2016-04-01 11:03:26
- date last changed
- 2022-01-26 05:05:05
@article{3c709665-47a7-4eca-a5d4-5007044db433, abstract = {{The PA6 stromal cell line comprises a heterogeneous population of cells that can induce both mouse and human embryonic stem cells to differentiate into dopaminergic neurons. This ability of PA6 cells has been termed stromal cell-derived inducing activity (SDIA). The level of SDIA has been found to vary considerably between and within batches of PA6 cells. Not only are the molecular mechanisms that underlie SDIA unknown but also the cell type(s) within the heterogeneous PA6 cultures that underlie SDIA remain poorly defined. In this study, we reveal that adipocytes, which are present within the heterogeneous PA6 cell population, robustly release the factors mediating SDIA. Furthermore, we report that the coculture of human embryonic stem cells with PA6-derived adipocytes reliably induces their differentiation into midbrain dopaminergic neurons. © 2014 Wiley Periodicals, Inc.}}, author = {{Guloglu, Oktar and Larsen, Anna and Brundin, Patrik}}, issn = {{1097-4547}}, language = {{eng}}, number = {{5}}, pages = {{564--573}}, publisher = {{John Wiley & Sons Inc.}}, series = {{Journal of Neuroscience Research}}, title = {{Adipocytes derived from PA6 cells reliably promote the differentiation of dopaminergic neurons from human embryonic stem cells.}}, url = {{http://dx.doi.org/10.1002/jnr.23355}}, doi = {{10.1002/jnr.23355}}, volume = {{92}}, year = {{2014}}, }