Bacillus subtilis CtaA is a heme-containing membrane proteininvolved in heme A biosynthesis
(1994) In Journal of Bacteriology 176(21). p.6663-6671- Abstract
- Heme A is a prosthetic group of many respiratory oxidases. It is synthesized from protoheme IX (heme B) seemingly with heme O as a stable intermediate. The Bacillus subtilis ctaA and ctaB genes are required for heme A and heme O synthesis, respectively (B. Svensson, M. Lubben, and L. Hederstedt, Mol. Microbiol. 10:193-201, 1993). Tentatively, CtaA is involved in the monooxygenation and oxidation of the methyl side group on porphyrin ring D in heme A synthesis from heme B. B. subtilis ctaA and ctaB on plasmids in both B. subtilis and Escherichia coli were found to result in a novel membrane-bound heme-containing protein with the characteristics of a low-spin b type cytochrome. It fan be reduced via the respiratory chain, and in the reduced... (More)
- Heme A is a prosthetic group of many respiratory oxidases. It is synthesized from protoheme IX (heme B) seemingly with heme O as a stable intermediate. The Bacillus subtilis ctaA and ctaB genes are required for heme A and heme O synthesis, respectively (B. Svensson, M. Lubben, and L. Hederstedt, Mol. Microbiol. 10:193-201, 1993). Tentatively, CtaA is involved in the monooxygenation and oxidation of the methyl side group on porphyrin ring D in heme A synthesis from heme B. B. subtilis ctaA and ctaB on plasmids in both B. subtilis and Escherichia coli were found to result in a novel membrane-bound heme-containing protein with the characteristics of a low-spin b type cytochrome. It fan be reduced via the respiratory chain, and in the reduced state it shows light absorption maxima at 428, 528, and 558 nm and the or-band is split. Purified cytochrome isolated from both B. subtilis and E. coli membranes contained one polypeptide identified as CtaA by amino acid sequence analysis, about 0.2 mol of heme B per mol of polypeptide, and small amounts of heme A. (Less)
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- author
- Svensson, B. and Hederstedt, Lars LU
- organization
- publishing date
- 1994
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Journal of Bacteriology
- volume
- 176
- issue
- 21
- pages
- 6663 - 6671
- publisher
- American Society for Microbiology
- external identifiers
-
- scopus:0028046467
- ISSN
- 0021-9193
- DOI
- 10.1128/jb.176.21.6663-6671.1994
- language
- English
- LU publication?
- yes
- id
- 44846ede-d441-4e41-b7e9-5579df433b3c
- date added to LUP
- 2017-07-18 10:15:02
- date last changed
- 2021-09-05 04:58:04
@article{44846ede-d441-4e41-b7e9-5579df433b3c, abstract = {{Heme A is a prosthetic group of many respiratory oxidases. It is synthesized from protoheme IX (heme B) seemingly with heme O as a stable intermediate. The Bacillus subtilis ctaA and ctaB genes are required for heme A and heme O synthesis, respectively (B. Svensson, M. Lubben, and L. Hederstedt, Mol. Microbiol. 10:193-201, 1993). Tentatively, CtaA is involved in the monooxygenation and oxidation of the methyl side group on porphyrin ring D in heme A synthesis from heme B. B. subtilis ctaA and ctaB on plasmids in both B. subtilis and Escherichia coli were found to result in a novel membrane-bound heme-containing protein with the characteristics of a low-spin b type cytochrome. It fan be reduced via the respiratory chain, and in the reduced state it shows light absorption maxima at 428, 528, and 558 nm and the or-band is split. Purified cytochrome isolated from both B. subtilis and E. coli membranes contained one polypeptide identified as CtaA by amino acid sequence analysis, about 0.2 mol of heme B per mol of polypeptide, and small amounts of heme A.}}, author = {{Svensson, B. and Hederstedt, Lars}}, issn = {{0021-9193}}, language = {{eng}}, number = {{21}}, pages = {{6663--6671}}, publisher = {{American Society for Microbiology}}, series = {{Journal of Bacteriology}}, title = {{<em>Bacillus subtilis</em> CtaA is a heme-containing membrane proteininvolved in heme A biosynthesis}}, url = {{http://dx.doi.org/10.1128/jb.176.21.6663-6671.1994}}, doi = {{10.1128/jb.176.21.6663-6671.1994}}, volume = {{176}}, year = {{1994}}, }