Countercurrent Distribution of Lymphocytes from Human Peripheral Blood in an Aqueous Two-Phase System : I. Separation into Subsets of Lymphocytes Bearing Distinctive Markers’
(1980) In Cellular Immunology 53. p.39-50- Abstract
- Lymphocytes from human peripheral blood have been separated by countercurrent distribution in a charged aqueous two-phase system composed of Dextran T 500 and polyethylene glycol 6000 with a cell yield of 59–88% and viability above 90%. A highly reproducible partition pattern was seen with four distinct peaks. Lymphocytes with surface membrane immunoglobulin (SmIg) were located in the first part of the distribution corresponding mainly to peak I. T lymphocytes as detected by E rosetting and α-naphthyl acetate esterase (ANAE) staining showed a broad distribution with a maximum in peaks II and III. ANAE-negative lymphocytes were seen in both extremes of the distribution, corresponding to B cells in the first part and to a population of E−... (More)
- Lymphocytes from human peripheral blood have been separated by countercurrent distribution in a charged aqueous two-phase system composed of Dextran T 500 and polyethylene glycol 6000 with a cell yield of 59–88% and viability above 90%. A highly reproducible partition pattern was seen with four distinct peaks. Lymphocytes with surface membrane immunoglobulin (SmIg) were located in the first part of the distribution corresponding mainly to peak I. T lymphocytes as detected by E rosetting and α-naphthyl acetate esterase (ANAE) staining showed a broad distribution with a maximum in peaks II and III. ANAE-negative lymphocytes were seen in both extremes of the distribution, corresponding to B cells in the first part and to a population of E− and SmIg− lymphocytes in the last part. Monocytes were present in all fractions with some enrichment in peaks II–IV. Lymphocytes with low-affinity Fc receptors were found in B-cell-containing fractions in the first part of the distribution, but also in the last part. Lymphocytes with high-affinity Fc receptors were detected mainly in peak IV. It is thus demonstrated that peripheral blood lymphocytes can be fractionated into subpopulations enriched in cells with characteristic markers.
markers.
(Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/44a1b9d5-efe3-4976-8988-3b733a63111b
- author
- Malmström, P. LU ; Jönsson, Å. ; Hallberg, T. and Sjögren, H.O.
- organization
- publishing date
- 1980
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Cellular Immunology
- volume
- 53
- pages
- 39 - 50
- publisher
- Elsevier
- external identifiers
-
- scopus:0018867681
- ISSN
- 0008-8749
- DOI
- 10.1016/0008-8749(80)90424-4
- language
- English
- LU publication?
- yes
- id
- 44a1b9d5-efe3-4976-8988-3b733a63111b
- date added to LUP
- 2019-08-29 13:48:03
- date last changed
- 2023-07-10 04:05:01
@article{44a1b9d5-efe3-4976-8988-3b733a63111b,
abstract = {{Lymphocytes from human peripheral blood have been separated by countercurrent distribution in a charged aqueous two-phase system composed of Dextran T 500 and polyethylene glycol 6000 with a cell yield of 59–88% and viability above 90%. A highly reproducible partition pattern was seen with four distinct peaks. Lymphocytes with surface membrane immunoglobulin (SmIg) were located in the first part of the distribution corresponding mainly to peak I. T lymphocytes as detected by E rosetting and α-naphthyl acetate esterase (ANAE) staining showed a broad distribution with a maximum in peaks II and III. ANAE-negative lymphocytes were seen in both extremes of the distribution, corresponding to B cells in the first part and to a population of E− and SmIg− lymphocytes in the last part. Monocytes were present in all fractions with some enrichment in peaks II–IV. Lymphocytes with low-affinity Fc receptors were found in B-cell-containing fractions in the first part of the distribution, but also in the last part. Lymphocytes with high-affinity Fc receptors were detected mainly in peak IV. It is thus demonstrated that peripheral blood lymphocytes can be fractionated into subpopulations enriched in cells with characteristic markers.<br/>markers. <br/>}},
author = {{Malmström, P. and Jönsson, Å. and Hallberg, T. and Sjögren, H.O.}},
issn = {{0008-8749}},
language = {{eng}},
pages = {{39--50}},
publisher = {{Elsevier}},
series = {{Cellular Immunology}},
title = {{Countercurrent Distribution of Lymphocytes from Human Peripheral Blood in an Aqueous Two-Phase System : I. Separation into Subsets of Lymphocytes Bearing Distinctive Markers’}},
url = {{http://dx.doi.org/10.1016/0008-8749(80)90424-4}},
doi = {{10.1016/0008-8749(80)90424-4}},
volume = {{53}},
year = {{1980}},
}