Ovarian carcinoma histotype determination is highly reproducible, and is improved through the use of immunohistochemistry
(2014) In Histopathology 64(7). p.1004-1013- Abstract
- AimsTo assess the variation in ovarian carcinoma type diagnosis among gynaecological pathologists from Nordic countries, and whether a rationally designed panel of immunohistochemical markers could improve diagnostic reproducibility. Methods and resultsEight pathologists from four countries (Sweden, Denmark, Norway, and Finland) received an educational lecture on the diagnosis of ovarian carcinoma type. All tumour-containing slides from 54 ovarian carcinoma cases were independently reviewed by the participants, who: (i) determined type purely on the basis of histology; (ii) indicated whether they would apply immunohistochemistry in their routine practice; and (iii) determined type after reviewing the staining results. The results for six... (More)
- AimsTo assess the variation in ovarian carcinoma type diagnosis among gynaecological pathologists from Nordic countries, and whether a rationally designed panel of immunohistochemical markers could improve diagnostic reproducibility. Methods and resultsEight pathologists from four countries (Sweden, Denmark, Norway, and Finland) received an educational lecture on the diagnosis of ovarian carcinoma type. All tumour-containing slides from 54 ovarian carcinoma cases were independently reviewed by the participants, who: (i) determined type purely on the basis of histology; (ii) indicated whether they would apply immunohistochemistry in their routine practice; and (iii) determined type after reviewing the staining results. The results for six markers (WT1, p53, p16, HNF-1, ARID1A, and progesterone receptor) were determined for all 54 cases, by staining of a tissue microarray. The median concordance with central review diagnosis was 86%, and significantly improved to 90% with the incorporation of immunostaining results (P=0.0002). The median interobserver agreement was 78%, and significantly improved to 85% with the incorporation of immunostaining results (P=0.0002). ConclusionsUse of the immunostaining results significantly improved both diagnostic accuracy and interobserver agreement. These results indicate that ovarian carcinoma type can be reliably diagnosed by pathologists from different countries, and also demonstrate that immunohistochemistry has an important role in improving diagnostic accuracy and agreement between pathologists. (Less)
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https://lup.lub.lu.se/record/4558937
- author
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- clear cell adenocarcinoma, cystic neoplasm, endometrioid carcinoma, mucinous neoplasm, ovary, reproducibility of results, serous neoplasm
- in
- Histopathology
- volume
- 64
- issue
- 7
- pages
- 1004 - 1013
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000335729700009
- scopus:84899943779
- ISSN
- 0309-0167
- DOI
- 10.1111/his.12349
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Pathology, (Lund) (013030000)
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- 221095f1-fabf-4872-9f2b-d78fb7b71969 (old id 4558937)
- date added to LUP
- 2016-04-01 10:47:09
- date last changed
- 2022-09-15 12:35:42
@article{221095f1-fabf-4872-9f2b-d78fb7b71969,
abstract = {{AimsTo assess the variation in ovarian carcinoma type diagnosis among gynaecological pathologists from Nordic countries, and whether a rationally designed panel of immunohistochemical markers could improve diagnostic reproducibility. Methods and resultsEight pathologists from four countries (Sweden, Denmark, Norway, and Finland) received an educational lecture on the diagnosis of ovarian carcinoma type. All tumour-containing slides from 54 ovarian carcinoma cases were independently reviewed by the participants, who: (i) determined type purely on the basis of histology; (ii) indicated whether they would apply immunohistochemistry in their routine practice; and (iii) determined type after reviewing the staining results. The results for six markers (WT1, p53, p16, HNF-1, ARID1A, and progesterone receptor) were determined for all 54 cases, by staining of a tissue microarray. The median concordance with central review diagnosis was 86%, and significantly improved to 90% with the incorporation of immunostaining results (P=0.0002). The median interobserver agreement was 78%, and significantly improved to 85% with the incorporation of immunostaining results (P=0.0002). ConclusionsUse of the immunostaining results significantly improved both diagnostic accuracy and interobserver agreement. These results indicate that ovarian carcinoma type can be reliably diagnosed by pathologists from different countries, and also demonstrate that immunohistochemistry has an important role in improving diagnostic accuracy and agreement between pathologists.}},
author = {{Koebel, Martin and Bak, Julia and Bertelsen, Bjorn I. and Carpen, Olli and Grove, Anni and Hansen, Estrid S. and Jakobsen, Anne-Marie Levin and Lidang, Marianne and Måsbäck, Anna and Tolf, Anna and Gilks, C. Blake and Carlson, Joseph W.}},
issn = {{0309-0167}},
keywords = {{clear cell adenocarcinoma; cystic neoplasm; endometrioid carcinoma; mucinous neoplasm; ovary; reproducibility of results; serous neoplasm}},
language = {{eng}},
number = {{7}},
pages = {{1004--1013}},
publisher = {{Wiley-Blackwell}},
series = {{Histopathology}},
title = {{Ovarian carcinoma histotype determination is highly reproducible, and is improved through the use of immunohistochemistry}},
url = {{http://dx.doi.org/10.1111/his.12349}},
doi = {{10.1111/his.12349}},
volume = {{64}},
year = {{2014}},
}