Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature
(2014) In Langmuir 30(25). p.7259-7263- Abstract
- We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature on the subject, high-quality bilayers can be formed below the main phase-transition temperature of the lipid. We have carefully studied the formation mechanism of supported DPPC bilayers below and above the lipid melting temperature (T-m) by quartz crystal microbalance and atomic force microscopy under continuous flow conditions. We also measured the structure of lipid bilayers formed below or above T-m by neutron reflection and investigated the effect of subsequent cooling to below the T-m. Our results clearly... (More)
- We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature on the subject, high-quality bilayers can be formed below the main phase-transition temperature of the lipid. We have carefully studied the formation mechanism of supported DPPC bilayers below and above the lipid melting temperature (T-m) by quartz crystal microbalance and atomic force microscopy under continuous flow conditions. We also measured the structure of lipid bilayers formed below or above T-m by neutron reflection and investigated the effect of subsequent cooling to below the T-m. Our results clearly show that a continuous supported bilayer can be formed with high surface coverage below the lipid T-m. We also demonstrate that the high dissipation responses observed during the deposition process by QCM-D correspond to vesicles absorbed on top of a continuous bilayer and not to a surface-supported vesicular layer as previously reported. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4602814
- author
- Lind, Tania Kjellerup ; Cardenas, Marite and Wacklin, Hanna LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Langmuir
- volume
- 30
- issue
- 25
- pages
- 7259 - 7263
- publisher
- The American Chemical Society (ACS)
- external identifiers
-
- wos:000338488600001
- scopus:84903748464
- pmid:24932971
- ISSN
- 0743-7463
- DOI
- 10.1021/la500897x
- language
- English
- LU publication?
- yes
- id
- 46002feb-e68a-4297-acc1-c62ba0a8ec8d (old id 4602814)
- date added to LUP
- 2016-04-01 10:18:28
- date last changed
- 2022-02-17 08:49:43
@article{46002feb-e68a-4297-acc1-c62ba0a8ec8d,
abstract = {{We have investigated the effect of deposition temperature on supported lipid bilayer formation via vesicle fusion. By using several complementary surface-sensitive techniques, we demonstrate that despite contradicting literature on the subject, high-quality bilayers can be formed below the main phase-transition temperature of the lipid. We have carefully studied the formation mechanism of supported DPPC bilayers below and above the lipid melting temperature (T-m) by quartz crystal microbalance and atomic force microscopy under continuous flow conditions. We also measured the structure of lipid bilayers formed below or above T-m by neutron reflection and investigated the effect of subsequent cooling to below the T-m. Our results clearly show that a continuous supported bilayer can be formed with high surface coverage below the lipid T-m. We also demonstrate that the high dissipation responses observed during the deposition process by QCM-D correspond to vesicles absorbed on top of a continuous bilayer and not to a surface-supported vesicular layer as previously reported.}},
author = {{Lind, Tania Kjellerup and Cardenas, Marite and Wacklin, Hanna}},
issn = {{0743-7463}},
language = {{eng}},
number = {{25}},
pages = {{7259--7263}},
publisher = {{The American Chemical Society (ACS)}},
series = {{Langmuir}},
title = {{Formation of Supported Lipid Bilayers by Vesicle Fusion: Effect of Deposition Temperature}},
url = {{http://dx.doi.org/10.1021/la500897x}},
doi = {{10.1021/la500897x}},
volume = {{30}},
year = {{2014}},
}